برهم‌کنش انواع کود و تنش خشکی بر خصوصیات مورفوفیزیولوژیکی و عملکرد گل مغربی (Oenothera biennis L.)

نوع مقاله : علمی - پژوهشی


1 گروه زراعت، دانشگاه تربیت مدرس، تهران، ایران

2 گروه زراعت، پژوهشکده ژنتیک و زیست فناوری طبرستان، دانشگاه علوم کشاورزی ومنابع طبیعی ساری، ایران

3 بخش تحقیقات اصلاح و تهیه نهال و بذر، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی تهران، سازمان تحقیقات، آموزش و ترویج کشاورزی، ورامین، ایران


تغییرات شرایط محیطی طی دوره­ی رشد گیاه غیرقابل پیش­بینی است و از این‌رو ایجاد تنش در هنگام رشد گیاه امری اجتناب­ناپذیر می­باشد؛ یکی از مهم‌ترین این تنش­ها، تنش کم­آبی می­باشد. بنابراین، برای بهبود بهره­وری رشد و تولید گیاه در شرایط تنش، علاوه بر اعمال نهاده­های کشاورزی مناسب (کاربرد کودهای شیمیایی و بیولوژیکی)، نیاز به شناخت و درک فرآیندهای مورفوفیزیولوژیکی گیاه می‌باشد. در همین زمینه آزمایشی با هدف بررسی اثر برهم‌کنش انواع کود و تنش خشکی بر خصوصیات مورفوفیزیولوژیکی و عملکرد گل مغربی (Oenothera biennis L.) به‌صورت مزرعه­ای در دو شهرستان تهران و ورامین در سال 94-1393 انجام شد. آزمایش به‌صورت اسپلیت فاکتوریل در قالب طرح بلوک­های کامل تصادفی در سه تکرار اجرا شد. عامل اصلی شامل سه تیمار بدون تنش آبی (50% از ظرفیت زراعی)، تنش متوسط (40% از ظرفیت زراعی) و تنش شدید (30% از ظرفیت زراعی) بود و عوامل فرعی شامل کاربرد کود شیمیایی در سه سطح (بدون مصرف کود، مصرف 50 و 100% نیتروژن + فسفر مورد نیاز)، قارچ میکوریزا و باکتری آزوسپیریلوم (کاربرد و عدم کاربرد) در نظر گرفته شد. اعمال تنش کم­آبی (متوسط و شدید) باعث کاهش ارتفاع بوته، وزن خشک ساقه، شاخص سطح برگ، عملکرد دانه و محتوای نسبی آب شد. بالاترین میزان کارایی مصرف آب در تنش کم­آبی متوسط و بیش‌ترین میزان وزن خشک ریشه، نسبت وزن خشک ریشه به اندام هوایی و آنزیم کاتالاز در تنش کم­آبی شدید مشاهده شد. هم‌چنین نتایج نشان داد کاربرد کودهای شیمیایی (نیتروژن و فسفر) و بیولوژیکی (میکوریزا و آزوسپیریلوم) می­تواند اثرات منفی تنش کم­آبی را بر عملکرد گل مغربی کاهش داده و سبب بهبود رشد آن شود.


عنوان مقاله [English]

Interaction Effects of Fertilizers and Drought Stress on Morpho-Physiological Characteristics and Yield of Oenothera biennis L

نویسندگان [English]

  • Mahmood Mohammadi 1
  • Seyed Ali Mohammad Modarres-Sanavy 1
  • Hemmatollah Pirdashti 2
  • Zeinolabedin Tahmasebi Sarvestani 1
  • Behnam Zand 3
1 Department of Agronomy, Tarbiat Modares University, Tehran, Iran
2 Department of Agronomy and Plant Breeding, Genetic and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, Sari
3 eed and Plant Improvement Research Department, Tehran Agricultural and Natural Resources Research and Education Center, AREEO, Varamin, Iran
چکیده [English]

To improve crops productivity under drought stress conditions, not only proper management of inputs in time, amount and form, but also the understanding of important physiological processes and defense mechanisms are required to avoid drought stress. Most plant species have the ability to form a symbiosis relationship with the Arbuscular mycorrhizal fungi. AMF could increase growth and yield of plants under water stress, and it is one of the most important biological fertilizers which can be used in mycorrhizal and Azospirillum fields. Research results have shown that mycorrhizal and Azospirillum fungi can moderate the adverse effects of drought stress in plants. One of the most important effects in terms of drought stress is changing the levels of some hormones in plant such as acetic acid and indole acetic acid. Other effects are direct absorption of water by fungal hyphae in soil and its transfer to the host plant, increasing leaf gas exchange, photosynthesis rate as well as enhance the activity of antioxidant enzymes in corn, excellent nitrate and phosphorus assimilation. In addition, extend the water absorption by increasing the leaf water hydration, leaf photosynthesis activity, osmotic regulation and changes in cell membrane flexibility. Hence, the current study was aimed to evaluate chemical fertilizers (nitrogen + phosphorus) and biological fertilizers (Azospirillum and mycorrhizal fungi) effects on water deficit stress on morpho-physiological traits and yield of evening primrose at different experimental stations (Tehran and Varamin regions).
Material and Methods
Two experiments were conducted at two experimental stations in Tehran and Varamin in 2014 and 2015. Experiments were conducted in a split factorial layout within a randomized complete block design with three replications. Three irrigation regime treatments (IR 50 % FC, IR 40 % FC and IR 30 % FC; 50, 40 and 30 percent of field capacity) were randomized to the main plots. Subplots were consisted of factorial combinations of three chemical fertilizers (CF 0, CF 50 % and CF 100 %: no-application, 50 and 100 percent of nitrogen + phosphorus needed by plant) as well as four biological fertilizers (“-M and –Az” and “+M and +Az”: non-inoculated and inoculated with mycorrhizal and Azospirillum).
Results and Discussion
Water stress (moderate and severe) reduced plant height, shoot dry weight, leaf area index, seed yield, phosphorus content and relative water content. However, the highest water use efficiency was obtained at moderate water deficit stress. In addition, the highest root dry weight, root ratio shoot dry weight and catalase were obtained at severe water deficit stress. The application of chemical fertilizers and biological fertilizers in all cases mitigated the negative effects of water deficit stress on plant height, shoot dry weight, root dry weight, root ratio shoot dry weight, leaf area index, seed yield, phosphorus content, catalase, relative water content and water use efficiency traits. Water use efficiency of evening primrose increased with moderate water deficit, application of chemical (Nitrogen + Phosphorus) and biological (mycorrhizal fungi and Azospirillum) fertilizers.
 The compelling results of this study revealed that the inoculation mycorrhizal and Azospirillum could improve evening primrose growth under water deficit stress. Results showed that the application of biological fertilizers can improve evening primrose growth under drought stress. Moreover, mycorrhiza symbioses can increase absorption of moisture and nutrients, reduced irrigation requirements, enhancement drought resistance, more access to food in new places, production of thinner roots, increase root length and nitrogen fixation, which lead to reduction of drought detrimental effects.

کلیدواژه‌ها [English]

  • Azospirillum
  • Mycorrhizal
  • Biological fertilizer
  • Water Deficit Stress and Yield
Abdalla, M.M., and Khoshiban, N.H., 2007. The influence of water stress on growth, relative water content, photosynthetic pigments, some metabolic and hormonal contents of two Triticium aestivum cultivars. Journal of Applied Sciences Research 3: 2062-2074.
Ahmed, C.B., Rouina, B.B., Sensoy, S., Boukhris, M., and Abdallah, F.B., 2009. Changes in gas exchange, proline accumulation and antioxidative enzyme activities in three olive cultivars under contrasting water availability regimes. Environmental and Experimental Botany 67: 345-352.
Asrar, A., and Elhindi, K.M., 2011. Alleviation of drought stress of marigold (Tagetes erecta) plants by using Arbuscular mycorrhizal fungi. Saudi Journal Biologic Science 18: 93-98.
Baghbani-Arania, A., Modarres-Sanavya, S.A.M., Akbar-Boojarb, M.M., and Mokhtassi-Bidgolia, A., 2017. Towards improving the agronomic performance, chlorophyll fluorescence parameters and pigments in fenugreek using zeolite and vermicompost under deficit water stress. Industrial Crops and Production 109: 346-357.
Caravaca, F., Alguacil, M.M., Herna´ndez, J.A., and Rolda´n, A., 2005. Involvement of antioxidant enzyme and nitrate reductase activities during water stress and recovery of mycorrhizal Myrtus communis and Phillyrea angustifolia. Plant Science 169: 191-197.
Chu, G., Chen, T., Wang, Z., Yang, J., and Zhang, J., 2014. Morphological and physiological traits of roots and their relationships with water productivity in water-saving and drought-resistant rice. Field Crops Research 165: 36-48.
Elhindi, K.M., El-Din, A.S., Elgorban, A.M., 2017. The impact of Arbuscular mycorrhizal fungi in mitigating salt-induced adverse effects in sweet basil (Ocimum basilicum L.). Saudi Journal Biologic Science 24: 170-179.
Fieldsend, A., and Morison, J.I.L., 2001. Contrasting growth and dry matter partitioning in winter and spring evening primrose (Oenothera spp.). Field Crops Research 68: 9-20.
Ghasemnezhad, A., and Honermeier, B., 2008. Yield, oil constituents, and protein content of evening primrose (Oenothera biennis L.) seeds depending on harvest time, harvest method and nitrogen application. Industrial Crops Production 28: 17-23.
Goodarzi, F., and Tohidinejad, E.A., 2018. Evaluation of yield potential of common millet (Panicum miliaceum L.) ecotypes under normal and water deficit conditions. Journal of Agroecology 10(1): 135-145. (In Persian with English Summary)
Hazrati, S., Tahmasebi-Sarvestani, Z., Modarres-Sanavy, S.A.M., Mokhtassi-Bidgoli, A., and Nicola, S., 2017. Effects of water stress and light intensity on chlorophyll fluorescence parameters and pigments of Aloe vera L. Plant Physiology and Biochemistry 106: 141-148.
Liu, C., Yang, Z., and Hu, Y.G., 2015. Drought resistance of wheat alien chromosome addition lines evaluated by membership function value based on multiple traits and drought resistance index of grain yield. Field Crops Research 179: 103-112.
Merlos, M.A., Zitka, O., Vojtech, A., Azcon-Aguilar, C., and Ferrol, N., 2016. The arbuscular mycorrhizal fungus Rhizophagus irregularis differentially regulates the copper response of two maize cultivars differing in copper tolerance. Plant Science 253: 68-76.
Mokhtassi-Bidgoli, A., AghaAlikhani, M., Nassiri-Mahallati, M., Zand, E., Gonzalez-Andujar, J.L., and Azari, A., 2013. Agronomic performance, seed quality and nitrogen uptake of Descurainia sophia in response to different nitrogen rates and water regimes. Industrial Crops and Production 44: 583-592.
Mouradi, M., Bouizgaren, A., Farissi, M., Latrach, L., Qaddoury, A., and Ghoulam, C., 2016. Seed osmopriming improves plant growth, nodulation, chlorophyll fluorescence and nutrient uptake in alfalfa (Medicago sativa L.) rhizobia symbiosis under drought stress. Science Horticulture 213: 232-242.
Piccinin, G.G., Braccini, A.L., Dan, L.G., Scapim, C.A., Ricci, T.T., and Bazo, G.L., 2013. Efficiency of seed inoculation with Azospirillum brasilense on agronomic characteristics and yield of wheat. Industrial Crops Production 43: 393-397.
Pirzad, A., Shakiba, M.R., Zehtab-Salmasi, S., Mohammadi, S.A., Darvishzadeh, R., and Samadi, A., 2011. Effect of water stress on leaf relative water content, chlorophyll, proline and soluble carbohydrates in Matricaria chamomilla L. Journal of Medicinal Plants Research 5: 2483-2488.
Shahryari, R., 2018. Quantitative and qualitative comparison of wheat genotypes (Triticum aestivum L.) under terminal water stress conditions and foliar application of humic acid. Journal of Agroecology 10(1): 175-185 (In Persian with English Summary)
Sheng, Q., Zou, Y.N., and Xia, R.X., 2006. Effects of water stress and Arbuscular mycorrhizal fungi on reactive oxygen metabolism and antioxidant production by citrus (Citrus tangerine) roots. Plant Science 169: 191-197.
Tahramooz, H., and Ghalavand, A., 2018. Reducing the effects of water stress using vermicompost and mineral zeolite in Sunflower (Helianthus annus L.). Journal of Agroecology 10(1): 81-93. (In Persian with English Summary)
Vurukonda, S.S.K.P., Vardharajula, S., Shrivastava, M., and SkZ, A., 2016. Enhancement of drought stress tolerance in crops by plant growth promoting rhizobacteria. Microbiological Research 184: 13-24.
Zegaoui, Z., Planchais, S., Cabassa, C., Djebbar, R., Belbachir, O.A., and Carol, P., 2017. Variation in relative water content, proline accumulation and stress gene expression in two cowpea landraces under drought. Australian Journal Plant Physiology 218: 26-34.