Response of Lentil (Lens culinaris L.) Yield and Physiological Traits to Chemical and Bio-phosphorus Fertilizers under Different Irrigation Regimes

Document Type : Scientific - Research


1 tabriz university

2 sari university

3 Tehran

4 tehran


Different studies in semi-arid regions showed that application of phosphorus fertilizers under drought stress conditions increased the crop yield. Most agricultural soils contain larger amount of fixed form of Phosphorus (P) than available P, a considerable part of which has accumulated as a consequence of regular applications of P fertilizers. Certain microorganisms such as phosphate solubilizing bacteria fungi, actinomycetes mostly those associated with the plant rhizosphere are known to convert insoluble inorganic phosphorus into soluble form that could be utilized by the plants. Among them, some phosphate-solubilizing bacteria (PSB) are being used as phosphatic biofertilizers for crop production. Phosphate solubilizing bacteria can solubilize fixed form of P to available form by the secretion of various kinds of organic acids, phosphatase enzyme, growth hormones etc. and increase availability of P to the plants. In this point of view, phosphatic biofertilizer may be used as an alternate option of chemical P fertilizer for lentil cultivation. Therefore, the present study was under taken to evaluate the effects of biofertilizer, chemical phosphorus and integrated application of fertilizers on yield and physiologic characteristics of lentil under drought and normal condition in karaj climate.
Materials and Methods
To evaluate the effect of drought stress on seed yield and physiological traits of lentil cultivar (Ziba) with chemical and bio phosphorus fertilizer, an experiment was conducted in split plot based on randomized complete blocks design CRBD design with four replications in research farm of University of Tehran (Karaj-Iran) during 2014-15. The main factor included three irrigation levels (irrigation after 70, 90 and 130 mm evaporation from open pan class A) and sub treatment were the kind of phosphorus fertilizers (100% super phosphate triple (P); bio-fertilizer; 50% P + bio-fertilizer; no p fertilizer and bio-fertilizer as a check) The bio-fertilizer contained co-inoculation of phosphobacterin (pseudomonas strains 93 & 187) and azotobactor. The characteristics recorded were no. of pods per plant, seed yield, seed protein content (%), relative water content (RWC), proline content and total leaf chlorophyll.
For statistical analysis, analysis of variance (ANOVA) and Duncan’s multiple range test (DMRT) were performed using SAS ver. 9.2 software.
Results and Discussion
Interaction effect of irrigation and type of fertilizer had significant effect on pod number per plant, protein percentage, relative water content, free proline and chlorophyll content. The results indicated that normal irrigation (irrigation after 70 mm evaporation from open pan class A) × integrated phosphorus fertilizer (50% P + inoculation with bio-fertilizer) gave the highest seed yield. Similarly, P and bio-fertilizers application under severe drought stress condition produced higher seed yield compared to other treatments. This can be explained that PB inoculation enhanced the solubilization of phosphates in rhizosphere soil of lentil which stimulated the native Rhizobium and attributed to increase the nodule number and weight ultimately that showed positive effects on other growth and yield attributes of lentil.
However, for the RWC under normal irrigation × bio-fertilizer; for the total leaf chlorophyll under normal irrigation × 100% super phosphate triple; and for the proline content under severe drought stress × 100% super phosphate triple gave the highest values. With drought stress conditions, the leaf RWC and Proline contents reduced, which represented a relationship between these physiological traits and lentil seed yield.
In general, from the view of reducing chemical fertilizer uses to manner the environment-friendly and cost-saving cultivation, application of 50% P + seed inoculation with bio-fertilizer based on the result of this study is recommended.


Adediran, J.A., Taiwo, L.B., Akande, M.O. Sobulo, R.A., and Idowu, O.J. 2004. Application of organic and inorganic fertilizer for sustainable maize and cowpea yield. Nigerian Journal of Plant Nutrition 27: 1163-1181.
Ahmadi Fard, M., Azizi, K., Ismaili, A. Heydari S., and Daraei Mofard, A. 2011. The effects of different fertilization methods on seed yield and components of lentil (lens culinaris) under Khoramabad climatic condition. Iranian Journal of Agricultural Sciences, 4(40): 1-14. (In Persian with English Summary)
Anjum, F., Yaseen, M., Rasul, E., Wahid, A., and Anjum, S. 2003. Water stress in barley (Hordeum vulgare L.). II. Effect on chemical composition and chlorophyll contents. Pakistan Journal of Agricultural Sciences 40: 45-49.
Arnon, I. 1986. Crop Production in Dry Regions. Translated by Koocheki, A., and Alizadeh, A. Published by Mashhad University. Pp. 650.
Arpana, N., Kumar, S.D., and Prasad, T.N. 2002. Effect of seed inoculation, fertility and irrigation on uptake of major nutrients and soil fertility status after harvest of late sown lentil. Journal of Applied Biology 12: 23-26.
Bates, L.S., Waldren R.P., and Teare, I.D. 1973. Rapid determination of free proline for water stress studies. Plant and Soil 39: 205-207.
Efeoglu, B., Ekmekci, Y., and Cicek, N. 2009. Physiological responses of three maize cultivars to drought stress and recovery. South African Journal of Botany 75: 34-42.
Ehteshami, S.M.R., Aghaalikhani, M., Khavazi, K., and Chaichi, M.R. 2007. Effect of Phosphate Solubilizing Microorganisms on quantitative and qualitative characteristics of Maize (Zea mays L) under water deficit stress. Pakistan Journal of Biological Sciences 10 (Suppl 20): 3585-3591.
Fathian, S. 2008. Physiological limitation to safflower photosynthesis under two different moisture regimes. M.Sc. Thesis, Isfahan University of Technology, Iran. (In Persian with English Summary)
Gholami, A., Akbari, A., and Abbasdokht, H. 2015. Study of application of biologic and organic fertilizer on growth and yield characteristics of fennel (Foeniculum vulgare Mill.). Journal of Agroecology 7(2): 215-224.
Gull, M., Hafeez, F.Y., Saleem, M., and Mallik, K.A. 2004. Phosphate uptake and growth promotion of chickpea (Cicer arietinum) by co-inoculation of mineral phosphate solubilizing bacteria and a mixed rhizobial culture. Australian Journal of Agricultural Research 44(6): 623-628.
Jalilian, J., Modarres- Sanavi, A.M., and Sabaghpour, S.H. 2005. Effect of plant density and supplementary irrigation on yield, yield components and protein content of four varieties of chickpea in rain fed condition. Journal of Agricultural Science and Natural Resources 5: 42-51. (In Persian with English Summary)
Jain, L.K., Singh, P., and Balyan, J.K. 2006. Productivity and profitability of chickpea (Cicer arietinum L.) cultivation as influenced by biofertilizers and phosphorus fertilization. Indian Journal of Dryland Agricultural Research Development 21: 82-84.
Kapure, R.M., and Naik, R.M. 2004. Effect of biofertilizers on N, P contents of leaves, available ‘P’ from soil, leghemoglobin and chlorophyll content in chickpea. Journal of Soils and Crops 14: 22-25.
Karimi, K., Bolandnazar, S., and Ashoori, S. 2013. Effect of bio-fertilizer and arbuscular mycorrhizal fungi on yield, growth characteristics and quality of green bean (Phaseolous vulgaris L.). Journal of Sustainable Agriculture and Production Science 23(3): 157-167.
Merah, O. 2001. Potential importance of water status traits for durum wheat improvement under Mediterranean conditions. Journal of Agricultural Research 137: 139-145.
Mohammadpour Vashvayi, R., Glovy, M., Ramrodi, M., and Fakheri, B. 2015. Effect of drought stress and biologic fertilizer incubation on growth, yield and thymus (Thymus vulgare L.) oil composition. Journal of Agroecology 7(2): 237-253.
Nouri, A., Etminan, A., Silva, J.A.T.D., and Mohammadi, R. 2011. Assessment of yield, yield-related traits and drought tolerance of durum wheat varieties (Triticum turjidum var. durum Desf.). Australian Journal of Crop Science 5: 8-16.
Parsa, M., and Bagheri, A. 2008. Pulses. Mashhad University. Mashhad, Iran. 522p. (In Persian)
Puente, M.E., Bashan, Y., Li, C.Y., and Lebsky, V.K. 2004. Microbial populations and activities in the rhizoplane of rock-weathering desert plants. I. Root colonization and weathering of igneous rocks. Plant Biology 6: 629-642.
Rezvani Moghaddam P., Norouzian A., and Seyyedi, S.M. 2015. Evaluation the effects of manure and mycorrhizal inoculation on grain and oil yield of spring safflower cultivars (Carthamus tinctorius L.). Journal of Agroecology 7(3): 331-343. (In Persian with English Summary)
Rodriguez, H., and Fraga, R. 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology Advances 17: 319-339
Roy, R.N., Finck, A., Blair, G.J., and Tandon, H.L.S. 2006. Plant Nutrition for Food Security. FAO Fertilizer and Plant Nutrition Bulletin 16. Food and Agricultural Organization, Rome, p. 348.
Sanchez, F.J., Manzanares, M., Andres, E.F., Ternorio, J.L., Ayerbe, L., and De Andres, E.F. 1998. Turgor maintenance, osmotic adjustment and soluble sugar and praline accumulation in 49 pea cultivars in response to water stress. Field Crop Research 59: 225-235.
Sani, V.K., Bhandari, S.C., and Tarafdar, J.C. 2004. Comparison of crop yield, soil microbial C, N and P, N-fixation, nodulation and Mycorhizal infection in inoculated and non-inoculated sorghum and chickpea crops. Field Crop Research 89: 39-47
Shivakumar, B.G., Balloli, S.S,. and Saraf, C.S. 2004. Effect of sources and levels of phosphorus with and without seed inoculation on the performance of rainfed chickpea (Cicer arietinum L.). Annals of Agricultural Research 25: 320-326.
Somasegaran, P., and Hoben, H.J. 1994. Hand Book for Rhizobia: Methods in Legume-Rhizobium Technology. New York. Springer-Verlag, U.S.A.