بررسی تعاملات بین گونه‌ای بخش زیرزمینی در کشت مخلوط گیاه سویا (Glycine max (L.) Merrill) و دان‌سیاه (Guizotia abyssinica Cass.)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه زراعت، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

2 گروه زراعت دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

3 پژوهشکده ژنتیک و زیست‌فناوری طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران.منابع طبیعی ساری

4 گروه زراعت، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران.

چکیده

ارتباط بین گونه­ای گیاهان در بخش زیرزمینی یک موضوع کلیدی جهت شناسایی ساز و کار رقابت و بهره­وری در کشت مخلوط است. بنابراین، به‌منظور ارزیابی تعاملات بخش زیرزمینی در نسبت­های مختلف کشت مخلوط گیاه دان­سیاه (Guizotia abyssinica Cass) و سویا (Glycine max (L.) Merrill) آزمایشی در دو سال زراعی (97- 1396و 98- 1397) و به‌صورت طرح بلوک­های کامل تصادفی با چهار تکرار در مزرعه تحقیقاتی دانشگاه علوم کشاورزی و منابع طبیعی ساری اجرا شد. تیمارهای آزمایش شامل نسبت­های کاشت 0:100، 25:75، 50:50، 75:25 و 100:0 (به‌ترتیب دان­سیاه- سویا) به‌روش جایگزینی بودند. بر اساس نتایج به‌دست آمده، نسبت­های مختلف کاشت اثر معنی‌داری بر درصد نیتروژن حاصل از تثبیت بیولوژیکی، میزان فعالیت کل میکروبی خاک، فعالیت ریشه و مقدار فنول و فلاونوئید موجود در عصاره ریشه گیاه دان­سیاه داشتند. تمامی نسبت­های مختلف کشت مخلوط در مقایسه با تیمار کشت خالص دارای قابلیت تثبیت بیولوژیکی نیتروژن بیشتری بودند. بیشترین افزایش درصد نیتروژن حاصل از تثبیت بیولوژیکی در هر دو سال زراعی مربوط به مرحله 90 روز پس از کاشت بود. در این مرحله نسبت کاشت 50:50 به‌ترتیب در سال­های مورد مطالعه با میانگین 46/85 و 58/91 درصد بیشترین درصد نیتروژن حاصل از تثبیت بیولوژیکی را در اختیار داشت. علاوه‌بر مطالب مذکور مجموع فعالیت کل میکروبی خاک در نسبت­های مختلف کشت مخلوط بیشتر از کشت خالص گیاهان مورد مطالعه بود و در مرحله 60 روز پس از کاشت نسبت کاشت 50:50 با میانگین 56/34 (g.Fluorecsceine.g-1.h-1μ) دارای بیشترین میزان فعالیت میکروبی خاک بود. همچنین گیاه دان­سیاه در نسبت­های مختلف کشت مخلوط ضمن افزایش فعالیت ریشه از میزان فنل و فلاونوئید بیشتری نیز در عصاره ریشه برخوردار بود. نسبت­های مختلف کشت مخلوط نیز از نظر تولید ترکیبات ثانویه موفق­تر از کشت خالص گیاه دان­سیاه عمل کردند. در نهایت، افزایش معنی­داری درصد نیتروژن حاصل از تثبیت بیولوژیکی و علاوه‌بر آن فعالیت بیشتر ریشه گیاه دان­سیاه به‌عنوان گیاه مکمل در نسبت­های کشت مخلوط می­تواند نقش مهمی در پویایی تعاملات بین­گونه­ای در بخش زیرزمینی در الگوی کشت مخلوط داشته باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Investigation of Below-Ground Interspecific Interaction in the Intercropping Culture of Soybean (Glycine max (L.) Merrill) and Niger (Guizotia abyssinica Cass.)

نویسندگان [English]

  • Meisam Namdari 1
  • Rahmat Abbasi 2
  • Hemmatollah Pirdashti 3
  • Faezeh Zaefarian 4
1 Department of Agronomy, Sari University of Agricultural Sciences and Natural Resources, Sari, Iran.
2 Department of Agronomy, Sari University of Agricultural Sciences and Natural Resources, Sari, Iran.
3 Department of Genetic & Agricultural Biotechnologhy Institute of Tabarestan, Sari University of Agricultural Sciences and Natural Resources, Sari, Iran
4 Department of Agronomy, Sari University of Agricultural Sciences and Natural Resources, Sari, Iran.
چکیده [English]

Introduction
Most research on intercropping cultivation in the past has focused on seed yield and yield components, and less attention has been paid to below-ground interaction processes. However, the connection of plants in the below-ground sector is a vital issue in identifying the mechanism of competition and productivity in intercropping cultivation. Meanwhile, the selection of a legume plant due to the biological nitrogen fixation (BNF) increases the productivity in intercropping cultivation. However, the BNF by the legume component can be affected by the component crop in intercropping cultivation. Therefore, creating the optimum conditions for the BNF in planting ratios plays an essential role in the dynamics of inter-species interaction. Therefore, this study aimed to evaluate the interactions of the below-ground sector and the percentage of nitrogen derived from the atmosphere in different ratios of intercropping cultivation.
Material and Methods
A field experiment was conducted for two years (2018-19) at the research farm of Sari agricultural science and natural resources university, Iran (33º, 36ʹ N, 53º, 03ʹ E with 43 m altitude). The experimental design was a randomized complete block with four replications. The experimental treatment was 75% soybean + 25% niger, 50% soybean + 50% niger, 25% Soybean+75% niger based on the replacement method and monoculture. For example, a 25:75 combination consisted of three rows of planting a soybean and one row of niger and vice versa in the 75:25 combinations. The 50:50 also included planting two rows of each of the studied plants. This study investigated the percentage of nitrogen derived from the atmosphere, nitrogen yield, total enzymatic activity of the soil, and root activity in different planting ratios.
Result and Discussion
The percentage of nitrogen derived from the atmosphere (Ndfa) in all different planting ratios was higher than a single soybean crop. This trend continued until it reached 90 days after planting. At 75 and 90 days after planting, the highest Ndfa has related to 50:50 and 75:25 planting ratios in both cultivation years. Also, the yield of Ndfa was higher than expected in planting ratios, and its value increased further as it approached the final stages of growth. In addition to the above, the total microbial activity of soil in different ratios of intercropping cultivation was higher than the monoculture of the studied plants. Its amount in 50:50 planting ratio was 16.38 and 31.69% higher than monoculture soybean (0: 100) and niger (0: 100) at 60 days after planting, respectively. The most increased total microbial activity of the ground in niger and soybean plants in the middle and late stages of growth was observed at a depth of 0-20 and 10-30 cm, respectively. Also, niger plant in different ratios of intercropping cultivation, while increasing root activity, have a higher number of secondary compounds in root extract. In general, the increase in root activity in the late stages of growth was accompanied by a decrease in the number of rows of niger plants, especially in the planting ratio of 75:25. Also, the total phenol and flavonoids in the niger root extract increased to 105 days after planting and then decreased. Thus, in general, different ratios of intercropping cultivation were more successful in producing secondary compounds than the monoculture of niger.
Conclusion
Increasing the significance of nitrogen derived from the atmosphere and the other activity of niger root as a complementary plant in intercropping cultivation ratios can play an essential role in the dynamics of underground sector interactions intercropping cultivation patterns. In addition to the above results and based on the total microbial activity of the soil, the complementary structure of niger and soybean root in intercropping cultivation can affect the ability of interspecific competition in intercropping cultivation by distinguishing ecological niche even in a short period.
 

کلیدواژه‌ها [English]

  • nitrogen derived from the atmosphere
  • root extract
  • secondary compounds
  • total microbial activity

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  • تاریخ دریافت: 16 خرداد 1400
  • تاریخ بازنگری: 20 شهریور 1400
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