Nodulations, nitrogen fixation and growth characteristics of chickpea under metribuzin herbicide application

Document Type : Scientific - Research



In order to study the effects of herbicide rates of metribuzin on nodulations and growth characteristics of chickpea, a series of experiments were carried out at research greenhouses of Ferdowsi University of Mashhad during 2009 in a randomized complete block design with four replications. Studied factors were: different doses of metribuzin herbicide (10- 25- 50- 75- 100- 125 percent of recommended dose) in autoclaved soil and two doses of 25 and 50 percent in un-autoclaved soil. The highest dry weight of root and shoot, height and leaf number was in no herbicide and inoculated control and lowest rate gained at 100 and 125 percent of herbicide doses. The highest root/shoot rate gained at herbicide doses of 100 and 125 percent. The changes trend of nodule number and dry weight per plant were significant (p


Abasi, R., Arab, C.M., Alizadeh, H.M., Moazen ghamsari, B., 1384. Evolution effect of herbicide on amount of
nitrogen fixation on pulse. First Proceeding of Pulse, Mashhad. (In Persian with English summary).
2- Abd-Alla, M.H., Omar, S.A. Karanzha, S., 2000. The impact of pesticides on arbuscular mycorrhizal and nitrogenfixing
symbioses in legumes. Appl. Soil. Ecol. 14, 191-200.
3- Aamil, M., 2002. Effect of agrochemicals on soil microflora and some important cereal and legume crops. Ph.D.
Thesis, Fac. Agric. Aligarh Muslim Univ: Aligarh., India.
4- Anderson, A., Baldock, J.A., Rogers, S.L., Bellotti, W., Gill, G., 2004. Influence of chlorsulfuron on rhizobial
growth, nodulae formation, and nitrogen fixation with chickpea. Aust. J. Agr. Res. 55, 1059-1070.
5- Bagheri, A., Zand, A., Parsa, M., 1376. Pulse problems. Jihad-e-Daneshgahi of Mashhad, Iran. (In Persian with
English summary).
6- Bhuian, M.H.A., Khanam, M.F., Ahmed, M.S., 2008. Effect of Rhizobium Inoculation on nodulation and yield of
Chickpea in calcareous soil. Bangladesh J. Agr. Res. 33, 549-554.
7- Bordeleau, I.M., Prevost D., 1994. Nodulation and nitrogen fixation in extreme environment. Plant. Soil. 161, 115-
8- Datta A., Sindel, B.M., Kristiansen, P., Jessop, R.S., Felton W.L., 2009. Effect of isoxaflutole on the growth,
nodulation and nitrogen fixation of chickpea (Cicer arietinum L.). Crop Prot. 28, 923-927.
44 نشریه بوم شناسی کشاورزی، جلد 1، شماره 2، سال 1388
9- Eberbach, P.L. Douglas, L.A., 1989. Herbicide effects on the growth and nodulation potential of Rhizobium trifoli
with Trifolium subterraneum L. Plant. Soil. 15, 23-119
10- Hernandez, A., Gracia Plazaola, J.I., Becerril, J.M., 1999. Glyphosate effects on Phenolic metabolism of nodulated
soybean (Glycine max L. Merr.). J. Agr. Food Chem. 47: 2920–2925.
11- Islam R., 1982. Lentil Experimental News Service, Canada, 9, 23-24.
12- Iswaran, V., Marwah, T.S., 1980. Amodified rapid Kjeldahl Method for determination of total nitrogen in
agricultural And biological materials. Geobios. 7, 281–282.
13- Kafi, M., Mahdavi damghani, A., 1379. Mechanism of tolerance to environmental stress in plant. Ferdowsi Univ.
Mashhad., Iran. (In Persian with English summary).
14- Norris, G.R.F., Phillips, D.A., 1978. Effect of bentazon, a hill reaction inhibitor, on symbiotic sitrogen-fixing
capability and apparent photosynthesis. Plant. Physiol. 63, 213-215.
15- Mallik, M.A.B., Tesfai, K., 1985. Pesticide affect on soybean rhizobia symbiosis. Plant. Soil. 85, 33–41.
16- Parsa, M., Bagheri, A., 1387. Pulse. Jihad-e-Daneshgahi of Mashhad. (In Persian with English summary).
17- Rennie, R.J., Dubetz S., 1984. Effect of fungicides and herbicides on nodulation and N-fixation in soybean files
lacking indigenous Rhizobium japonicum. Agron. J. 76, 451-454.
18- Singh, G., Wright, D., 1999. Effects of herbicides on nodulation, symbiotic nitrogen fixation, growth and yield of
pea (Pisum sativum). Agr. Sci. 133, 21-30.
19- Singh, G., Wright, D., 2002. In vitro studies on the effects of herbicides on the growth of rhizobia. Lett. Appl.
Microbiol. 35:12-16.
20- Sprout, S.L., Nelson L.M., Germida, J.J., 1992. Influence of metribuzin on the Rhizobium leguminosarum – lentil
(Lens culinaris) symbiosis. Can. J. Microbiol. 38, 343-349.
21- Sandhu, P.S., Dhingra, K.K., Bhandari, S.C., Gupta, R.P., 1991. Effect of hand-hoeing and application of
herbicides on nodulation, nodule activity and grain yield of Lens culinaris. Plant. Soil. 135, 293-296.
22- Saghir Khan, M., Almas Zaidi, M., Qamar Rizvi, P., 2006. Biotoxic effects of herbicides on growth, nodulation,
nitrogenase activity, and seed production in chickpeas. Commun. Soil. Sci. Plan. 37, 11, 1783-1793.
23- Zaidi, A., Saghir Khan, M.D., Amil, M.D., 2003. Interactive effect of rhizotrophic microorganisms on yield and
nutrient uptake of chickpea (Cicer arietinum L.). Eur. J. Agron. 19, 15–21.
24- Walley, F., Taylor, A., Lupwayi, N., 2006. Herbicide residues & effects on nitrogen fixation in pulse crops.
Presented at Farm Tech. 2006, Herbicide Effects on Pulse Crop Nodulation and Nitrogen Fixation. p, 52-55.