An evaluation of the impact of the endophyte fungus Piriformospora indica on some traits of barley (Hordeum vulgare L.) in drought stress

Document Type : Scientific - Research

Authors

Mehdi Ghabouli ¹

Farajollah Shahriary ¹

Mozhgan Sepehri ²

Hasan Marashi ¹

Ghasem Hosseini Salekdeh ³

¹ Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

² Department Soil Science, Faculty of Agriculture, Isfahan University of Technology, Isfahan, Iran

³ Agricultural Biotechnology Research Institute of Iran, Karaj, Iran

10.22067/jag.v3i3.13558

Abstract

Microbial endophytes, which make one of the most important classes of soil microorganisms, induce genetic, physiological and ecological alterations in their host plant and thus increase its yield and enable its cultivation in saline or dry soils or in climates facing biotic and abiotic stresses. The endophyte fungus Piriformospora indica exhibits a high effect in plant growth and increased resistance against environmental tensions like drought and salinity, as well as against phytopathogens. This work was intended to study the potential of P. indica in enhancing growth and elevating drought resistance in barley (Hordeum vulgare L.) during 2010. A greenhouse trial of completely randomized design with two fungal treatments (inoculated vs. non-inoculated) and three drought levels (F.C., 50% F.C. and 25% F.C.) with four repeats was conducted in greenhouse of Agricultural Biotechnology Research Institute (Isfahan). The results indicate that the fungus P. indica has accompanied biomass increments of both shoot and root parts in the inoculated plant compared to the control, as in inoculated plant, total shoot dry weight and root dry weight were increased by 39 and 46 percent, respectively. Also, in stress conditions RWC in inoculated plant was greater. In addition to the growth increasing activity, the effective role of the fungus in enhancing barley growth and yield under drought conditions (especially at the 25% F.C. level) is evident. According to the results, and to the fact that the fungus can be cultured on artificial (host-plant-free) growth medium, this fungus can be contemplated as making a growth stimulating agent and in producing biological fertilizer for use in crops; and it might take a significant role toward a sustainable agriculture. The application of this fungus can also be beneficial in increasing growth and production of crops such as barley and wheat under the dry conditions widely encountered in Iran.

Keywords

environmental stress

Inoculation

Mycorrihzal fungi

Sustainable agriculture

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