Effect of Drought Stress on Growth and Morphological Characteristics of Two Garlic (Allium sativum L.) Ecotypes in Different Planting Densities

Document Type : Scientific - Research

Authors

• Shiva Akbari ¹
• Mohammad Kafi ²
• Shahram Rezvan Beidokhti ¹

¹ Department of Agronomy, Faculty of Agriculture, Damghan Branch, Islamic Damghan University, Damghan, Iran

² Department of Agronomy, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

10.22067/jag.v9i2.62141

Abstract

Introduction
Plants may be exposed to various stresses and water deficit is the most important limiting factor of growth and yield in many parts of the world and Iran. Stress induced growth decrement can be because of cell development decrease due to decrement of turgor pressure and meiosis and photosynthesis decrease due to stomata closure. Determination of desired planting density is one of the success factors of plant growth and production. Garlic (Allium sativum) has been an important medicinal plant over centuries in human life. According to the importance of medicinal plants and studying the effects of drought stress on them, the goal of this research is to investigate the effect of drought stress and planting density on growth and morphological characteristics of two ecotypes of garlic and determining the preferable ecotype and density from the perspective of these traits.
Materials and methods
This experiment was performed in 2012 in a farm in south east of Semnan. It was conducted on a split-plot factorial arrangement based on randomized complete blocks design with three replications. Three levels of drought stress with 60, 80 and 100 percent of crop evapotranspiration (ETc) were the main plot factors and factorial combination of three planting density (30, 40 and 50 plants.m-2) and two ecotypes of Tabas and Toroud were the levels of sub plot factors. To estimate water requirement of garlic, daily measured meteorology parameters of Semnan synoptic station were used and water requirement was calculated based on FAO-56 instructions. From mid-January, the sampling of leaf area, bulb and leaf fresh and dry weight was started with destructive method every other week and continued until middle of Jun. three plant were selected randomly from each plot in each turn. From middle of May, height and number of leaves were measured. Leaf area measurement was done by leaf area meter (Delta-T). To estimate growth indices, dry weight of aerial and underground organs and leaf area from measured samples of treatments were used.
Results and Discussion
The results showed that the highest value of maximum leaf area index (LAI), maximum total dry matter accumulation (TDM), maximum crop growth rate (CGR) and maximum of net assimilation rate (NAR) were observed at 100% ETc with the value of 5.537, 387.53 gr.m-2, 10.47 gr m-2day-1 and 4.92 gr.m-2leaf.day-1 respectively; and by applying the irrigation treatment of 80% ETc these values decreased to 3.745, 262.60 gr.m-2, 6.31 gr.m-2day-1 and 3.71 gr.m-2leaf.day-1 respectively. Drought stress can decrease cell development and division and plant photosynthesis, and thus, it can decrease leaf area index and consequently decrease light absorption, photosynthetic area, dry matter and crop growth rate. Difference between ecotypes in terms of number of leaves, maximum total dry matter accumulation and maximum crop growth rate was significant. Maximum crop growth rate (CGR) in Toroud ecotype was higher than Tabas ecotype but number of leaves and maximum concentration of total dry matter (TDM) in Tabas ecotype were higher than corresponding values in Toruod ecotype. The effect of planting density on maximum leaf area index (LAI), maximum total dry matter (TDM) and maximum net assimilation rate (NAR) was significant. The highest value of maximum leaf area index (5.017) and maximum total dry matter (358.57 g.m-2) concentration were obtained from 50 plant.m-2 density. The highest value of maximum net assimilation rate (4.61) was obtained from 30 plant.m-2 density. It could be because of having leaves exposed to more light and less shading.
Conclusion
Applying drought stress at the irrigation treatment of 80% ETc decreased studied growth characteristics of garlic. Therefore, it is recommended that garlic should be avoided from facing drought stress and its water requirement must be met as much as possible. In general, under drought stress, two studied ecotypes did not have any preference related to the studied growth characteristics. Higher planting density, due to higher active photosynthetic area over unit area, increased the dry matter in unit area.

Keywords

• Crop Growth Rate
• Dry matter
• Evapotranspiration
• Leaf Area Index
• Plant height

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References

Ahmadzadeh Ghavidel, R., Asadi, G.A., Naseri Pouryazdi, M.T., Ghorbani, R., and Khorramdel, S. 2016. Effects of plant density and cow manure levels on growth criteria of bean (Phaseolus vulgaris L.) cultivars under Mashhad climatic conditions. Journal of Agroecology 8(2): 296-317. (In Persian with English Summary)

Akbari, S., Kafi, M., and Rezvan Beidokhti, S. 2016. The effects of drought stress on yield, yield components and antioxidant of two Garlic (Allium sativum L.) ecotypes with different planting densities. Journal of Agroecology 8(1): 95-106. (In Persian with English Summary)

Allen, R.G, Periera, L.S., Raes, D., and Smith, M. 1998. Crop evapotranspiration guidelines for computing crop water requirements. FAO Irrigation and Drainage Paper 56. FAO, Rome, Italy.

Amiri Deh ahmadi, S.R., Parsa, M., Nezami, A., and Ganjeali, A. 2011. The effects of drought stress at different phenological stages on growth indices of chickpea (Cicer arietinum L.) in greenhouse conditions. Iranian Journal of Pulses Research 1(2): 69-84. (In Persian with English Summary)

Amirmoradi, S., and Rezvani Moghadam. P. 2011. Effect of plant density and time of nitrogen application on morphological, phenological characteristics, yield and yield components of black Cumin (Nigella sativa L.). Journal of Horticultural Science 25(3): 251-260. (In Persian with English Summary)

Babaee, K., Amini Dehaghi, M., Modares Sanavi, S.A.M., and Jabbari, R. 2010. Water deficit effect on morphology, prolin content and thymol percentage of thyme (Thymus vulgaris L.). Iranian Journal of Medicinal and Aromatic Plants 26(2): 239-251. (In Persian with English Summary)

Bozin, B., Mimica-Dukic, N., Samojlik, I., Goran, A., and Igic, R. 2008. Phenolics as antioxidants in garlic (Allium sativum L., Alliaceae). Food Chemistry 111: 925-929.

Goldani, M., and Rezvani moghaddam, P. 2007. The effects of different irrigation regimes and planting dates on phenology and growth indices of three chickpea (Cicer arietinum L.) cultivars in Mashhad. Journal of Agricultural Science and Natural Resources 14: 229-242. (In Persian with English Summary)

Hamidvand, Y., Abdollahi, M.R., and Shaban, M. 2013. Investigation of growth analysis in chickpea (Cicer arietinum L.) cultivars under drought stress and Zn fertilizer. International Journal of Agriculture and Crop Sciences 5(5): 517-521.

Hamim, H., Banon, S., and Dorly, D. 2016. Comparison of physiological and anatomical changes of C3 (Oryza sativa L.) and C4 (Echinochloa crusgalli L.) leaves in response to drought stress. Earth and Environmental Science 31: 1-10.

Hanson, B.R., May, D., Voss, R., Cantwell, M., and Rice, R. 2003. Response of garlic to irrigation water. Agricultural Water Management Journal 58: 29-43.

Hayatu, M., Muhammad, S.Y., and Habibu, U.A. 2014. Effect Of water stress on the leaf relative water content and yield of some cowpea (Vigna unguiculata (L.) Walp.) genotype. International Journal of Scientific and Technology Research 3(7): 148-152.

Hejri, A. 2008. The effect of drought stress on growth indices, agronomical traits, yield and yield components of sunflower cultivars. MSc thesis. Agriculture Faculty. Isfahan Branch of Islamic Azad University, Isfahan, Iran. (In Persian with English Summary)

Hemmati, M.H., and Soleymani, A. 2014. A study about some physiological indices of sunflower growth under drought stress. International Journal of Advanced Biological and Biomedical Research 2(3): 553-563.

Hokmalipour, S., and Seyedsharifi, R. 2014. Effect of seed inoculation with plant growth promoting rhizobacteria (PGPR) affected by different levels of nitrogen and phosphorus fertilizers on the yield and some physiological parameters of barley (Hordeum vulgar L.). Iranian Journal of Field Crops Research 12(4): 822-833. (In Persian with English Summary)

Jamali, M.M. 2013. Investigate the effect of drought stress and different amount of chemical fertilizers on some physiological characteristics of coriander (Coriandrum sativum L.). International Journal of Farming and Allied Sciences 2(20): 872-879.

Johnson, M., Olaleye, O.N., and Kolawole, O.S. 2016. Antimicrobial and antioxidant properties of aqueous garlic (Allium sativum) extract against staphylococcus aureus and pseudomonas aeruginosa. British Microbiology Research Journal 14(1): 1-11.

Kafi, M., Borzoee, A., Salehi, M., Kamandi, A., Masoumi, A., and Nabati, J. 2010. Physiology of Environmental Stresses in Plant. Jihad Daneshgahi of Mashhad Press, Mashhad, Iran. 502 p. (In Persian)

Kafi, M., and Keshmiri, E., 2016. The changes in the physiological growth and the react of the salinity and number of irrigation water of two cumin cultivars (Cuminum cyminum L.). Jounal of Agroecology 8(3): 463-475. (In Persian with English Summary)

Karam, F., Masaad, R., Sfeir, T., Mounzer, O., and Rouphael, Y. 2005. Evapotranspiration and seed yield of field grown soybean under deficit irrigation conditions. Agricultural Water Management 75: 226-244.

Lafarge, T.A., and Hammer, G.L. 2002. Predicting plant leaf area production: shoot assimilate accumulation and partitioning, and leaf area ratio, are stable for a wide range of sorghum population densities. Field Crops Research 77: 137-151.

Lindquist, J.L., Arkebauer, T.J., Walters, D.T., Cassman, K.G., and Dobermann, A. 2005. Maize radiation use efficiency under optimal growth conditions. Agronomy Journal 97: 72-78.

Maleki Farahani, S., and Abdollahi, M. 2014. Effect of deficit irrigation on yield and yield components of two different species of balangu (Lallemantia royleana and iberica L.) from Mashhad and Urmia. Iranian Journal of Field Crops Research 12(3): 502-515. (In Persian with English Summary)

Moravčević, D.J., Bjelić, V., Moravčević, M., Gvozdanović Varga, J., Beatović, D., and Jelačić, S. 2011. Effect of plant density on the bulb quality and spring garlic yield (Allium sativum L.). Proceedings. In 46th Croatian and 6th International Symposium on Agriculture. Opatija, Croatia p. 554-557.

Pattangul, W., and Madore, M.A. 1999. Water deficit on raffinose family oligosaccharide metabolism in Coleus. Plant Physiology 121: 987-993.

Samvati, H.A. 2014. Effects of drought stress on some physiological indices in three varieties of onion (Allium cepa L.). First National Conference on Sustainable Agricultural Development with the Use of Crop Pattern. February 13, 2014, Hamedan, Iran. (In Persian)

Saravia, D., Farfan-vignolo, E.R., Gutierrez, R., De Mendiburu, F., Schafleitner, R., Bonierbale, M., and Khan, M.A. 2016. Yield and physiological response of potatoes indicate different strategies to cope with drought stress and nitrogen fertilization. The American Journal of Potato Research, 93: 288-295.

Sharif Rouhani, M., Kafi, M., Nezami, A., Jahedipour, F., Jahedipour, S., and Farajian, M.A. 2012. Irrigation regimes and depth of planting on yield of medicinal and industrial plant, shallots (Alliumm altissimum Regel.) in Mashhad weather conditions. In: National Conference on Natural Products and Medicinal Plants, Bojnourd, North Khorasan University of Medical Sciences, September 27- 26, 2012. (In Persian)

Tedeschi, A., Riccardi, M., and Menenti, M. 2011. Melon crops (Cucumis melo L., cv. Tendral) grown in a Mediterranean environment under saline–sodic conditions: Part II. Growth analysis. Agricultural Water Management 98: 1339-1348.