The Effect of Mycorrhizal Fungus, Humic Acid and Vermicompost on Biochemical and Yield Properties of Silybum marianum

Document Type : Research Article

Authors

• Foroozan Nikkhah Naeeni ¹
• alireza ladan moghdam ²
• Pezhman Moradi ³
• Vahid Abdoosi ¹
• Mehdi Rezaei ⁴

¹ Department of Horticultural Science, Science and Research Branch, Islamic Azad University, Tehran, Iran

² Department of Horticultural Science, Garmsar Branch, Islamic Azad University, Garmsar, Iran

³ Department of Horticultural Science, Saveh Branch, Islamic Azad University, Saveh, Iran

⁴ Department of Horticultural Science, Faculty of Agriculture, Shahrood University of technology, Iran

10.22067/agry.2021.68348.1012

Abstract

Introduction
milk thistle (Silybum marianum) is one of the most popular medicinal plants of most developed countries. In the past, milk thistle leaves has been used to treat biliary and gastrointestinal diseases. It has valuable effects on the prevention and treatment of long-term complications of diabetes, renal failure and nerve cell disorders. Silibinin is the most important flavonoid in the plant that plays an important role in prostate cancer treatment. Humic Materials are part of the organic matter of humus soils resulting from the physical, chemical and microbiological deformation or homophication of biomolecules in the soil. This study aims at investigating the effect of organic and biological fertilizers including humic acid, vermicompost and two species of mycorrhizal fungi on physiological and morphological traits of Silybum marianum.
Materials and Methods
This study was conducted in the spring in Gorgan. This study was conducted as factorial experiments using Completely Randomized Design (CRD). Treatments included four levels of vermicompost (0, 25, 50, and 75%), four levels of humic acid fertilizer (0, 25, 50 and 75 mg / L) and two species of mycorrhizal fungi (Glomus mosseae and Glomus intraradices). Yield, 1000 - grain weight, leaf area, capitule number, chlorophyll a, chlorophyll b, total chlorophyll, carotenoids, soluble sugars and protein were measured in the plant. All measurements were carried out in three replications, and mean comparisons were made through the Duncan test (P<0.05) using SPSS-22 software package.
Results and Discussion
The results of data analysis of variance showed that all the studied properties are affected by the interaction of the applied treatments. The highest Silybum marianum yield was obtained in Glomus mosseae third level vermicompost and first humic acid level equal to 2296 kg / ha. Also, the lowest grain yield was obtained in the control treatment (435 kg / ha). The reason for the increase in yield can be attributed to the supply of nutrients, water supply and improving the respiratory status of the plant due to the use of organic and biological fertilizers. Also, the presence of mycorrhiza fungi and vermicompost has improved the physical condition of the soil and increased the ability of the plant to use soil water. The highest leaf area (3.96 cm²) was observed in the fourth level of humic acid (75 mg / L) and vermicompost (75%) in both types of Glomus mosseae and Glomus intraradices. The highest amount of total chlorophyll in the plant (15.26 mg / g fresh weight) was related to humic acid 50 g / l + mycorrhizal fungus Glomus mosseae at 25% vermicompost level. Mycorrhizal fungus improves nitrogen uptake, which plays a key role in plant chlorophyll structure. On the other hand, nitrogen is the most important element in protein synthesis, which is in favorable condition with the increase of mycorrhizal fungus and leads to an increase in the amount of protein and soluble sugar. The results have shown that use of humic acid has positive effects on the studied plant characteristics. The highest protein (3.65 mg / wet weight) was obtained in the third level of humic acid and Glomus intraradices and the lowest protein (0.92 mg /wet weight) in control treatment.
Conclusion
The use of organic compounds as fertilizer improves the physical, chemical and biological properties of soil. Also increases plant growth by providing the plant with the necessary elements. In general, the results showed that the use of organic and bio-fertilizers can improve the status of Silybum marianum and these compounds can reduce the use of chemical fertilizers to prevent the adverse effects of these fertilizers on human health and the environment.
 

 
 
 
 

Keywords

• Bio-fertilizers
• Capitule
• Carotenoids
• Milk thistle
• Mycorrhizal

20.1001.1.20087713.1401.14.3.9.9

Main Subjects

• Agroecology and Indigenous Knowledge

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References

Abdollahzare, S., Fateh, E., and Aeineband, A., 2011. Investigation into different sowing dates and fertilization methods (chemical and organic) on yield and yield components of milk thistle (Silybum marianum L.). Journal of Plant Productions 35(1): 129-143. (In Persian with English Summary)

Afek, U., Menga, G.A., and Johnsen, E.L.V., 1990. Filecr of pythiutn ultimum and metalaxyl treatments on root length and mycorrhizal colonization of cotton, Onion and pepper. Plant Disease 74: 117-120. (In Persian with English Summary)

Aiken, G.R., Diane M.M., Wershaw, R., and MacCarthy, P., 1984. An Introduction to Humic Substances in Soil, Sediments, and Water. A Wiley-Interscience Publication. Chapter One. 1-9.

Akbarinia, A., Ghalavand, A., Sefidcon, F., Rezaee, M.B., and Sharifi, A., 2003. Study on the effect of different rates of chemical fertilizer, manure, and mixture of them on seed yield and main, compositions of essential oil of ajowan (Trachyspermum copticum). Pajouhesh and Sazandegi 61: 32-41. (In Persian with English Summary)

Alikaridis, F., Papadakis, D., Pantelia, K., and Kephalas, T., 2000. Flavonolignan production from Silybum marianum transformed and untransformed root Cultures. Fitoterapia 71:379-384. DOI: 10.1016/s0367-326x(00)00134-9.

Amiri, M.B., Rezvani Moghaddam, P., and Jahan, M., 2017. Effects of organic acids, mycorrhiza and rhizobacteria on yield and some phytochemical characteristics in low-input cropping system. Journal of Agricultural Science and Sustainable Production 27(1): 45-61. (In Persian with English Summary)

Aronon, D.I., 1949. Copper enzymes in isolated chloroplasts. Polyphennoloxidase in Beta vulgaris. Plant Physiology 24(1): 1-150. DOI: org/10.1104/pp.24.1.1

Aslani, Z., Hassani, A., Rasooli Sadaghiyani, M., Sefidkon, F., and Barin, M., 2011. Effect of two fungi species of arbuscular mycorrhizal (Glomus mosseae and Glomus intraradices) on growth, chlorophyll contents and P concentration in Basil (Ocimum basilicum L.) under drought stress conditions. Iranian Journal of Medicinal and Aromatic Plants 27(3): 471-486. (In Persian with English Summary). DOI: 10.22092/IJMAPR.2011.6388.

Atiyeh, R., Lee, S., Edwards, C., Arancon, Q., and Metzger, J., 2002. The influence of humic acids derived from earthworm processed organic wastes on plant growth. Bioresour. Technology 84(1): 7–14. DOI: org/10.1016/S0960-8524(02)00017-2

Auge, R.M., and Duan, X., 1991. Mycorrhizal fungi and non hydraulic root signals of soil drying plant physiology. Environmental and Stress Physiology 97: 821-82. DOI: 10.1104/pp.97.2.821

Azizi, M., Lakzian, A., and Baghani, M., 2004. Investigation of the effect of different amounts of vermicompost on growth indices and amount of modified basil essential oil. Proceedings of the Second Conference on Medicinal Plants, Shahed University, Iran. February, 8-27: 62. (In Persian with English Summary)

Barzaghi, N., Crema, F., Gatti, G., Pifferi, G., and Perucca, E., 1990. Pharmacokinetic studies on IdB 1016, a silybin-phosphatidyl choline complex, in healthy human subjects. European Journal of Drug Metabolism and Pharmacokinetics 15: 333-8. DOI: 10.1007/BF03190223

Barzegar Alvirdi, M., Afshari, H., Borhan, N., and Lai Ghanbar Zadeh, B., 2013. Effect of planting date and symbiosis of mycorrhizal fungi on physiological characteristics and effective substances of three Iranian fennel (Foenicumlum vulgare Mill) cultivars. Eco-Phytochemical Journal of Medicinal Plants 1(2): 64-75. (In Persian with English Summary)

Benda, L., Dittrich. H., Ferenzi, P., Frank, H., and Wewalk, F., 1980. The influence of therapy with Silybum on the survival rate of patients with liver cirrhosis. Wien Klin Wochenschr 92: 678-83.

Blumenthal, E., and Busse, W., 1998. Monographs: Therapeutic guide to herbal medicines. Austin: American Botanical Council.

Calvet, C., Pinochet, J., Hernandez–Dorrego, A., Estaun, V., and Camprubi, A., 2001. Field micro plot performance of the Peach almond hybrid GF – 677 after inoculation with arbuscular mycorrhizal fungi in a replant soil infested with root K not nematodes. Mycorrhizal 10: 295–300. DOI: org/10.1007/PL00009998

Das, A., Kamal. S., Akhtar Shakil, N., Sheramcti, I., Oelmuller, R., Dua, M. Tureja, N., Kumar, A., and Varma, A., 2012. The root endophyte fungus Piriformospora indica leads to early flowering, higher biomass and altered secondary metabolites of the medicinal plant. Coleus forskohlii, Plant Signaling and Behavior 7(1):103– 112. DOI: 10.4161/psb.7.1.18472

Dell Agnola, G., and Nardi, S., 1987. Hormone like effect of enhanced nitrate uptake induced by depolycondensed humic fractions obtained from Allolobopbora rosea and A. caliginosa facces. Biology and Fertility of Soils 4: 115-118.

Dursun, A., Guvenc, I., and Turan, M., 2002. Effects of different levels of humic acid on seedling growth and macro and micronutrient contents of tomato and eggplant. Acta Agrobotanica 56: 81-88. DOI: 10.5586/aa.2002.046

El-Bassiouny, H.S.M., Bakry, A.B., Abd El-Monem Attia, A., and Abd Allah, M.M., 2014. Physiological role of humic acid and nicotinamide on improving plant growth, yield, and mineral nutrient of wheat (Triticum durum) grown under newly reclaimed sandy soil. Agricultural Sciences 5: 687-700. DOI: 10.4236/as.2014.58072.

Eman, A.A., El-Monem, A.B.D., Saleh, M.S., and Mostafa, E.A., 2008. Minimizing the quantity of mineral nitrogen fertilizers on grapevine by using humic acid, organic and biofertilizers. Research Journal of Agriculture and Biological Sciences 4(1): 46-50.

EneJi, A.E., An, R., Islam, P., and Amalu, U.C., 2013. Nitrate retention and physiological adjustment of maize to soil amendment with superabsorbent polymers. Cleaner Production 18: 1-7. DOI: org/10.1016/j.jclepro.2013.02.027

Fatma, E.M., El-zamik, I., Tomader, T., ElHadidy, H.I., Abd El-Fattah, L., and Seham Salem, H., 2006. Efficiency of biofertilizers organic and in organic amendments on growth and essential oil of marjoram (Majorana Hortensis) plant growth in sandy and calcareous. Agriculture Microbiology Department, Faculty of Agriculture, Zayazig University and Soil Fertility and Microbiology Department, Desert Research Center Cairo Egypt.

Fayyaz, M., Zare, S., and Ashori, P., 2011. Identification and distribution of pharmaceutical and industrial crops of Chaharmahal-va-Bakhtiari province, Institute of Forests and Rangelands Research. (In Persian with English Summary)

Fernandez, R.I., Benloch, M., Bartanco, D., Duenas, A., and Ganan, J.A.G., 1996. Response of olive trees to foliar application of humic substance extracted from leonardite. Scientia Horticulturae 66(9): 200. DOI: org/10.1016/S0304-4238(96)00914-4

Fintelmannz, M., 1999. Phytotherapy and its uses in gastrointestinal condititions. Medicinal Plants 57: 48-52.

Gholami, A., Akbari, I., and Abbas Dokht, H., 2015. Study the effects of bio and organic fertilizers on growth characteristics and yield of fennel (Foeniculum vulgar). Journal of Agroecology 7(2): 215-224. (In Persian with English Summary)

Graham, J.H., and Egel, D.S., 1988. Phytophthora root development on mycorrhizal and phosphorus fertilized nonmycorrhizal sweet orange seedlings. Plant Disease 72: 614. DOI: 10.1094/PD-72-0611.

Greenlee, H., Abascal, K., Yarnell, E., and Ladas, E., 2007. Clinical Applications of Silybum marianum in Oncology. Integrative Cancer Therapies 6(2): 158-165. DOI: 10.1177/1534735407301727

Hajghani, M., Ghalavand, A., and Modarres Sanavy, S.A.M., 2017. Evaluation of yield, yield components and growth indices of safflower (Carthamus tinctorius L.) in conventional and organic farming systems. Journal of Agroecology 9(1): 15-30. (In Persian with English Summary)

Han, H.S., and Lee, K.D., 2005. Plant growth promoting rhizobacteria effect on antioxidant status, photosynthesis, mineral uptake and growth of lettuce under soil salinity. Agricultural and Biological Sciences 6: 155- 180.

Harris, D., and Paul, E.A., 1987. Carbon Requirements of Vesicular– Arbuscular, Mycorrhizae, 93–103. In: Safir, G.E. (Ed.). Ecophysiology of VA Mycorrhiza Plants. CRC press, Boca Raton, USA, 224 p.

Kanel, S., and Choi, H., 2007. Adsorption of humic acid onto nanoscale zerovalent iron and its effect on arsenic removal. Environment Science Technology 41(6): 2022–2027. DOI: org/10.1021/es0616534

Khalesro, S., and Malekian, M., 2017. Effects of vermicompost and humic acid on morphological traits, yield, essential oil content and component in organic farming of ajwan (Trachyspermum ammi L.). Iranian Journal of Medicinal and Aromatic Plants 32(6): 968-980. (In Persian with English Summary)

Luper, S., 1998. A review of plant used in the treatment of liver disease: Part 1. Alternative Medicine Review 3: 410-421.

Mackowiak, C.L., Grossl, P.R., and Bugbee, B.G., 2001. Beneficial effects of humic acid on micronutrient availability on Wheat. Soil Science 65: 1744–1750. DOI: .org/10.2136/sssaj2001.1744

Stefan, M., Ailiesei, O., Ungureanu, E., Dunca, S., Nimitan, E., & Artenie, V., 2005. Study of a microbial inoculation on several biochemical indices in sunflower (Helianthus anuus L.). Genetics and Molecular Biology 11-14.

Mikkelsen, R.I., 2005. Humic materials for agriculture. Better Crops 89(3): 6-8.

Mohamedi, R.S.R., and Ahmed. M.A., 2009. Effect of biofertilizers and humic acid on control of dry root rot disease and improvement yield quality of mandarin (Citrus reticulate Blanco). Journal of Agricuture and Biological Sciences 5(2): 127–137. (In Persian with English Summary)

Nargesi Alipour, N., Akhzari, D., and Fatahi, B., 2016. Investigation of the interaction of different levels of zeolite and compost on some soil properties and soil performance. Conservation of Plant Ecosystems 4(9): 151-164. (In Persian with English Summary)

O’Donnell, R.W., 1973. The auxin-like effects of humic preparation from leonardite. Soil Science 116: 106–112. DOI: org/10.1097/00010694-197308000-00007

Omid Beigi, R., 1997. Ways of Production and Processing of Medicinal Plants. Volume II. Publishers Designers Publishing. (In Persian)

Omid Beigi, R., 1998. Investigation of silymarin production and intermittent silage in wild plant cultivation with wild and agronomic seeds. Iranian Journal of Agricultural Sciences 29(2): 413. (In Persian with English Summary)

Pilanali, N., and Kaplan, M., 2003. Investigation on the effect on nutrient uptake of humic acid application of different forms to strawberry plant. Journal of Plant Nutrition 26(4): 835-843. DOI: org/10.1081/PLN-120018568

Rabie, G.H., 2005. Influence of arbuscular mycorrhizal fungi and kinetin on the response of mungbean plants to irrigation with seawater. Mycorrhiza 15: 225–230. DOI: 10.1007/s00572-004-0345-y.

Ram, G., Bhan, K.M., Gupta, K.K., Thaker Brijesh, U., and Pal, S., 2005. Variability pattern and correlation studies in Silybum marianum Gaertn. Fitoterapia 76: 147-143. DOI: 10.1016/j.fitote.2004.10.006

Salwa, A., 2011. Effect of amendments humic and amino acids on increases soils fertility yields and seeds quality of peanut and sesame on sandy soils. Agriculture and Biological Science 7(1): 115-125.

Saydi, Z., Fateh, E., and Aynehband, A., 2017. Effect of different sources of nitrogen and organic fertilizers on yield and yield components of ajowan (Trachyspermum ammi L.). Journal of Agroecology 9(1): 115-128. (In Persian with English Summary)

Schandalik, R., Gatti, G., and Perucca, E.P., 1992. Pharmacokinetics of silybin in bile following administration of silipide and silymarin in cholecystectomy patients. Arzneimittelforschung 42: 964-8.

Selim, E.M., Mosa, A.A., and El Ghamry, A.M., 2009. Evaluation of humic substances fertigation through surface and subsurface drip irrigation systems on potato grown under Egyptian sandy soil conditions. Agricultural Water Management 96: 1218–1222. DOI:org/10.1016/j.agwat.2009.03.018

Sherameti, I., Tripathi, S., Varma, A., and Oclmuller, R., 2008. The root colonizing endophyte Pirifomospora indica confers drought tolerance in Arabidopsis by stimulating the expression of drought stress related genes in leaves. Mol Plant MicrobInteract 21: 799–807. (In Persian with English Summary)

Smith, S.E., and Read, G.J., 1997. Mycorrhizal Symbiosis. Academic press, San Diego California, pp.126–60.

Stevenson, F.J., 1994. Humus Chemistry. Genesis, Composition, Reactions. 2^nd Edition, John Wiley and Sons, New York. 1-96.

Varma, P.N., and Talwar, S.K., 1980. Chemical Investigations of Silybum marianum. Planta Med 38: 377.

Wang, H.C., and Kao, K.N., 1988. G-banding in plant chromosomes. Genome 30: 48-51.

Yemm, E., and Willis, A.J., 1954. The estimation of carbohydrate in plant extracts by Anthrone. Biochemical Journal 57: 508-514. DOI: 10.1042/bj0570508

Zargari, A., 1996. Medicinal Plants. Fifth Edition. Institute of Publishing and Printing, Tehran University Publishing, Tehran 136. (In Persian)