Effect of Application of Biofertilizers and Water Treatments on Yield, Yield Components and Swelling Index of Isabgol (Plantago ovata Forssk.)

Document Type : Scientific - Research


Agricultural Research Institute, Zabol University, Zabol, Iran


Isabgol belongs to the plantaginaceae family. It is known for its gentle laxative, cooling and diuretic properties. Some recent researches have shown that they may be effective in lowering cholesterol and controlling certain types of diabetes. Water is the limiting factor for biomass production in many countries around the world, particularly in arid and semi-arid areas. On the other hand, drought has been a serious problem in Iran in recent years. Drought stress reduces the biomass of many crops and medicinal plants as well as decrease of the concentration of nutrients in plants. Several scientists have shown that a plant under stress will produce secondary metabolites that may influence its medicinal properties. Water is one of the most important environmental factors that have a significant influence on the growth and active ingredient of medicinal plants. Water shortages cause serious damage in plant growth and development. During drought and salinity absorption will decrease due to the high concentration of salts in the root zone and increasing the osmotic potential of the soil nutrients. Therefore, a decrease in the soil moisture reduces rate and amount of nutrient absorption by plants. Organic fertilizers under drought stress mitigate impact of drought stress by increasing proline, soluble sugars and absorption of potassium and phosphorus and increase crop yield (Rahimzade, 2009). Bio-fertilizers are inputs containing microorganism which are capable of mobilizing nutritive elements from complex and non-usable form to simple and usable form through biological processes. Also, bio-fertilizers can be substituted and an ideal candidate for commercial fertilizers in sustainable agriculture. The present investigation aims to study the use of deficit irrigation and different bio-fertilizer treatments in an appropriate manner, to achieve the highest quantitative and qualitative yield of isabgol.
Materials and Methods
A field experiment was conducted based on split-plot in randomized complete blocks design with three replications at Darmian city, South Khorasan in 2014-2015 growth season. The main-plots were levels of drought stress including 40, 60, 80 of field capacity (FC) and sub-plots were different bio-fertilizers including nitroxin, phosphate barvar 2, combination (nitroxin + phosphate barvar 2) and control (spray with water). In this experiment traits measured including plant height, number of tiller per plant, number of spike per plant, number of seed per spike, thousand–seed weight, seed yield, turgid index, mucilage percentage and mucilage yield.
Results and Discussion
The results indicated that the number of tiller and spike per plant and thousand-seed weight were significant (p≤0.01) under drought stress and bio-fertilizer. The highest number of seeds per spike was obtained from 80% field capacity with nitroxin application and the lowest was obtained from 40% FC and non-application fertilizer. By increasing drought stress, the number of seeds per spike decreased. Interaction of drought stress and bio-fertilizer had significant effect on plant height, number of seed per spike, seed yield, swelling index and mucilage percentage. The highest 1000-seed weight was obtained from 80% FC, which did not have significant difference with 60% FC. The highest numbers of seed per spike and seed yield were obtained from 80% FC along with nitroxin application and the lowest of them were obtained from 40% FC and no fertilizer (control), which increased by 47.4 and 54.3 percent, respectively. The maximum mucilage percentage was achieved from 80% FC along with phosphate barvar 2 application, although not significant across other fertilizer treatments.
In general, for achievement of maximum isabgol yield, inoculate seed with bio-fertilizer and providing plants water requirements would be appropriate.


Aghazadeh-Khalkhali, D., Mehrafarin, A., Abdossi, V., and Naghdi Badi, H. 2015. Mucilage and seed yield of psyllium (Plantago psyllium L.) in response to foliar application of nano-iron and potassium chelate fertilizer. Journal of Medicinal Plants 4(56): 23-34. (In Persian with English Summary)
Ashraf, M., and Foolad, M.R. 2007. Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany 59: 206-216.
Ashrafi, V., and Talebi, M. 2012. Improve drought resistance in crops, the first national conference on sustainable agricultural development and a healthy environment. Hamedan, Islamic Azad University, Hamedan. (In Persian).
Baher, Z.F., Mirza, M., Ghobani, M., and Rezaii, M.B. 2002. The Influence of water stresses on plant height, herbal and essential oil yield and composition in Satureja hortensis L. Flavour and Fragrance Journal 17: 275-277.
Basudehradum, B.D., Bisha, S., and Manhendrapol, S. 1989. Indian medicinal plants. Today and Tomarrow’s Publication.
Bhagat, N.R. 1980. Studies on variation and association among seed yield and some component traits in Plantago ovata Forsk. Crop Improvement 7: 60-63.
Blumenta, M.A., Goldberg, A., and Brinkman, J. 2000. Herbal Medicine: Expanded Commission E Monographs. Integrative Media Communications Pub., London.
Forouzandeh, M., Mirshekari, S., and Shiri, Y. 2014. Effect of seeds inoculation by plant growth promoting bacteria on seedling growth of five wheat cultivars under greenhouse experiment. Indian Journal of Fundamental Applied Life Science 4(4): 2929-2935.
Ghasemi Siani, E., Fallah, S., and Tadayyon, A. 2011. Study on yield and seed quality of Plantago ovata Forssk. under different nitrogen treatments and deficit irrigation. Iranian Journal of Medicinal and Aromatic Plants 27(3): 517-528. (In Persian with English Summary)
Gliessman, S.R. 2007. Field and Laboratory Investigations in Agroeclogy. CRC Press, Taylor and Francis Grop.
Gupta, G., Parihar, S.S., Ahirwar, N.K., Snehi, S.K., and Singh, V. 2015. Plant growth promoting rhizobacteria (PGPR): Current and future prospects for development of sustainable agriculture. Journal of Microbial and Biochemical Technology 7: 96-102.
Han, H.S., Supanjani, D., and Lee, K.D. 2006. Effect of coin calculation with phosphate and potassium solubilizing bacteria on mineral uptake and growth of pepper and cucumber. Plant, Soil and Environment 52: 130-136.
Koocheki, A., Tabrizi, L., and Nassiri Mahallati, M. 2007. The effect of irrigation intervals and manure on quantitative and qualitative characteristics of Plantago ovata and Plantago psyllium. Asian Journal of Plant Science 6(8): 1229-1234.
Krishna, A., Patil, C.R., Raghavendra, S.M., and Jakati, M.D. 2008. Effect of bio-fertilizers on seed germination and seedling quality of medicinal plants. Karnataka Journal of Agricultural and Science 21: 588-590.
Kundan, R., Pant, G., Jadon, N., and Agrawal, P.K. 2015. Plant growth promoting rhizobacteria: Mechanism and current prospective. Journal of Fertilizers and Pesticides 6(2): 1-9.
Larcher, W. 1995. Physiological Plant Ecology. Third edition. Berlin springer– Verlag.
Lebaschy, M.H., and Sharifi Ashoorabadi, A. 2004. Growth indices of some medicinal plant under different water stresses. Iranian Journal of Medicinal and Aromatic Plants 20(3): 249-261. (In Persian with English Summary)
Lotfi, A., Vahabi Sedehi, A.A., Ganbari, A., and Heydari, M. 2009. The effect of deficit irrigation and manure on quantity and quality traits of plantago ovata Forssk. in Sistan region. Iranian Journal of Medicinal and Aromatic Plants 24(4): 506-518. (In Persian with English Summary)
Mandal, K., Saravanan, R., and Maiti, S. 2008. Effect of different levels of N, P and K on downy mildew (Peronospora plantaginis L.) and seed yield of Plantago ovata. Crop Protection 27(6): 988-995.
Mousavi Nik, M. 2012. Effect of drought stress and sulphur fertilizer on quantity and quality yield of psyllium (Plantago ovata L.) in Baluchestan. Journal of Agroecology 4(2): 170-182. (In Persian with English Summary)
Naghdi Badi, H., Dastpak, A., and Ziai, S.A. 2004. A review of psyllium plant. Journal of Medicinal Plants 3(9): 1-14. (In Persian with English Summary)
Omidbaigi, R. 2005. Production and Processing of Medicinal Plants. Beh-Nashr Publication, Mashhad, Vol. 2, Pp: 438. (In Persian)
Patra, D.D., Anwar, M., Singh, S., Prasad, A., and Singh, D.V. 1999. Aromatic and medicinal plants for salt and moisture stress conditions. Recent advances in management of arid ecosystem. Proceeding of a Symposium Held in India, 26-28 March, 347-350.
Pouryousef, M., Chaichi, M.R., Mazaheri, D., Fakhretabataii, M., and Ashraf Jafari, A. 2007. Effect of different soil fertilizing system on seed and mucilage yield and seed P content of isabgol (Plantago aovata Forsk). Asian Journal of Plant Science 6(7): 1088-1092.
Rahimzade, S. 2009. Effect of bio-fertilizers on yield and quality of dragonhead (Dracocephalum moldavica L.) under field conditions. MSc dissertation, Faculty of Agriculture, University of Kurdistan, Iran. (In Persian with English Summary)
Ramroudi, M., Galavi, M., Siahsar, B.A., and Allahdoo, M. 2011a. Effect of micronutrient and irrigation deficit on yield and yield components of isabgol (Plantago ovata Forsk) using multivariate analysis. Journal of Food and Agricultural Environment 9: 247-251.
Ramroudi, M., Keykha Jaleh, M., Galavi, M., Seghatolesalmi, S.M.J., and Baradaran, R. 2011b. The effect of various micronutrient foliar applications and irrigation regimes on quantitative and qualitative yield of isabgol (Plantago aovata Forsk). Journal of Agroecology 3(2): 219-226. (In Persian with English Summary)
Samadi, M., and Sepehri, A. 2012. Effect of plant density and biofertilizers on yield and yield components of isabgol (Plantago ovata). In: Proceedings of the 12th Iranian Congress of Agronomy and Plant Breeding, 5-7 Sept. 2012. Islamic Azad University, Karaj, Iran. (In Persian with English Summary)
Sepehri, A. and Samadi, M. 2015. The effect of integrated chemical and biological fertilizers on growth indices and mucilage yield of isabgol (plantago ovata Forsek.) across different plant densities. Iranian Journal of Field Crop Research 13(3): 485-495. (In Persian with English Summary)
Sharma P.K., and Koul, A.K. 1986. Mucilage in seeds Plantago ovata and its wild allies. Journal of Ethnopharmachology 17: 289-295.
Sreevalli, Y., Baskaran, K., Chandrashekara, R., Kuikkarni, R., SuShil Hasan, S., Samresh, D., Kukre, J., Ashok, A., Sharmr Singh, K., Srikant, S., and Rakesh, T. 2001. Preliminary observations on the effect of irrigation frequency and genotypes on yield and alkaloid concentration in periwinkle. Journal of Medicinal and Aromatic Plant Science 22(35): 356-358.
Yadav, R.D., Keshwa, G.L., and Yadav, S.S. 2002. Effect of integrated use of FYM, urea and Sulphur on growth and yield of isabgol (Plantago ovata). Journal of Medicinal and Aromatic Plant Science 25: 668-671.
Yousefpoor, Z., and Yadavi, A.R. 2014. Effect of biological and chemical fertilizers of nitrogen and phosphorus on quantitative and qualitative yield of sunflower. Sustainable Agriculture and Production Science 24(1): 95-112. (In Persian with English Summary)
Zahir, A.Z., Arshad, M., and Frankenberger, W.F. 2004. Plant growth promoting rhizobacteria: Applications and per-spectives in agriculture. Agronomy Advances 81: 97-168.
Zhenixi, I., Wang, P., and Zhang, T. 2008. Effects of types and application rates of nitrogen fertilizer on the development and nitrogen utilization of summer maize. Frontiers of Agriculture in China 1: 44-49