Effect of Organic, Biological and Chemical Fertilizers on Yield, Yield Components and Oil Yield of Black Seed (Nigella sativa L.)

Document Type : Scientific - Research

Authors

1 Department of Agrotechnology, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran

2 Department of Agrotechnology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.

3 Department of Agrotechnology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

4 Department of Soil Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

Abstract

Introduction
 In recent decades, agricultural production has largely relied on the use of chemical inputs, which has led to major environmental problems. Destruction of water and soil resources, deterioration of agricultural biodiversity, air and water pollution by chemical fertilizers and pesticides are only part of the environmental problems caused by common agricultural use of chemical inputs. One of the solutions to this problem is to apply sustainable farming principles in agricultural ecosystems. Soil management is one of the main factors in achieving sustainable agriculture. Therefore, the gradual replacement of chemical fertilizers with organic and biofertilizers will help to meet the nutritional needs of plants, improve the physical, chemical and biological conditions of the soil and prevent the adverse environmental effects of chemical applications. Organic matter has been recognized as one of the nutrients of plant nutrition and fertility due to its constitutive effects on soil physiological and biological properties. Organic fertilizers are the most important source of organic matter in the rhizosphere of plants. Black seed is an annual, dicotyledonous, herbaceous, medicinal plant belonging to the Ranunculaceae family. Environmental conditions and soil fertility are one of the factors affecting grain yield, quantity and quality of essential oil and oil produced in black seed. Since there is not much research on the effect of humic acid and fulvic acid on black seed, therefore, attention to the management of nutritional needs of black seed with emphasis on the use of biological and organic fertilizers in the successful cultivation of this plant is inevitable.
Materials and Methods
In order to evaluate the effects of organic, biological and chemical fertilizers on yield, yield components and oil yield of black seed, a field experiment was conducted at Research Station, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran, during the growing season of 2011-2012. The experimental layout was factorial based on randomized complete block design with three replications. Experimental treatments included all combinations of organic and biological fertilizers factor in four levels (biosulfure + 100 kg.ha-1 sulphor, humic acid (7 kg.ha-1), fulvic acid (7 kg.ha-1) and control) and chemical and animal manure factor in three levels (chemical fertilizer (NPK (80:40:30 kg.ha-1, respectively), animal manure (20 t.ha-1) and control). In order to determine the oil content, 5 grams of grain were randomly selected from the grains harvested from each plot and then oil content was determined by Soxhlet. Analysis of variance (ANOVA) and regression analysis of the data from the experiment and drawing of shapes were performed using SAS and MSTAT software's. Comparisons of means were performed at 1 and 5% probability level using Duncan multiple range test.
Results and Discussion
The Results showed that studied factors and their interactions had significant effect on number of seed per plant, seed weight/plant, seed yield and oil yield. The highest plant height, number of branch per plant, number of follicles per plant, number of seeds per follicles , number of seeds per plant, seed weight per plant, seed yield and oil yield (59 cm, 5.8,7.8,72, 594,1.075g, 2.2 (t.ha-1), 517 (kg.ha-1), respectively) were observed in chemical fertilizer + biosulfur biofertilizer treatment and  the lowest mentioned traits (49 cm, 3.01, 3.92, 39.8, 194, 0.445, 0.935 (t.ha-1), 221 (kg.ha-1) respectively) were observed in control treatment. The highest and lowest harvest indexes were observed in animal fertilizer + humic acid (49%) and control (25.6%) treatments, respectively. The highest and lowest oil percentages were observed in animal fertilizer + humic acid (49%) and control (25.6%) treatments, respectively. The results indicated that the use of humic and fulvic acids, and biosulfur biofertilizer alone or in combination with chemical fertilizers and animal manure improve the quantity and quality of Nigella sativa characteristics.
Conclusion
The results of this study revealed that considering the positive response of the black seed to application of organic and biological fertilizers, applying these fertilizers while reducing the use of chemical fertilizers as well as having no adverse environmental effects is an appropriate method for sustainable production and environmental protection.

Keywords


Ahmadiyan, A., Ghanbari, A., and Gelavi, M., 2009. Effect of animal manure on quantitative and qualitative yield and chemical composition of essential oil in cumin (Cuminum cyminum). Journal of Iranian Field Crop Research 4(2): 207-216. (In Persian with English Summary)
Aghhavani Shajari, M., 2012. Effects of single and combined application of nutrients on quantitative and qualitative indices of coriander (Coriandrum sativum L.). M.Sc. Thesis , Faculty of Agriculture, Ferdowsi University of Mashhad, Iran. (In Persian with English Summary)
Aiken, G.R., McKnight, D.M., Wershaw, R.L., and MacCarthy, P., 1985. Humic Substances in Soil, Sediment and Water. Wiley-Interscience, New York, USA.
Akbarnejad, F., Astaraei, A.R., Fotovat, A., and Nassiri Mahallati, M.,2011. Effect of municipal solid waste compost and sewage sludge on yield and yield components of black Cumin (Nigella sativa L.). Journal of Iranian Field Crop Research 8(5): 767-771.(In Persian with English Summary)
Arun, K.S., 2002. A Handbook of Organic Farming. Pub. Agrobios, India. 626 p.
Alvarez, D., Ludena, P., and Fratos, Y.E., 1992. Correlation and causation among sunflower traits. Proc. 13th. International Sunflower Conference, Pisa. Italy p. 182-204.
Berti, W.R., and Jacobs, I.W., 1996. Chemistry and phytotoxicity of soil trace elements from repeated sewage sludge application. Journal of Environmental Quality 25: 1025-1032.
Chen, Y., and Aviad, T., 1990. Effects of humic substances on plant growth. In: P. MacCarthy et al. (eds.) Humic Substances in Soil and Crop Science: Selected readings. SSSA and ASA, Madison, WI, U.S.A 161-186.
Chen, J., 2006. The combined use of chemical and organic fertilizers and/or biofertilizer for crop growth and soil fertility. International Workshop on Sustained Management of the Soil-rhizosphere System for Efficient Crop Production and Fertilizer Use, Bangkok, Thailand p. 16-20.
Chatterjee, S.K., 2002. Cultivation of medicinal and aromatic plants in India a commercial approach. Proceedings of an International Conference on MAP, Acta Horticulture (ISHS), Springa-Verlag, Berlin 576: 191-202.
Cooper, R.J., Liu, C.H., and Fisher, D.S., 1998. Influence of humic substances on rooting and nutrient content of creeping bentgrass. Crop Science 38: 1639-1644.
Dehghani Meshkani, M.R., Naghdi Badi, H.A., Darzi, M.T., Mehrafarin, A., and Rezazadeh, B.A., 2010. Effect of biological and chemical fertilizers on yield and quality chamomile Shirazi. (Matricaria recutita L.). Journal of Medicinal Plants 2(38): 35-48. (In Persian with English Summary)
EneJi, A.E.R., Islam, P., and Amalu, U.C., 2013. Nitrate retention and physiological adjustment of maize to soil amendment with superabsorbent polymers. Journal of Cleaner Production 52: 474-480.
 Farzaneh, H., 1990. AgroChemistry, (translation). Avaye Nur, Press, Iran. 318 p. (In Persian)
Fallahi, J., Khocheki, A.R., and Rezvani Moghaddam, P., 2008. Investigating the effect of organic fertilizers on quantitative index and the amount of essential oil and chamazulene in chamomile (Matricaria chamomilla). Agricultural Research (Water, Soil and Plant Agricultural) 8(1): 157-168. (In Persian with English Summary)
Forughifar, H. and Purkamayestani, M.A., 2002. Sciences and Management of Soil (vol.1). (Translation). Ferdowsi University of Mashhad, Iran. Press. 467 p. (In Persian)
Ganpat, S., Jshwar, S., and Bahti, D.S., 1992. Response of blond psyllium (Plantago ovata) to irrigation and split application of nitrogen. Indian. Journal of Agronomy 37: 880-881.
Gaur, A.C., Ostwal, K.P., and Mathur, R.S., 1980. Save super phosphate by using phosphate-solubilizing culturesand rock phosphate. Kheti 32: 23-25.
Gilesm J., 2004. Is organic food better for us? Nature, London 428: 796-797.
Garcia, O., 1991. Isolation and characterization of Aciditiobacillus thiooxidans and Aciditiobacillus ferrooxidans from mineral mines. Revista Berasileira de Microbiologia 20: 1-6.
Ghorbani, S., Khazaei, H.R., Kafi, M., and Bannayan Aval, M., 2011. Effects of humic acid application with irrigation water on yield and yield components of corn (Zea mays L.). Journal of Agroecology 1(2): 111-118. (In Persian with English Summary)
Harper, S.M.., Kerven, G.L., Edwards, D.G., and Ostatek-Boczynski, Z., 2000. Characterisation of fulvic and humic acids from leaves of Eucalyptus camaldulensis and from decomposed hay. Soil Biochemical 32: 1331-1336.
Hellal. F.A., Mahfouz, S.A., and Hassan, F.A.S., 2011. Partial substitution of mineral nitrogen fertilizer by bio-fertilizer on (Anethum graveolens L.) plant. Agriculture and Biology Journal of North America 2(4):652-660.
Jones, D.L., and Darrah, P.R., 1996. Re-sorption of organic compounds by roots of Zea mays L. and itsconsequences in the rhizosphere. Plant and Soil 178: 153-160.
Jutur, P.P., and Reddy, A.R., 2007. Isolation, purification and properties of new restriction endonucleases from Bacillus badius and Bacillus lentus. Microbiological Research 162: 378-383.
Kandeel, A.M., Naglaa, S.A.T., and Sadek, A.A., 2002. Effect ofbiofertilizers on the growth, volatile oil yield and chemical composition of Ocimum basilicum L. plant. Annals of Agricultural Science, Ain Shams University, Cairo, 47(1): 351–371.
Kamayestani, N., 2012. Quantitative and qualitative yield anise (Pimpinella anisum) response to fertilizer management. M.Sc. Thesis, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran.(In Persian with English Summary)
Kramer, A.W., Doane, T.A., Horwath, W.R., and Van Kessel, C., 2002. Combining fertilizer and organic inputs to synchronize N supply in alternative cropping system in California. Agriculture Ecosystem and Environmental 91: 233-243.
Khandan, A., 2004. Effects of organic and inorganic fertilizers on yield and yield components of Plantago ovata Forsk.M.Sc. Thesis , Faculty of Agriculture, Ferdowsi University of Mashhad, Iran.(In Persian with English Summary)
Khorramdel, S., Koocheki, A., Nassiri Mahallati, M., and Ghorbani, R.,2011. Effect of biofertilizers on the yield and yield components of black cumin (Nigella sativa L.). Journal of Iranian Field Crop Research 8(5): 758-766. (In Persian with English Summary)
Khorassani, R.,2010. Phosphorus uptake efficiency in corn, sugar beet and groundnut. Journal of Water and Soil 24(1): 188-180. (In Persian with English Summary)
Lotfi, A., Vahabi Sedehi, A.A., Ganbari, A., and Heydari, M., 2009. The effect of deficit irrigation and manure on quantity and quality traits of plantago ovate Forssk. In Sistan region. Iranian Journal of Medicinal and Aromatic Plants 24(4): 506-518. (In Persian with English Summary)
Maccarthy, P., 2001. The principles of humic substances. Soil Science 166:738–751.
Macilwain, C., 2004. Is organic farming better for the environment? Nature 428: 797-798.
Majid, H.R. and Schneiter, A.A., 1987. Yield and quality of semi dwarf and standard height sunflower hybrids grown at five plant populations. Agronomy Journal 79: 681-684.
Michael, K., 2001. Oxidized lignites and extracts from oxidizwd lignites in agriculture. Soil Science: 1-23.
Moradi, R., Nassiri Mahallati, M., Rezvani Moghaddam, P., Lakzian, A. and Ali Nejad, A., 2011. Effect of biological and organic fertilizers on quality and quantity of essential oil of anise (Foeniculum vulgare Mill.). Journal of Horticultural Science 25(1): 25-33.(In Persian with English Summary)
Moradi, R., 2009. Effect of biological and organic fertilizers on quality and quantity of essential oil of anise (Foeniculum vulgare Mill.). M.Sc. Thesis, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran. (In Persian with English Summary)
Mir Hashemi, S.M, Koocheki, A., Parsa, M., and Nassiri Mahallati, M., 2010. Evaluation of growth indices of Ajowan and Fenugreek in pure culture and ntercropping based on organic agriculture. Journal of Iranian Field Crop Research 2(7): 685-693. (In Persian with English Summary)
Natesan, R.S., Kandasamy, S., Thiyageshwari, and Boopathy, P.M., 2007. Influence of lignite humic acid on the micronutrient availability and yield of blackgram in an alfisol. Science World Journal 7: 1198-1206.
Norozpoor, G., and Rezvani Moghaddam, P., 2006.Effect of different irrigation intervals and plant density on oil yield and essences percentage of black cumin (Nigella sativa L.). Iranian Journal of Medicinal and Aromatic Plants 27(3): 134-138. (In Persian with English Summary)
Oehl, F., Sieverding, E., Mäder, P., Duboi, S.D., Ineichen, K., Boller, T. and Wiemken, A., 2004. Impact of long-term conventional and organic farming on the diversity of arbuscular mycorrhizal fungi. Oecologia, 138: 574-583.
Pinamonti, F., 1998. Compost mulch effects on soil fertility, nutritional status and performance of grapevine. Nutrition Cycling Agro-ecosystem 51:239-248.
Pulleman, M.A., Jongmans, J. and Bouma, J., 2003. Effects of organic versus conventional arable farming on soil structure and organic matter dynamics in a marine loam in the Netherlands. Soil Use Management 19:157-165.
Rudresh, D.L., Shivaprakash, M.K., and Prasad, R.D., 2005. Effect of combined application of Rhizobium, phosphate solubilizing bacterium and Trichoderma spp. on growth, nutrient uptake and yield of chickpea (Ciceraritenium L.). Applied Soil Ecology 28: 139-146.
Rezazadeh, H., Khrasani, S.K., and Haghighi, R.S.A., 2012. Effects of humic acid on decrease of phosphorus usage in forage maize var. KSC704 (Zea mays L.). Australian Jurnal of Agricultural Engineering 3: 34-38.
Rezapor, A., Heydari, M., Galavi, M., and Ramrodi, M., 2011. Effect of water stress and different amounts of sulfur fertilizer on grian yield, grain yield components and osmotic adjustment in )Nigella sativa L.). Journal Iranian of Medicinal and Aromatic Plants Research 27(3): 384-396. ( In Persian with English Summary)
Rahimzadeh, S., Sohrabi,Y., Heidari, Gh.R., Eivazi, A.R., and Hoseini, T., 2011. Effect of bio and chemical fertilizer s on yield and quality of dragonhead (Dracocephalum moldavica L.). Iranian Journal of Medicinal and Aromatic Plants 27(1): 81-96. (In Persian with English Summary)
Saeidnejad, A.H. and Rezvani Moghaddam, P.,2011. Investigation the effect of compost, vermicompost, cow and sheep manures on yield, yield components and essence percentage of cumin (Cuminum cyminum L.). Journal of Horticulture Science 2(24): 142-148. (In Persian with English Summary)
Salimpur,S., Khavazi, K., Nadian, H.A., and Besharati, H., 2010. Effect of rock phosphate with sulfur and micro-organisms on yield and chemical composition. Journal of Soil Research 24(1): 9-19. (In Persian with English Summary).
Samavat, S., and Malakooti, M., 2006. Important use of organic acid (humic and fulvic) for increase quantity and quality agriculture productions. Water and Soil Researchers Technical 463: 1-13.
Shaalan, M.N., 2005. Influence of biofertilizers and chicken manure on growth, yield and seeds quality of (Nigella sativa L.) plants. Egyptian Journal of Agricultural Research 83:811-828.
Sharafi, S., Abbas Dokht, H., Chaeichi, M.R., and Ghasemi, S., 2010. The effect of thiobacillus inoculation and type of nitrogen fertilization on yield and yield components (Brassica napus L.) fall cultivars. Iranian Journal of Field Crop Science 41(3): 459-468. (In Persian with English Summary)
Tahami Zarandi, M., Rezvani Moghaddam, P., and Jahan, M., 2011. Comparison the effect of organic and chemical fertilizers on yield and essential oil percentage of basil (Ocimum basilicum L.). Journal of Agroecology 2(1): 70-82. (In Persian with English Summary)
Taylor, G., and Cooper, L., 2004. Humic acid: The root to healthy plant growth. California State Science Fair.
Tejada, M., Gonzalez, J.L., García-Martínez, A.M., and Parrado, J., 2008. Effects of different green manures on soil biological properties and maize yield. Bioresource Technology 99: 1758-1767.
Turgut, I., Bilgili, U., Duman, A., and Acikgoz, E., 2005. Effect of green manuring on the yield of sweet corn. Agronomy Sustainable Development 25:1-5.
Turkmen, O., Demir, S., Sensoy, S., and Dursun, A., 2005. Effect of arbuscular mycorrhizal fungus and humi acid on the seedling development and nutrient content of pepper grown under saline soil conditions. Journal of Biological Sciences 5: 565-574.
Yazdani Biuki, R., Khazaie, H.R., Rezvani Moghaddam, P., and Astaraei, A., 2011. Effects of animal manures and chemical fertilizer on quantitative and qualitative characteristics of milk thistle plant (Silybum marianum). Journal of Iranian Field Crop Research 8(5): 735-746. (In Persian with English Summary)
Younesian, A., 2011. Sustainable management of nutrition in fennel cultivation (Foeniculum vulgare Mill). M.Sc. Thesis, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran.(In Persian with English Summary)
Zargari, A., 1998. Medicinal plants. University of Tehran, Press. 951 p. (In Persian)
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