Effect of free and symbiotic nitrogen fixing bacterial co-inoculation on seed and seedling of soybean seeds produced under deficit water condition

Document Type : Scientific - Research

Authors

• Hamed Hadi ¹
• Jahanfar Daneshian ²
• Ahmad Asgharzadeh ³
• Aydin Hamidi ⁴
• Parisa Jonoubi ⁵
• Farshad Ghooshchi ¹
• Mohammad Nasri ¹

¹ Islamic Azad University, Varamin Branch, Varamin , Iran

² Seed and Plant Improvement Institue, Agricultural Research, Education, and Extension Organization, Karaj, Iran

³ Soil and Water Institute, Iran

⁴ Seed and Plant Certification and Registration Institute, Karaj, Iran

⁵ Department of Biology, Tarbiat Moalem University, Iran

10.22067/jag.v1i1.2654

Abstract

Effect of free and symbiotic nitrogen fixing bacteria on seed and seedling produced seeds under deficit irrigation was conducted in laboratory and field experiments in 2006. In laboratory of karaj’s Seed and Plant Research and Certificate Institute an experiment was conducted based on factorial in form of completely randomized design with four replications and in field’s of Islamic Azad University, Varamin Branch were split factorial in form of randomized completely block design with three replications. Treatments included water stress [Irrigation after 50 (Normal irrigation), 100 (Middle stress), 150 (Severe stress) mm evaporation from pan class A], Cultivar [Manokin & Williams and SRF×T3 Line] and inoculation [Inoculation with Bradyrhizobium japonicum, Bradyrhizobium japonicum co-inoculated with Azotobacter chroococcum, No seed inoculation]. Results showed that drought stress decreased the uniformity and germination speed and seedling emergence. Bacteria increased leaf dry weight, stem dry weight, leaf area and seedling vigor index but had no effect on emergence. In irrigation levels inoculated treatments had higher seedling length, leaf, stem, seedling dry weight and seedling vigor. Severs stress seeds inoculated with Bradyrhizobium japonicum had higher root dry weight than control. Therefore in seeds which were produced under deficit irrigation conditions, bacteria increased seedlings vigor.

Keywords

• Seedling vigor
• Seedling emergence
• Bradyrhizobium japonicum
• Azotobacter chroococcum

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References

Abdul-Baki, A.A., and Aderson, j.D., 1973. Vigor determination in soybean by multiple criteria. Crop Sci. 13,630-

633.

2- Antoun, H., Beauchamp, C.J., Goussard, N., Chabot, R. and Lalande, R. 1998. Potential of Rhizobium and

Bradyrhizobium species as plant growth promoting rhizobacteria on non-legumes: effect on radish (Raphanus

sativus L.). Plant Soil. 204,57-67.

3- Anonymus, 2008. Hand book for Seedling evaluation (3rd.ed). International SeedTesting Assosiation (ISTA),

Zurich, Switzerland.

4- Apte, R. and Shend, S.T., 1981. Studies on Azotobacter chroococcum. II. Effect of Azotobacter chroococcum on

germination of seeds of agricultural crops. Zentralblatt fur Bakteriologr-Parasiten Kunde. Infektion Skrankheien

und Hygiene. 136, 555-559.

5- Biswas, J.C., Ladha, J.K., Dazzo, F.B., Yanni, Y.G. and Rolfe B.G., 2000. Rhizobial inoculation influences seedling

vigor and yield of rice. Agron J. 92, 880-886.

6- Dakora. F.D., 2003. Defining new roles for plant and rhizobial molecules in sole and mixed plant cultures involving

symbiotic legumes. New Phytol. 157, 39-49.

7- Delouche, J.C., 1980. Environmental effects on seed development and seed quqlity. Hortscience 15 ,775-80.

8- Dorenbos, D.L., Mullen, R.E., and Shibles, R.M., 1989. Drought stress effects during seed fill on soybean seed

germination and vigour. Crop Sci. 29, 476-480.

9- Elis, R.H. and Roberts, E.H., 1980. Towards a rational basis for testing seed quality. In, Seed Production (ed. P.D.

Hebblethwaite), 605-645, Butterworths, London.

10- Hafeez, F.Y., Safdar, M.E., Chaudry, A.U. and Malik, K.A., 2004. Rhizobial inoculation improves seedling

emergence, nutrient uptake and growth of cotton. Aus. J. Exp. Agri. 44, 617-622.

11- Haji Boland, R., Ali Asghar Zadeh, N. And Mehrfar, Z.. 2004. Ecological study in two regions of Azarbyjan and its

effect on plant growth and mineral nutrition of wheat. J. Agricultural Science and Natural Resources, 8th year, No.

2, page 75-90.

12- Halmer, P., 2000. Commercial seed treatment technology. In: Seed Technology and its biological basis, Black, M.,

ed. Pp, 257-286.

13- Hampton, J.G., and Tekrony, D.M., 1995. Handbook of vigor test methods (3rd ed.) International Seed Testing

Association (ISTA) .Zurich, Switzerland.

تأثیر باکتری های تثبیت کننده آزادزی و همزیست … 63

14- Hunter, E.A., Glasbey ,C.A., and Naylor, R.E.L.,1984.The analysis of data from germination tests. J. Agr. Sci. 102,

207-231.

15- Kloepper, J.W., Scher, F.M., Labiret, E.M. and Tipping, B. 1986. Emergence promoting rhizobacteria, descriptions

and implications for agriculture., pp,155-164. in: Iron, sidrophores and plant disease. Ed., Swinburne , T.R.,

Plenume, New York.

16- Kloepper, J.W., Zablotowicz, R.M., Tipping, E.M. and Lifshitz, R., 1991. Plant growth promoting mediated by

bacterial rhizospherecolonizers., pp, 315-326. in: The rhizosphere and plant growth. Eds., Keister , D.L. and

Cregan, P.B., Kluwer Academic Publishers, Netherlands.

17- Marguire, J.D. 1962. Speed of germination-aid in selection and evaluation for seedling emergence and vigour. Crop

Sci. 2, 176-177.

18- McQuilken, M.P., Halmer, P. and Rhodes. D.J., 1998. Application of microorganisms to seeds. In: Formulation of

microbial biopesticdes: beneficial microorganisms, nematodes and seed treatment, Burges, H.D., ed. Pp, 255-285.

Kulwer Academic Publisher, The Netherlands.

19- Meghvansi, M.K., Kamal. P, and Mahna, S.K. 2005. Identification od pH tolerant Bradyrhizobium japonicum

strsins and their symbiotic effectiveness in soybean [Glycine max (L.) Merr] in low nutrient soil. Afr. j. Biotechnol.

4, 663-666.

20- Orchard, T., 1977. Estimating the parameters of plant seedling emergence. Seed Sci. Technol. 5, 61-69.

21- Ram, C., Kumari, P., Singh, O., and Sardana, R.K., 1989. Relationship between seed vigor tests and field

emergence in chickpea. Seed Sci. Technol. 17, 169-177.

22- Sobhani, A., Shirani Rad, A.H. N, Nakhoda, B., Fallahi Daghian A., 2000. Help determine LAI crops. Ministry of

Jihad-e-Agriculture - Agricultural extension and education research organization – Extension branch - part of

biotechnology research.

23- Vessey, J.K. 2003. Plant growth promoting rhizobacteria as biofertilizer. Plant Soil.255, 271-286.

24- Vieira, R.D., D.M. Tekrony, and D.B. Egli., 1991. Effect of drought stress on soybean seed germination and vigor.

J. seed technol. 16,12-21.

25- Zahir, A.Z., Arshad, M. and Frankenberger (Jr.), W.F., 2004. Plant growth promoting rhizobacteria: applications

and perspectives in agriculture. Adv agron. 81, 1-97.

26- Zhang. H, Daoust. F, Charles. TC, Driscoll. BT, Prithiviraj.B, Smith. DL., 2002. Bradyrhizobium japonicum

mutants allowing improved nodulation and nitrogen fixation of field grown soybean in short season area. J.Agri

Sci. 138, 293-300