Application of Eco-Friendly Inputs on Cultivation of Iranian Ox-Tongue (Echium amoenum Fisch. & Mey.) in Conditions of Mashhad

Document Type : Scientific - Research

Authors

1 Department of Agronomy, Faculty of Agriculture, University of Gonabad,Gonabad, Iran.

2 Agrotechnology Department, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

Abstract

Introduction
In recent years, the effect of ecological inputs on soil properties and plant growth characteristics has received renewed attention. Although the utilization of mineral fertilizers could be viewed as the best solution in terms of plant productivity, this approach is often inefficient in the long-term in tropical ecosystems due to the limited ability of low-activity clay soils to retain nutrients. Biofertilizer is a substance which contains living microorganisms which, as applied to seed, plant surfaces, or soil, colonizes the rhizosphere or the interior of the plant and promotes growth by increasing the supply or availability of primary nutrients to the host plant. Biofertilizers add nutrients through the natural processes of nitrogen fixation, solubilizing phosphorous and stimulating plant growth through the synthesis of growth-promoting substances. Biofertilizers can be expected to reduce the use of chemical fertilizers and pesticides. Humic substances are a group of heterogeneous molecules that are bonded together by weak forces; therefore, they have a high chemical stability. This material included of 65 to 80 percent of total soil organic matter. Humic acid influences soil fertility through its effect on the water-holding capacity of the soil. Humic acids make important contributions to soil stability and soil fertility leading to exceptional plant growth and micronutrient uptake. Mycorrhiza arbuscular fungi are other coexist microorganisms that improves soil fertility, nutrients cycling and agroecosystem health. Mycorrhizal fungi are the most abundant organisms in agricultural soils. Many researchers have pointed to the positive roles of mycorrhizal fungi on plants growth characteristics. Echium amoenum is a wild perennial herb and known in Iran as Ox-Tongue. It is one of the important medicinal plants in Iranian traditional medicine. Petals of Iranian Ox-Tongue have been advocated for a variety of effects such as demulcent, anti-inflammatory and analgesic, especially for common cold, anxiolytic, sedative and other psychiatric symptoms including obsession in folk medicine of Iran. Despite of many researches on the effect of organic acids and biofertilizers on different crops, information on the effects of these factors for many medicinal plants is scarce, therefore, in this study effect of eco-friendly inputs in cultivation of Echium amoenum in conditions of Mashhad was studied.   
Materials and Methods
In order to evaluate organic acids, plant growth rhizobacteria and different species of mycorrhiza on yield and yield components of Iranian Ox-Tongue (Echium amoenum), a split plots experiment in time based on RCBD design with three replications was conducted in 2011-2014 growing seasons, in Ferdowsi University of Mashhad, Iran. Seven different types of soil amendments and biofertilizers concluded humic acid, fulvic acid, Nitroxin® (containing Azotobacter spp. And Azospirillum spp.), Biophosphorous® (containing Bacillus sp. and Pseudomonas sp.), Biosulfur® (containing Thiobacillus spp.), Mycorrhiza (Glomus mosseae), Mycorrhiza (Glomus intraradices), and no fertilizer as control and agronomic years (2 and 3 agronomic years) assigned to main and sub plots, respectively.
Result and Discussion
The results showed that the highest dry flower yield obtained in humic acid treatment. Humic acid and fulvic acid increased seed weight per plant 38 and 25% and seed number per plant 19 and 17% compared to control, respectively. Fulvic acid, nitroxin, biophosphorous, biosulfur, mycorrhiza (Glomus mosseae) and mycorrhiza (Glomus intraradices) increased dry flower yield 27, 20, 4, 26, 30 and 28% compared to control, respectively. The highest flower and seed harvest index obtained in mycorrhiza (Glomus intraradices) and humic acid treatments, respectively. It seems that different species of mycorrhiza improved morphological characteristics and yield of Echium amoenum due to provide better conditions to absorption and transportation of nutrient to the plant. It has been reported that organic acids provided favorable conditions for plant growth and development through improvement of physical, chemical and biological properties of the soil, therefore, it can be concluded that improvement  of most of studied traits in the present study were due to use of organic acids.
Conclusion
In general, the findings of this research showed that application of ecological inputs, while increasing yield and yield components of Iranian Ox-Tongue, can reduce dependence on chemical inputs and their environmental risks and can be used to develop sustainable agriculture and protect the health of the environment.

Keywords


Abdul Jaleel, C., Manivannan, P., Sankar, B., Kishorekumar, A., Gopi, R., Somasundaram, R., and Panneerselvam, R. 2007. Pseudomonas fluorescens enhances biomass yield in Catharanthus roseus under water deficit stress. Colloids and Surfaces B: Biointerfaces 60:7-11.
Agha Alikhani, M., Iranpour, A., and Naghdi Badi, H. 2013. Changes in agronomical and phytochemical yield of purple coneflower (Echinaceae purpurea (L.) Moench) under urea and three biofertilizers application. The Journal of Medical Research 12: 121-136.
Azzaz, N.A., Hassan, E., and Hamad, E.H. 2009. The chemical constituent and vegetative and yielding characteristics of fennel plants treated with organic and bio-fertilizer instead of mineral fertilizer. Australian Journal of Basic and Applied Sciences 3: 579-587.
Baghdadi, H., Daneshian, J., Yousefi, M., Alimohammadi, M., and Kheybari, M. 2012. Influence of cattle manure and mycorrhiza fungi on vegetative growth of pumpkin under water deficit conditions. International Journal of Agriculture and Crop Sciences 4: 1362-1365.
Benabdellah, K., Abbas, Y., Abourouh, M., Aroca, R., and Azcon, R. 2011. Influence of two bacterial isolates from degraded and non-degraded soils and arbuscular mycorrhizae fungi isolated from semi-arid zone on the growth of Trifolium repens under drought conditions: Mechanisms related to bacterial effectiveness. European Journal of Soil Biology 47(5): 303-309.
Bera, A.K., Pramanik, K., and Panda, D. 2013. Response of biofertilizers and homo-brassinolide on growth, relative water content and yield of lentil (Lens culinaris Medik.). Journal of Crop and Weed 9: 84-90.
Chen, J. 2006. The combined use of chemical and organic fertilizers and/or biofertilizer for crop growth and soil fertility. International Workshop on Sustained Management of the Soil-Rhizosphere System for Efficient crop Production and Fertilizer Use. October, 16-20. Thailand.
Copetta, A., Lingua, G., and Berta, G. 2006. Effects of three AM fungi on growth, distribution of glandular hairs, and essential oil peoduction in Ocimum basilicum L. var. Genovese. Mycorrhiza 16: 485-494.
Darzi, M.T., Haj Seyyed Mohammad Hadi, M.R., and Rejali, F. 2012. Effect of cattle manure and biofertilizers on biomass yield, seed yield and oil content of Coriandrum sativum. Iranian Journal of Medicinal and Aromatic Plants 11: 77-91. (Persian with English Summary)
Egamberdiyeva, D. 2005. Plant-growth-promoting rhizobacteria isolated from a Calcisol in a semi-arid region of Uzbekistan: Biochemical characterization and effectiveness. Journal of the Plant Nutrition and Soil Science 168: 94-99.
El-Baz, S.M., Abbas, E.E., and Mostafa, R.A.I.A. 2012. Effect of sowing dates and humic acid on productivity and infection with rot diseases of some soybean cultivars cultivated in new reclaimed soil. International Journal of Agricultural Research 7: 345-357.
Gharib, F.A., Moussa, L.A., and Massoud, O.N. 2008. Effect of compost and bio-fertilizers on growth, yield and essential oil of sweet marjoram (Majorana hortensis) plant. International Journal of Agricultura and Biology 10: 381-387.
Ghasemi, A. 2009. Medicinal and Aromatic Plants, Identifying and Studying their Effects. Publications of Islamic Azad University, Shahrkord, Iran. (In Persian with English Summary)
Ghasemi, K., Fallah, S., Raeesi, F., and Heidari, M. 2013. Effect of urea fertilizer and biofertilizers on quantitative and qualitiative yield of Plantago ovata Forsk.). Journal of Plant Production Research 101-116. (In Persian with English Summary)
Gupta, M.L., Prasad, A., Ram, M., and Kumar, S. 2002. Effect of the vescular-arbuscular mycorrhizal (VAM) fungus Glomus fasciculatum on the essential oil yield related characters and nutrient acquisition in the crops of different cultivars of menthol mint (Mentha arvensis) under field conditions. Bioresource Technology 81: 77-79.
Hashemzadeh, F., Mirshekari, B., Khoe, F.R., Yarnia, M., and Tarinejad, A. 2013. Effect of bio and chemical fertilizers on seed yield and its components of dill (Anethum graveolens). Journal of Medicinal Plants Research 7: 111-117.
Heidari, M.R., Azad, E.M., and Mehrabani, M. 2006. Evaluation of the analgesic effect of Echium amoenum Fisch & C.A. Mey. extract in mice: Possible mechanism involved. Journal of Ethnopharmacology 103: 345-349.
Heidari, M., and Karami, V. 2014. Effects of different mycorrhiza species on grain yield, nutrient uptake and oil content of sunflower under water stress. Journal of the Saudi Society of Agricultural Sciences 13: 9-13.
Hoseini Mazinani, S.M., and Hadipoor, A. 2014. Improve the quality and quantity of Calendula officinalis L. via biofertilizers application. Journal of Medicinal Plants 13: 83-93. (In Persian with English Summary)
Kapoor, R., Giri, B., and Mukerji, K.G. 2002. Glomus macrocarpum: potential bioinoculant to improve essential oil quality and concentration in dill (Anethum gravolens L.) and carum (Trachyspermum ammi (Linn.) Sprague). World Journal of Microbiology and Biotechnology 18: 459-463.
Kapoor, R., Giri, B., and Mukerji, K.G. 2004. Improved growth and essential oil yield and quality in (Foenicudum vulgare mill.) on mycorrhizal inoculation supplemented with P- fertilizer. Bioresource Technology 93: 307-311.
Kapoor, R., Chaudhary, V., and Bhatnagar, A.K. 2007. Effects of arbuscular mycorrhiza and phosphorus application on artemisinin concentration in Artemisia annua L. Mycrrhiza 17: 581- 587.
Karr, M. 2001. Oxidized ignites and extracts from oxidized ignites in agriculture. Soil Science 1-23.
Khazaee, H.R., and Banayan, M. 2011. Application of use time and amount of humic acid on biomass and oil content of Hyssopus officinalis. Iranian Journal of Medicinal and Aromatic Plants 5: 25-35. (In Persian with English Summary)
Kızılkaya, R. 2008. Yield response and nitrogen concentrations of spring wheat (Triticum aestivum) inoculated with Azotobacter chroococcum strains. Ecological Engineering 33: 150-156.
Mahfouz, S.A., and Sharaf-Eldin, M.A. 2007. Effect of mineral vs. biofertilizer on growth, yield, and essential oil content of fennel (Foeniculum vulgare Mill.). International Agrophysics 21: 361-366.
Manaffee, W.F., and Kloepper, J.W. 1994. Application of plant growth promoting rhizobacteria in sustainable agriculture. In: “Soil biota management in sustainable farming systems”. CSIRO, Publication. East Melbourne, Australia p. 23-31.
Marulanda, A., Porcel, R., Barea, J.M., and Azcon, R. 2007. Drought tolerance and antioxidant activities in laender plants colonized by native drought-tolerant of drought-sensitive Glomus species. Microbial Ecology 54: 543-552.
Massoud, O.N., Afifi, M.M.I., El-Akshar, Y.S., and El-Sayed, G.A.M. 2013. Impact of biofertilizers and humic acid on the growth and yield of wheat grown in reclaimed sandy soil. Research Journal of Agriculture and Biological 9: 104-113.
Mohammadipour, E., Golchin, A., Mohammadi, J., Negahdar, N., and Zarchini, M. 2012. Effect of humic acid on yield and quality of marigold (Calendula officinalis L.). Annals of Biological Research 3: 5095-5098.
Mondal, M.M.A., Malek, M.A., Sattar, M.A., Puteh, A.B., Rafii, M.Y., and Ismail, M.R. 2013. Response of biofertilizer and urea on growth and yield in mungbean. Legume Research 36: 448-452.
Natesan, R., Kandasamy, S., Thiyageshwari, S., and Boopathy, P.M. 2007. Influence of lignite humic acid on the micronutrient availability and yield of blackgram in an alfisol. Scientific World Journal 7: 1198-1206.
Nooriyan Soroor, E., Rouzbehan, Y., and Alipour, D. 2013. Effect of Echium amoenum extract on the growth rate and fermentation parameters of Mehraban lambs. Animal Feed Science and Technology 184: 49-57.
Pal, K.K., Tilak, V.B.R., Saxena, A.K., Dey, R., and Singh, C.S. 2001. Suppression of maize root diseases caused by Macrophomia phaseolina, Fusarium moniliforme and Fusarium graminaerum caused by plant growth promoting rhizobacteria. Microbiological Research 156: 209-223.
Rajendran, K., and. Devaraj, P. 2004. Biomass and nutrient distribution and their return of Casuarina equisetifolia inoculated with biofertilizers in farm land. Biomass and Bioenergy 26: 235-249.
Richter, J., Stutzer, M., and Schellenberg, I. 2005. Effects of mycorrhization on the essential oil content and composition of aroma components of marjoram (Marjorana hortensis), thyme (Thymus vulgaris L.) and caraway (Carum carvi L.). 36th International Symposium on Essential Oils, 4-7 September, Budapest, Hungary.
Rillig, M.C., and Mummey, D.L. 2006. Mycorrhizas and soil structure. New Phytologist 171: 41-53.
Roesti, D., Gaur, R., Johri, B.N., Imfeld, G., Sharma, S., Kawaljeet, K., and Aragno, M. 2006. Plant growth stage, fertilizer management and bio-inoculation of arbuscular mycorrhizal fungi and plant growth promoting rhizobacteria affect the rhizobacterial community structure in rain-fed wheat fields. Soil Biology and Biochemistry 38: 1111-1120.
Sahin, F., Cakmakci, R., and Kantar, F. 2004. Sugar beet and barley yields in relation to inoculation with N2-fixing and phosphate solubilizing bacteria. Plant and Soil 265: 123-129.
Samavat, S., and Malakooti, M. 2005. Production necessity and use of organic acids to increase the quality and quantity of agricultural products. Publications of Sana, Tehran, Iran. (In Persian with English Summary)
Sanches Govin, E., Rodrigues Gonzales, H., and Carballo Guerra, C. 2005. Ifluencia de los abonos organicos y biofertilizantes en la calidad de las especies medicinales calendula officinalis L. y Matricaria recutita L. Revista Cubana de Plantas Medicinales 10: 1-5.
Sarir, M.S., Sharif, M., Ahmed, Z., and Akhlaq, M. 2005. Influence of different levels of humic acid application by various methods on the yield and yield components of maize. Sarhad. Jouranl of Agriculture 21: 75-81.
Sayyah, M., Boostani, H., Pakseresht, S., and Malaieri, A. 2009. Efficacy of aqueous extract of Echium amoenum in treatment of obsessive-compulsive disorder. Progress in Neuro-Psychopharmacology and Biological Psychiatry 33: 1513-1516.
Shaalan, M.N. 2005. Effect of compost and different sources of biofertilizers, on borage plants (Borago officinalis). Egypt Journal of Agriculture Research 83: 271-279.
Shabahang, J., Khorramdel, S., and Gheshm, R. 2013. Effect of mycorrhiza on yield and yield components of Foeniculum vulgare and Carum copticum L. affected by nitrogen amounts. Journal of Agroecology 5: 289-298. (In Persian with English Summary)
Sidari, M., Attina, E., Francioso, O., Tugnoli, V., and Nardi, S. 2006. Biological activity of humic substances is related to their chemical structure. Soil Science Society of America Journal 71: 75-85.
Sirrenberg, A., Gobel, C., Grond, S., Czempinski, N., Ratzinger, A., Karlovsky, P., Santos, P., Feussner, I., and Pawlowski, K. 2007. Piriformospora indica affects plant growth by auxin production. Journal of Plant Physiology 131: 581-589.
Smith, S.E, GIaninazzi-Pearson, V., Koide, R., and Cairney, J.W.G. 1994. Nutrient Transport in Mycorrhizas: Structure, Physiology and Consequences for Efficiency of the Symbiosis. In: Robson, A.D., Abbott, L.K. and Malajczuk, N. (Eds) Management of Mycorrhizas in Agriculture, Horticulture and Forestry. Kluwer Academic Publishers, Dordrecht p. 103-113.
Thomas, P. 1984. Canola Grower Manual. Canola Concil of Canada Publication. 14 pages.
Verlinden, G., Pycke, B., Mertens, J., Debersaques, F., Verheyen, K., Baert, G., Bries, J., and Haesaert, G. 2009. Application of humic substances results in consistent increases in crop yield and nutrient uptake. Journal of Plant Nutrition 32: 1407-1426.
Vessey, J.K. 2003. Plant growth promoting rhizobacteria as biofertilizer. Plant and Soil 255: 571-586.
Vestberg, M., Saari, k., Kukkonen, S., and Hurme, T. 2005. Mycotrophy of crops in rotation and soil amendment with peat influence the abundance and effectiveness of indigenous arbuscular mycorrhizal fungi in field soil. Mycorrhiza 15: 447-458.
Zhu, C.X., Song, B.F., and Xu, W.H. 2010. Arbuscular mycorrhizae improves low temperature stress in maize via alterations in host water status and photosynthesis. Plant and Soil 331: 129-137.
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