Investigation of Biochar Application Effect on Photosynthetic Pigments, Enzymes Activity and Yield of Safflower under Water Stress

Document Type : Research Article

Authors

1 Shiraz University

2 Shiraz university

3 Assistant Professor of Agro-Ecology Department, Faculty of Agriculture and Natural Resources of Darab, Shiraz University, Shiraz

Abstract

Abstract

Introduction

Water stress is one of the main factors in crop growth in arid and semi-arid regions of the world, which limited the grain yield of the safflower. To increase the soil fertility and yield of crops, it is necessary to pay sufficient attention to the use of biomass of organic matter, especially crop residues and its conversion to biochar. This research aims to evaluate the biochar application produced from cotton and wheat residues under late season water stress in the south of Fars province (Darab) on photosynthetic pigments, enzyme activity, yield components, and yield of safflower.

Materials and methods

In order to investigate the biochar application and water stress on the amount of photosynthetic pigments, enzyme activity and yield of safflower, a field experiment was laid out as split plots in the form of randomized complete block design with 3 replications in the 2019-2020 growing season. The experimental factors include the irrigation regime as the main factor in two levels (optimal irrigation and cutting off irrigation after the flowering stage) and secondary factors was fertilizer treatment as sub plot including control (without fertilizer), consumption of 3 tons of biochar from wheat residues per hectare, consumption of 3 tons of biochar from cotton residues per hectare, consumption of 150 kg of urea per hectare + 50 kg of triple superphosphate per hectare, consumption of 112.5 kg of urea per hectare + 37.5 kg of triple superphosphate per hectare, consumption of 112.5 kg of urea + 37.5 kg of triple superphosphate + 3 tons wheat biomass per hectare, and 112.5 kg of urea + 37.5 kg of triple superphosphate + 3 tons of cotton biomass per hectare. In this research, chlorophyl a and b, total chlorophyll, catalase and peroxydase activities, yield components and yield of safflower were calculated.

Results and Discassion

The results of the experiment showed that the measured traits were significantly affected by the irrigation regime and fertilizer treatment. Under cutting off irrigation after the flowering stage, the fertilizer treatment of 112.5 urea + 37.5 phosphate + 3 tons of wheat biochar per hectare increased 55 and 40% chlorophyll a and total chlorophyll compared to control, respectively. Water stress increased the carotenoid content, catalase and peroxidase enzymes. The highest amount of catalase and peroxidase enzyme activity was obtained in the control under cutting off irrigation after the flowering stage, which increased by 48 and 38%, respectively, compared to the control under optimal irrigation. The number of seed per capitule and the number of fertile capitule per plant in the treatment of 112.5 urea + 37.5 phosphate + 3 tons of cotton biochar under water stress conditions, increased by 8.8 and 19.51%, respectively. Also, under water stress, the application 112.5 urea + 37.5 phosphate + 3 tons of cotton biochar, improved and increased the relative content of leaf water, biological yield and grain yield compared to the control by 53, 22 and %34, compared to control, respectively.





Conclusion

Results showed that in both of the irrigation regimes, application of 3 tons of cotton or wheat biochar alone, can increase the biomass, and grain yield compared to control. Overall, application of 112.5 urea + 37.5 phosphate + 3 tons of cotton biochar under water stress conditions, improved the relative water content of the leaves, number of seed per capitule, the number of fertile capitule per plant, and finally biomass and yield of safflower. With respect to water shortage under late season in south parts of Fars province, and in order to decrease the detrimental effects of water stress, farmers can use the biochar of cotton combined with reduced dosage of urea and triple superphosphate fertilizers.

Keywords

Main Subjects


CAPTCHA Image

Articles in Press, Accepted Manuscript
Available Online from 18 April 2023
  • Receive Date: 23 December 2022
  • Revise Date: 26 February 2023
  • Accept Date: 18 April 2023
  • First Publish Date: 18 April 2023