The Role of Trichoderma and Enterobacter Inoculation on Improving Wheat Yield in Different Levels of Phosphorus Fertilizer

Document Type : Scientific - Research


1 Tarbiat Modares University, Tehran, Iran

2 Department of Agronomy and Plant Breeding, Genetic and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, Sari

3 Sari Agricultural Sciences and Natural Resources University


Regarding to the high cost and environmental pollution due to the overuse of chemical fertilizers, an adoptable option for farmers are biofertilizers to increase crop productivity per unit area. Bio-fertilizers are essential components of organic farming that play a vital role in maintaining long-term fertility and sustainability of the soil by their ability to provide micro- and macro-nutrients and also to convert insoluble phosphorus (P) into available forms to plants. Phosphorus as an essential nutrient is often fixed by most soils and is unavailable to the plants. Therefore, application of the plant growth promoting rhizobacteria (PGPR) such as phosphate solubilizing bacteria (PSB) can be proposed as an efficient solution to provide P for plants. These microorganisms by staying on the plant roots surface through production of organic acids, vitamins and other growth promoting materials could increase grain yield (GY) in many crops. In addition, Trichoderma spp. fungus species symbiosis with plant roots and root growth stimulation could control plant pathogens by production of antibiotics, induced systemic resistance and improve plant health that are known as other plant growth promoting microorganisms. It has been reported that Trichoderma species have an ability to solubilizing soil insoluble phosphate which resulted in improving the soil fertility and plant growth as well. Wheat (Triticum aestivumL.) is an important source of human foods which its production is highly depending on the application of chemical fertilizers. Therefore, to reduce the economic and environmental problems, this study aimed to investigate the effect of Trichodermahamatum fungi along with Enterobacter sp. as a PSB on yield and yield components of wheat (cv. ‘Milan’) in different levels of phosphorus fertilizer (TSP).
Materials and Methods
A field experiment was conducted in Mazandaran province (Sari city, located at 36°38' N, 53°32' E and 13.5 m asl) in 2014-15. The experiment was carried out in a factorial split plot arrangement based on a randomized complete blocks design with three replications.Three levels of triple super phosphate (TSP; zero, 50 and 100 kg ha-1) were used as the main plot and both fungal (inoculation with Trichodermahamatum and uninoculated control) and phosphate solubilizing bacteria (PSB) inoculations (inoculation with Enterobacter sp. and uninoculated control), were served as the sub-plots. In this experiment, the traits related to yield and yield components including number of spikes per square meter, grain number and grain weight per spike, straw yield and biological yield, grain yield (t.ha-1), harvest index and 1000-grain weight (g) were measured. Finally, data analysis was performed using SAS 9.1 and means were compared by the least significant difference (LSD) test at a 5% level of probability.
Results and Discussion
The results indicated that the effect of both Trichodermahamatum and Enterobacter sp. were statistically significant on yield and yield components of wheat. GY increased from 2.66 to 3.45 (29.69%), 3.19 to 3.79 (18.80%) and 3.34 to 4.39 t.ha-1 (31.43%) for 0, 50 and 100 kg.ha-1 of TSP, respectively, when Trichodermahamatumwas used separately. In contrast, single PSB inoculation increased GY from 2.66 to 3.37 (21.06%), 3.19 to 3.46 (8.46%) and 3.34 to 5.10 t.ha-1 (52.69%) when applied 0, 50 and 100 kg.ha-1 of TSP, respectively. Simultaneous application of these microorganisms significantly increased GY from 2.66 to 3.78 (42.10%), from 3.19 to 3.85 (20.68%) and from 3.34 to 4.48 t.ha-1 (34.13%) for 0, 50 and 100 kg.ha-1 of TSP, respectively.
The result of this study clearly indicate that presence of both Trichodermahamatum and Enterobacter sp. along with different levels of TSP can increase yield and yield components of wheat as compared to the application of TSP alone (without Trichodermahamatum and Enterobacter sp.).
This study was supported by a grant from the Genetics and Agricultural Biotechnology Institute of Tabarestan and Sari Agricultural Sciences and Natural Resources University, Iran. The authors thank Mr. FarzinGoran for his assistance in the field experiment.


Abd-El-Khair, H., Khalifa, R.K.M., and Haggag, K.H.E. 2010. Effect of Trichoderma species on damping off diseases incidence, some plant enzymes activity and nutritional status of bean plants. Journal of American Science 6: 486-497.‏
Ahangar, L., Babaezad, V., Ranjbar, G.A., Najafi Zarrini, H., and Biabani, A. 2015. Expression profile of defense-related genes in susceptible and resistant wheat cultivars in response to powdery mildew infection. Modern Genetics Journal 10: 33-46. (In Persian with English Summary)
Arzanesh, M.H., and Faraji, A. 2015. Effect of plant growth promoting bacteria (Azospirillum sp.) on physiology and yield of two oilseed rapes. Journal of Management System 2: 159-171. (In Persian with English Summary)
Bakhshandeh, E., Rahimian, H., Pirdashti, H., and Nematzadeh, G.A. 2014. Phosphate solubilization potential and modeling of stress tolerance of rhizobacteria from rice paddy soil in northern Iran. World Journal of Microbiology and Biotechnology 30: 2437-2447.
Bakhshandeh, E., Rahimian, H., Pirdashti, H., and Nematzadeh, G.A. 2015. Evaluation of phosphate‐solubilizing bacteria on the growth and grain yield of rice (Oryza sativa L.) cropped in northern Iran. Journal of Applied Microbiology 119: 1371-1382.
Ebrahimi-Chamani, H., Yasari, E., and Pirdashti, H.A. 2014. Effects of phosphate solubilizing bacteria and phosphorous levels on rice (Oryza sativa L.). Agricultural Advances 3: 56-66.‏
Harman, G.E., Howell, C.R., Viterbo, A., Chet, I., and Lorito, M. 2004. Trichoderma species-opportunistic, avirulent plant symbionts. Nature Reviews Microbiology 2: 43-56.
Hassan, T.U., and Bano, A. 2015. Role of carrier-based biofertilizer in reclamation of saline soil and wheat growth. Archives of Agronomy and Soil Science 61: 1719-1731.
Hossain, M.B., and Sattar, M.A. 2014. Effect of inorganic phosphorus fertilizer and inoculants on yield and phosphorus use efficiency of wheat. Journal of Environmental Science and Natural Resources 7: 75-79.‏
Kandula, D.R.W., Alizadeh, H., Teixiera, C.S.P., Gale, D., Stewart, A., and Hampton, J.G. 2015. Trichoderma bio-inoculant improves seedling emergence, plant growth and seed yield of Camelina sativa (L.) Crantz. New Zealand Plant Protection 68: 160-165.‏
Kapri, A., and Tewari, L. 2010. Phosphate solubilization potential and phosphatase activity of rhizospheric Trichoderma spp. Brazilian Journal of Microbiology 41: 787-795.‏
Lavakush, Y.J., Verma, J.P., Jaiswal, D.K., and Kumar, A. 2014. Evaluation of PGPR and different concentration of phosphorus level on plant growth, yield and nutrient content of rice (Oryza sativa L.). Ecological Engineering 62: 123-128.‏
Mahdi, S.S., Hassan, G.I., Samoon, S.A., Rather, H.A., Dar, S.A., and Zehra, B. 2010. Bio-fertilizers in organic agriculture. Journal of Phytology 2: 42-54.
Malik, A.U., Malghani, A.L., and Hussain, F. 2012. Growth and yield response of wheat (Triticum aestivum L.) to phosphobacterial inoculation. Russian Agricultural Sciences 38: 11-13.‏
Mirshekari, B., Hokmalipour, S., Sharifi, R.S., Farahvash, F., and Ebadi-Khazine-Gadim, A. 2012. Effect of seed biopriming with plant growth promoting rhizobacteria (PGPR) on yield and dry matter accumulation of spring barley (Hordeum vulgare L.) at various levels of nitrogen and phosphorus fertilizers. Journal of Food Agriculture and Environment 10: 314-320.‏
Moghadassi, R. 2009. An strategy to meet food security (based on determined goals in agricultural section). Islamic Parliament Research Center, Tehran. (In Persian)
Mohammadi, K., Ghalavand, A., Aghaalikhani, M., and Rokhzadi, A. 2011a. Effect of different methods of soil fertility increasing via application of organic, chemical and biological fertilizers on grain yield and quality of canola (Brassica napus L.). Journal of Agroecology 3: 298-308. (In Persian with English Summary).
Mohammadi, K., Ghalavand, A., Aghaalikhani, M., Heidari, G., and Sohrabi, Y. 2011b. Introducing a sustainable soil fertility system for chickpea (Cicer arietinum L.). African Journal of Biotechnology 10: 6011-6020.‏
Moubarak, M.Y., and Abdel-Monaim, M.F. 2011. Effect of bio-control agents on yield, yield components and root rot control in two wheat cultivars at New Valley region, Egypt. Journal of Cereals and Oilseeds 2: 77-87.‏
Noumavo, P.A., Kochoni, E., Didagbe, Y.O., Adjanohoun, A., Allagbe, M., Sikirou, R., Gachomo, E.W., Kotchoni, S.O., and Baba-Moussa, L. 2013. Effect of different plant growth promoting Rhizobacteria on maize seed germination and seedling development.‏ American Journal of Plant Sciences 4: 1013-1021.
Rudresh, D.L., Shivaprakash, M.K., and Prasad, R.D. 2005. Tricalcium phosphate solubilizing abilities of Trichoderma spp. in relation to P uptake and growth and yield parameters of chickpea (Cicer arietinum L.). Canadian Journal of Microbiology 51: 217-222.‏
Saber, Z., Pirdashti, H., Esmaeili, M., Abbasian, A., and Heidarzadeh, A. 2012. Response of wheat growth parameters to co-inoculation of plant growth promoting rhizobacteria (PGPR) and different levels of inorganic nitrogen and phosphorus. World Applied Sciences Journal 16: 213-219.‏
Sengupta, C., Bhosale, A., and Malusare, S. 2015. Effect of plant growth promoting rhizobacteria on seed germination and seedling development of Zea mays. International Journal of Research in Advent Technology Special Issue, National Conference on Advances and Chalenges in Green Technology, Pune, India, 13-14 February p. 32-40.
Seyed Sharifi, R., and Khavazi, K., 2012. Effect of seed inoculation with plant growth promoting rhizobacteria (PGPR) on germination components and seedling growth of corn (Zea mays L.). Journal of Agroecology 3: 506-513. (In Persian with English Summary)
Shaharoona, B., Naveed, M., Arshad, M., and Zahir, Z.A. 2008. Fertilizer-dependent efficiency of Pseudomonads for improving growth, yield, and nutrient use efficiency of wheat (Triticum aestivum L.). Applied Journal of Microbiology and Biotechnology 79: 147-155.‏
Shahid, M., Hameed, S., Imran, A., Ali, S., and Van Elsas, J.D. 2012. Root colonization and growth promotion of sunflower (Helianthus annuus L.) by phosphate solubilizing Enterobacter sp. Fs-11. World Journal of Microbiology and Biotechnology 28: 2749-2758.‏
Sharma, P., Patel, A.N., Saini, M.K., and Deep, S. 2012. Field demonstration of Trichoderma harzianum as a plant growth promoter in wheat (Triticum aestivum L.). Journal of Agricultural Science 4: 65-73.‏
Shoresh, M., Harman, G.E., and Mastouri, F., 2010. Induced systemic resistance and plant responses to fungal biocontrol agents. Annual Review of Phytopathology 48: 21-43.
Singh, V., Singh, P.N., Yadav, R.L., Awasthi, S.K., Joshi, B.B., Singh, R.K., Lal, R.J., and Duttamajumder, S.K. 2010. Increasing the efficacy of Trichoderma harzianum for nutrient uptake and control of red rot in sugarcane. Journal of Horticulture and Forestry 2: 66-71.‏
Tripathi, P., Singh, P.C., Mishra, A., Tripathi, R.D., and Nautiyal, C.S. 2015. Trichoderma inoculation augments grain amino acids and mineral nutrients by modulating arsenic speciation and accumulation in chickpea (Cicer arietinum L.). Ecotoxicology and Environmental Safety 117: 72-80.‏
Verma, J.P., Yadav, J., Tiwari, K.N., and Kumar, A., 2013. Effect of indigenous Mesorhizobium spp. and plant growth promoting rhizobacteria on yields and nutrients uptake of chickpea (Cicer arietinum L.) under sustainable agriculture. Ecological Engineering 51: 282-286.‏
Verma, M., Brar, S.K., Tyagi, R.D., Surampalli, R.Y., and Valèro, J.R. 2007. Antagonistic fungi, Trichoderma spp.: Panoply of biological control. Biochemical Engineering Journal 37: 1-20.
Vinale, F., Sivasithamparam, K., Ghisalberti, E.L., Marra, R., Woo, S.L., and Lorito, M. 2008. Trichoderma-plant-pathogen interactions. Soil Biology and Biochemistry 40: 1-10.
Yadav, A., Yadav, K., and Aggarwal, A. 2015. Impact of arbuscular mycorrhizal fungi with Trichoderma viride and Pseudomonas fluorescens on growth, yield and oil content in Helianthus annuus (L.). Journal of Essential Oil Bearing Plants 18: 444-454.
Zaidi, A., Saghir Khan, M.D., and Amil, M.D. 2003. Interactive effect of rhizotrophic microorganisms on yield and nutrient uptake of chickpea (Cicer arietinum L.). European Journal of Agronomy 19: 15-21.‏
Zhu, F., Qu, L., Hong, X., and Sun, X. 2011. Isolation and characterization of a phosphate-solubilizing halophilic bacterium Kushneria sp. YCWA18 from Daqiao Saltern on the coast of Yellow Sea of China. Evidence-Based Complementary and Alternative Medicine 2011: 1-6.‏