The Effects of Biological, Chemical Fertilizers and Humic Acid on some Microbial Parameters, Elements and Oil Quantity and Quality of Catnip (Nepeta cataria L.)

Document Type : Scientific - Research

Authors

1 Department of Horticulture Science, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

2 Department of Soil Science, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

Abstract

Introduction
Catnip (Nepeta cataria L.) is one of the most important medicinal plants belonged to the Lamiaceae family that referred to considerable pharmacological, antimicrobial, antibacterial and pesticides activities of its essential oil in most of the literatures. The harmful effects of chemical fertilizers on the environment and human health and lack of soil organic matter in arid and semi-arid regions have resulted in an increase in the use of chemical fertilizers in these areas.Excessive use of these fertilizers has led to serious environmental pollutions during the last decades. With respect to the hazards of chemical fertilizers and the importance of medicinal plants, researchers have recently considered the application of biofertilizers and organic materials. According to literatures, the application of biofertilizers and organic fertilizers can be recommended as a proper alternation for the improvement of the quantity and quality of essential oil in Catnip.
Material and Methods
This research was conducted as factorial layout based on a randomized complete block design (RCBD) with three replications at the research farm of the faculty of agriculture, Shahid Chamran University of Ahvaz, Iran. The first factor was included fertilizer treatments in five levels (Control (C), Azetobarvar-1 (Az), Phosphatbarvar-2 (Ph), Combination of Az and Ph (M), chemical fertilizer (Ch) included 150 kg.ha-1 granular urea (46% N) and 100 Kg.ha-1 triple superphosphate (46% p < sub>2O5)) and the second factor was comprised humic acid in two levels (0 and 20 Kg.ha-1). The studied traits in this research included soil microbial respiration and biomass carbon and soil organic matter contents, leaf nitrogen and phosphorus amounts, number and size of secretory trichomes, essential oil content, yield and composition. Leaf nitrogen and phosphorus concentrations were measured at the beginning of reproductive stage by Kjeldahl method and colorimetric method using a UV-2100-Unico spectrophotometer, respectively. Moreover, the soil microbial respiration and soil biomass carbon were determined by alkali absorption and the fumigated extraction, respectively, and soil organic matter was estimated by the modified Walkly and Blackʼs methods. Furthermore, the number and size of secretory trichomes was registered using Binocular microscope and essential oil was extracted by Clevenger-type apparatus and analyzed using GC and GC-MS at full bloom stage.
Results and Discussion
According to the results, the sole application of fertilizer treatment was positively affected on soil microbial respiration and biomass carbon, leaf nitrogen and phosphorus contents. The number and size of secretory trichomes, essential oil content and yield and essential oil composition were significantly influenced when fertilizer treatments and humic acid were applied simultaneously. The highest amount of soil microbial respiration as well as biomass carbon was detected in M and the lowest was observed in control. The application of Ch and humic acid was significantly improved the soil organic matter. However, no significant difference was found between these treatments and biofertilizers treatments. The lowest amount of soil organic matter was detected in control. The maximum content of leaf nitrogen element was observed in plant treated by Ch and minimum was registered in control. The highest level of leaf phosphorus element, the size of secretory trichomes and essential oil content were obtained when Ph was applied while there was no significant difference between this biofertilizer and Ch and M. The maximum number of secretory trichomes and essential oil yield was detected in plant treated with M. The most amounts of isomers 1 and 2 of nepetalactone and caryophyllene oxide was observed in Az, Ch and control, respectively, and least was in control, Ph and Ch, respectively. The highest level of trans- caryophyllene and (E,E)–Farnesene  was obtained in the oil of plant treated with M+ and Ch+ and lowest detected in Az+ and Ch-.
 Conclusion
According to the results, to improve the quantity and quality of Catnip essential oil, the application of biological fertilizers instead of chemical fertilizers is recommended and further research is also required to investigate both the effects of applying humic acid alone and in combination with biofertilizers on some traits.

Keywords


Adams, R.P., 2007. Identification of Essential Oil Components by Gas Chromatography/Mass Spectroscopy, Fourth ed. Allured Publishing Corporation, Illiois.
Alijani, M., Amini Dehgji, M., Malboei, M.A., Zahedi, M., and Modarres Sanavi, S.A.M., 2012. Effect of phosphorus fertilizer combined with organic fertilizer, phosphate barvar-2 on yield, oil content and chamazulene Chamomile German (Matricaria recutita L.). Iranian Journal of Medicinal and Aromatic Plants 27: 450-459. (In Persian with English Summary)
Amoaghaei, R., and Mostageran, A., 2008. Plant-bacteria symbiosis assistance systems. Isfahan University, Iran. (In Persian).
Anderson, T.H., 2003. Microbial eco-physiological indicators to asses soil quality. Agriculture, Ecosystems and Environment 98: 285-293.
Bakhshandeh Larimi, S., Shakiba, M.R., Dabbagh Mohammadinasab, A., and Moghaddam Vahed, M., 2014. Changes in nitrogen and chlorophyll density and leaf area of Sweet Basil (Ocimum basilicum L.) affected by biofertilizer and nitrogen application. International Journal of Biosciences 9: 256-265.
Barajas Aceves, M., 2005. Comparison of different microbial biomass and activity measurement methods in metal- contaminated soil. Journal Bioresource Technology 96: 1405-1414.
Bingham, F.T., and Bartels, J.M., 1996. Methods of Soil Analysis. Vol. 1, Part 3. Chemical methods. Soil Science Society of America, Madison, Wisconsin, USA. pp. 752-758.
Boveiri Dehsheikh, P., Mahmoodi Sourestani, M., Enayatizami, N., and Zolfaghari, M., 2017. The study on the effect of biological and chemical fertilizers and humic acid on the growth, physiological characteristics and essential oil content of catnip (Nepeta cataria L.). Journal of Plant Production Research 24: 61-76.
Chen, Y., and Ariad, T., 1990. Effects of humic substances in plant growth. Soil Science Society of America, pp. 161-186.
Faraji Mehmani, A., Esmaeilpour, B., Sefidkon, F., Abbaszadeh, B., Khavazi, K., and Ghanbari, A., 2015. Effects of biofertilizers on growth criteria, quantitative and qualitative yield of summer savory (Satureja hortensis L.). Journal of Agroecology 6: 870-879. (In Persian with English Summary)
Farahani, A., and Maddani, H., 2014. Evaluate the usefulness of humic acid organic matter in comparison to chemical fertilizer and manure and their combination in summer savory (Satureja hortensis L.). New Finding in Agriculture 8: 323-337. (In Persian with English Summary)
Fatma, A.G., Lobna, A.M., and Osman, N.M., 2008. Effect of compost and biofertilizers on growth, yield and essential oil of sweet marjoram (Majorana hortensis) Plant. International Journal of Agriculture and Biology 10: 381–387.
Gohary, A.E.E., Sherbeny, S.E.E. and Ghazal, G.M.E.M., 2014. Evaluation of essential oil and monoterpenes of peppermint (Mentha piperita L.) under humic acid with foliar nutrition. Journal of Materials and Environmental Science 5: 1885-1890.
Hosseinzadah, F., Satei, A., and Ramezanpour, M.R., 2011. Effects of mycorhiza and plant growth promoting rhizobacteria on growth, nutrients uptake and physiological characteristics in Calendula officinalis L. Middle-East Journal of Scientific Research 8: 947-953.
Johnson, J.W., and Wilrinson, R.E., 1992.Wheat growth response of cultivars to H+ concentration. Plant and Soil 146: 55-59.
Kaboli Farshchi, H., Azizi, M., Nemeti, H., and Roshan-Sourestani,V., 2015. Effect of potassium sulphate and humic acid on growth, yield and essential oil content in Hypericum perforatum L. Journal of Horticultural Science 29: 518-527. (In Persian with English Summary)
Khalid, K.A., Omer, E.A., El Gendy, A.G., and Hussein, M.S., 2015. Impact of organic compost and humic acid on essential oil composition of sweet fennel (Foeniculum vulgare var. Dulce) under sandy soil conditions in Egypt. World Journal of Pharmaceutical Sciences 3: 160-166.
Khazaie, H.R., Eyshi Rezaie, E., and Eyshi Rezaie, M., 2011. Application times and concentration of humic acid impact on aboveground biomass and oil production of hyssop (Hyssopus officinalis). Journal of Medicinal Plants Research 5: 5148-5154.
Loomis, W.D., and Corteau, R., 1972. Essential oil biosynthesis. Recent Advances in Phytochemistry 6: 147-185.
Maisonneuve, S.A., 1975. European Pharmacopoeia. Sainte-Ruffine.
Pourhadi, M., 2011. Effect of biofertilizers on yield and essential oil of peppermint (Mentha piperita L.). Journal of Herbal Drugs 2: 137-148. (In Persian with English Summary)
Rezaee Chianeh, I., Pirzad, A., and Fargami, A., 2015. The effect of bacteria suppliers nitrogen, phosphorus and sulfur on seed yield and essential oil of cumin (Cuminum cyminum L.). Journal of Agricultural Science and Sustainable Production 24: 71-83. (In Persian with English Summary)
Sharma, A.K., 2002. Biofertilizers for sustainable agriculture. Agrobios, India.
Suschke, U., Geiss. H.K., and Reichling, J., 2006. Antibacterial activity of the essential oils of catnip (Nepeta cataria L.) and lemon balm (Melissa officinalis L.) against clinical isolates from the respiratory tract. Planta Medica 72(11): 27.
Taherabadi, S., Neemati, N.B., Pazoki, A., and Naderi Boroujerdi, G., 2015. Application of biological fertilizer to reducing the need for chemical fertilizers in hyssop (Hyssopus officinalis L.).Agronomic Research in Semi Desert Regions 11: 107-116. (In Persian with English Summary)
Tikhonov, V.V., Yakushev A.V., Zavgorodnyaya, Y.A., Byzov, B.A., and Demin, V.V., 2010. Effects of humic acids on the growth of bacteria. Eurasia Soil Science 43: 305-313.
Tucker, A.O., and Tucker. S.S., 1988. Catnip and the catnip response. Economic Botany 42: 214-231.
Walkly, A., and Black, I.A., 1934. An examination of the degtjare of method for determination soil organic matter and a proposed modification of the chromic acid titration method. Soil Science 37: 29-38.
Weisany, V., Rahimzadeh, S., Sohrabi, Y., 2012. Effect of biofertilizers on morphological, physiological characteristic and essential oil content in basil (Ocimum basilicum L.). Iranian Journal of Medicinal and Aromatic Plants 28: 73-87.
Yoonesy, A.R., Sadeghian Motahar, S.Y., Sajedi, N.A., and Naderi Boroujerdi, G.R., 2014. Effects of biological and chemical fertilizers (nitrogen and phosphorus) on yield and oil content of peppermint plant under the climatically conditions of Arak. New Finding in Agriculture 8: 275-290. (In Persian with English Summary)
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  • Receive Date: 08 April 2019
  • Revise Date: 18 September 2019
  • Accept Date: 06 October 2019
  • First Publish Date: 27 November 2020