Evaluation the Effect of Inoculation Time of Plant Growth Promoting Rhizobacteira on Macroelements Uptake and Yield of Tomato (Solanum lycopersicum L.(

Document Type : Scientific - Research

Authors

• peyman sheikhalipour ¹
• Saheb Ali Bolandndnazar ²
• Mohammad Reza Sarikhani ²

¹ University of Mohaghegh Ardabili

² University of Tabriz

10.22067/jag.v11i2.64681

Abstract

Introduction
Poor soil fertility is one of the main problems that limit the successful agricultural production and the global economy. Therefore, it is necessary to regulate or correct poor soil fertility, by providing vital nutrients for optimum plant growth. Improving soil fertility through fertilizer can increase crop production per unit area. But excess use of chemical fertilizers, in addition to disrupting the biological balance, nutrition and physical properties of soils, causes to environmental hazards, pollution of surface and ground water resources. Biofertilizers can reinforcement soil fertility  and provide the required plant nutrition with respect of sustainable agriculture. These organisms not only stimulate the growth of plants by helping the specific elements, but also reduce disease, improve soil structure and consequently increase the quantity and quality of their product. Pseudomonas, Bacillus, Azospirillum, Azotobater and Rhizobium are the most important plant growth promoting rhizobacteria (PGPR). Several mechanisms have been suggested by which PGPR can promote plant growth, including auxins, enhancing stress resistance, asymbiotic N2 fixation, solubilization of inorganic phosphate, mineralization of organic phosphate or other nutrients and inhibiting the growth of pathogenic microorganisms.
Materials and methods
In order to determine the best time of application of Pseudomonas ssp. strains (sp.S10-1 + sp.S19-1 , sp.S14-3 + sp.S19-1 , sp.S19-1+ sp.S21-1) as a plant growth promoting rhizobacteria on growth and nutritional characteristics of tomato (Lycopersicon esculentum Mill cv. Super Chief) an experiment based on randomized complete block designed with 8 treatments and 3 replications in field of research station Khalatpoushan Faculty of Agriculture, Tabriz University. Treatments used in this experiment included : 1-  Inoculation with Pseudomonas bacteria sp.S10-1 + Pseudomonas sp.S19-1 during seed planting in the nursery, 2- Inoculation with Pseudomonas bacteria sp.S14-3+Pseudomonas sp.S19-1  during seed planting  in the nursery, 3- Inoculation with Pseudomonas bacteria sp.S19-1+Pseudomonas sp.S21-1  during seed planting  in the nursery, 4- Inoculation with Pseudomonas bacteria sp.S10-1 + Pseudomonas sp.S19-1 during planting seedlings in the main land 5- Inoculation with Pseudomonas bacteria sp.S14-3+Pseudomonas sp.S19-1 during planting seedlings in the main land 6- Inoculation with Pseudomonas bacteria sp.S19-1+Pseudomonas sp.S21-1 during planting seedlings in the main land 7- control treatment without bacteria inoculation 8- fertilizer treatment (according to the soil test).  
Results and discussion
The result showed that the effects of the timing of bacteria inoculation on tomato characters such as yield, fruit size and the percentage of marketable fruit, percentage of K, vitamin C is meaningful and the highest amount of yield, fruit size, the percentage of marketable fruit, percentage of K in fruit was in T2 treatment concluded (4130.9 g m-2 and 60.22 and 31.18 and 4.7 mg g-1) respectively and the highest amounts of the vitamin C in T3 treatment as 7.02 mg 100g-1 fruit weight. However the other measured traits, including percentage of percentage of N, P in plant leaves, length and diameter of fruit and percentage of the dry matter did not affected by the timing of bacteria inoculation. According to the result  timing of bacteria inoculation has a significant effect on  yield and quality improvement of tomato.
Conclusion
 The results of this research showed that tomato seed inoculation with the Pseudomonas ssp. strains including (sp.S10-1 + sp.S19-1 , sp.S14-3 + sp.S19-1 , sp.S19-1+ sp.S21-1)  compared with soil inoculation  of  Pseudomonas ssp. strains significantly increased yield, vitamin C, potassium content of fruit and percentage of marketable fruit. However, the increase in the amount of potassium, phosphorous and nitrogen concentration in plant leaves was not significant. In addition to increasing the yield and quality of tomato, tomato seed inoculation with the Pseudomonas ssp. strains compared with soil inoculation with the strains Pseudomonas is considered a better method.

Keywords

• Biological Composts
• Pseudomonas
• Potassium
• Phosphor
• Nitrogen
• yield of the tomato

20.1001.1.20087713.1398.11.2.2.9

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References

Adams, P., and Holder, R. 1992. Effects of humidity, Ca and salinity on the accumulation of dry matter and Ca by the leaves and fruit of tomato (Lycopersicon esculentum). Agricultural Science 5: 37-44.

Anac, D., and Colocoglu, H. 1995. Response of Some Major Crops to K Fertilization. In: K. Mengel and A. Krauss (Eds.). K Availability of Soils in West Asia and North Africa - Status and Perspectives. International Potash Institute 235-247.

Antoun, H. 2002. Field and green house trials performed with phosphate solubilizing bacteria and fungi.pp. Proceedings of the 15th International meeting on Microbial Phosphate Solubilization. Salamanca University, 16-19 July 2002. Salamanca, Spain 235-237.

Antoun, H., and Kloepper, J.W. 2001. Plant growth promoting rhizobacteria. Encyclopedia of genetics. Eds. S Brenner and J Miller 1477-1480.

A.O.A.C. 1980. Official Methods of Analysis, 13th ed. Association of Official Analytical. Chemists. Washington D.C 376-384.

Ashoor, S.H., Monte, W.C., and Welty. J. 1984. Liquid Chromatographic Determination of Ascorbic Acid in Foods. Journal of the Association of Official Analytical Chemists 67(1):78-80.

Aslantaş, R., Çakmakçi, R., and Sahin, F. 2007. Effect of plant growth promoting rhizobacteria on young apple tree growth and fruit yield under orchard conditions. Scientia Horticulturae 111: 371-377.

Aseretal, G.K. 2008. Biofertilizers improve plant growth, fruit yield, nutrition, metabolism and rhizosphere enzyme activities of Pomegranate (Punica granatum L.). Bioresource Technology 97(6): 98-109.

Badr, MA. 2006. Efficiency of K-feldspar combined with organic materials and silicate dissolving bacteria on tomato yield. Journal of Applied Sciences Research 2: 1191-1198.

Balliu, A., and Ibro, V. 2002. Influence of different levels of potassium fertilizers on growth, yield and ascorbic acid content of tomato fruit grown in non-heated greenhouse. Acta Horticulturae 579:385-388.

Besford, R.T., and Maw, G.A. 1975. Effects of potassium nutriation of tomato plant growth and fruit development. Plant and Soil 42: 395- 412.

Brown, M.E. 1974. Seed and root bacterization. Annual Review of Phytopatliol 12: 181-197.

Bryson, G.M., and Barker, A.V. 2002. Determination of optimal fertilizer concentration range for tomatoes. Communications in Soil Science and Plant Analysis 33: 759-777.

Cakmakci, R., Donmez, F., Aydın, A., and Sahin, F. 2006. Growth promoting of plants by plant growth-promoting rhizobacteria under greenhouse and two different field soil conditions. Soil Biology and Biochemistry 38: 1482-1487.

Chakraborty, U., Chakraborty, B., and Basnet, M. 2006. Plant growth promotion and induction of resistance in Camellia sinensis by Bacillus megaterium. Journal of Plant Physiology 127: 1617-1625.

Chapagain, B.P., and Wiesman, Z. 2004. Effect of potassium magnesium chloride in the fertigation solution as partial source of potassium on growth, yield and quality of greenhouse tomato. Scientia Horticulturae 99: 279-288.

Copetta, A., Lingua, G., and Berta, G. 2006. Effects of three AM fungi on growth, distribution of glandular hairs, and essential oil production in Ocimum basilicum L. var. Genovese. Mycorrhiza 16: 485-494.

Dagigian, N., Habibi, D., Madani, H., and Sajedi, N.E. 2011. To evaluate the effect the best method and timing of PGPR on the absorption of nitrogen, phosphorus, potassium and yield of beans (Phaseolus vulgaris L.). Journal of Crop Ecophysiology 3: 94-100. (In Persian with English Summary)

Dorais, M., Papadopulos, A.P., and Gosselin, A. 2001. Influence of electrical conductivity management on greenhouse tomato yield and fruit quality. Agronomie 21: 367-383.

Economakis, C., and Daskalaki, A. 2003. Effect of potassium nutrition on yield and quality of tomato plants grown with nutrient film technique under sodium chloride saline conditions. Acta Horticulturae 609: 337-339.

Egamberberdiyeva, D., and Hoflich, G. 2003. Influence of growth- promoting bacteria on the growth of wheat in different soils and temperatures. Soil Biology and Biochemistry 35: 973-978.

Egamberdiyeva, D. 2005. Plant-growth promoting rhizobacteria isolated from a Calcisol in a semiarid region of Uzbekistan: biochemical characterization and effectiveness. Journal of Plant Nutrition and Soil Science 168: 94-99.

FAO (Food and Agricultural Organization). FAO State, core production. 2011. Available online at: http://faostat. fao. org/site/291/default. aspx.

Flores, FB., Sanchez-Bel, P., Estan, M.T., Martinez-Rodriguez, M.M., Moyano, E., Morales, B., Compos, J.F., Garcia-Abellan, J.O., EgeaI Fernandez-Garcia, N., Romojaro, F., and Bolarin, MC.C. 2010. The effectiveness of grafting to improve tomato fruit quality. Scientia Horticulturae 125:211-217.

Fawzy, Z.F., Behairy, A.G., and Shehata, S.A. 2005. Effect of potassium fertilizer on growth and yield of sweet pepper plants (Capsicum annuum, L.). Egyptian Journal of Agricultural Research 2(2): 599-610.

Fawzy, Z.F., El-Nemr, M.A., and Saleh, S.A. 2007. Influence of levels and methods of potassium fertilizer application on growth and yield of eggplant. Journal of applied sciences research 3(1): 42-49.

Glick, B.R. 1995. The enhancement of plant growth by free-living bacteria. Canadian Journal of Microbiology 41: 109-117.

Gupta, C.R., and Sengar, S.S. 2000. Response of tomato (Lycopersicon esculentum Mill.) to nitrogen and potassium fertilization in acidic soil of Bastar. Journal of Vegetation Science 27(1): 94-95.

Han, H.S., and Lee Supanjani, K.D. 2006. Effect of co-inoculation with phosphate and potassium solubilizing bacteria on mineral uptake and growth of pepper and cucumber. Plant Soil and Environment 52(3): 130–136.

Han, H.S., and Lee, K.D. 2005. Phosphate and potassium solubilizing bacteria effect on mineral uptake, soil availability and growth of eggplant. Research Journal of Agriculture and Biological Sciences 1(2): 176-180.

Hanson, J.B. 1967. Roots selectors of plants nutrients. Plant food Rev. Spring P: 45-53.

Heinrichs, D.E., Rahn, A., Dale, S.E., and Sebulsky, M.T. 2004. Iron transport systems in pathogenic bacteria: Staphylococcus, Streptococcus, and Bacillus. Pp. 387-401. In: crosa JH, Mey AR and Payne SM, (eds.) Iron Transport in Bacteria. American Society of Agronomy. Wisconsin, DC. Hort 731: 115-120.

Illmer, P., and Schinner, F. 1995. Solubilization of inorganic calcium phosphates.Soil Biol. Biochem 46: 257-263.

Jahan, M., Nasiri Mahalati, M., Salari, M. D. and Gorbani, V. R. 2010. The effects of time use of manure and use of bio-fertilizers on the quantity and quality types of summer squash (Cucurbita pepo L.). Iranian Journal of Field Crops Research 8(4):726-737. (In Persian with English Summary).

Jeon, J.S., Lee, S. S., Kim, H. Y., Ahn, T. S. and Song, H. G. 2003. Plant growth promotion in soil by some inoculated microorganisms. Journal of Microbiology 41: 271-276

Kanai, S., Ohkura, K., Adu-Gyamfi, J.J., Mohapatra, P.K., Nguyen, N.T., Saneoka, H., and Fujita, K. 2007. Depression of sink activity precedes the inhibition of biomass production in tomato plants subjected to potassium deficiency stress. Journal of Experimental Botany 58: 2917–2928.

Khorramdel, S., Koocheki, H.R., Nassiri Mahallati, M., and Ghorbani, R. 2010. Effects of biological fertilizers on yield and components yield Nigella sativa medicinal plants. Iranian Journal of Field Crops Research 8(5): 758-766. (In Persian with English Summary)

Kloepper, J.W., Reddy, M.S., Rodriguez-Kabana, R., Kenney, D.S., Kokalis-Burelle, N., Martinez-Ochoa, N., and Vavrina, C.S. 2004. Application for rhizobacteria in transplant production and yield enhancement. Acta Horticulturae 631: 217–229.

Lester, G.E., and Jifon, J.L. 2007. Foliar applied potassium: Effects on cantaloupe quality. Acta Horticulturae 731: 115-120.

Lester, G.E., Jifon, J.L., and Makus, D.J. 2006. Supplemental foliar potassium applications with or without a surfactant can enhance netted muskmelon quality. Horticultural Science 41(3): 741-744.

Lester, G.E., Jifon, J.L., and Rogers, G. 2005. Supplemental foliar potassium applications during muskmelon fruit development can improve fruit quality, ascorbic acid, and beta-carotene contents. Journal of the American Society for Horticultural Science 130: 649–653.

Majumdar, S.P., Meena, R.L., and Baghel, G.D.S. 2000. Effect of levels of compaction and potassium on yield and quality of tomato and chilli crops grown on highly permeable soils. Journal of the Indian Society of Soil Science 48(2): 215-220.

Mikhailouskaya, N., Kurilovitch, N., and Djusova, S. 2003. The effect of phosphorus and potassium fertilization on the efficiency of flax bacterization. Pp. 43-47. Proceedings of the Latvia University of Agriculture. Jelgava. Latvia.

Nanadal, J.K., Ramesh, V., and Pandey, U.C. 1998. Effect of phosphorus and potassium on growth yield and quality of tomato. Journal of Potassium Research 14(1):44-49.

Okaz, A.M.A., El-Ghareib, E.A., Kadry, W., Negm, A.Y., and Zahran, F.A.F. 1994. Response of lentil plants to potassium and phosphorusapplication in newly reclaimed sandy soils. Proc. 6th Conf. Agron., Al-Azhar Univ., Cairo, Egypt, Sept. II: 753-771.

Olivera, M., Iribarne, C., and Lluch, C. 2002. Effect of phosphorus on nodulation and N2 fixation by bean (Phaseolus vulgaris). Proceedings of the 15th International Meeting on Microbial Phosphate Solubilization. Salamanca University, 16-19 July, Salamanca, Spain.

Olsen, S.R., and Sommers, L.E. 1982. Phosphorus. P. 403-430, In: Page, A.L. (Eds.), Methods of Soil Analysis. Part 2. 2nd ed. Argon. Mongr. 9. ASA and SSSA, Madison, WI.

Padem, H., and Ocal, A. 1999. Effects of humic acid applications on yield and some characteristics of processing tomato. Acta Horticulturae 487: 159-164.

Patidar, M. 2001. Integrated nutrient management in sorghum (Sorghum bicolor) and its resideul effect on wheat (Triticum aestivum) Indian Jornal of agricultural Sciences 71 (9): 587 – 590.

Rodrigues, V.A., Mazza, S.M., Martinez, G.C., and Ferrero, A.R. 2005. Zn and K influence in fruit sizes of ‘Valencia’ orange. Revista Brasileira de Fruticultura 27(1): 132-135.

Rodriguez, H., and Frage, R. 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology Advances 17: 319-339.

Rokhzadi, A., Asgharzadeh, A., Darvish, F., Nour- Mohammadi, G. and Majidi, E. 2008. Influence of plant growth-promoting rhizobacteria on dry matter accumulation and yield of chickpea (Cicer arietinum L.) under field conditions. American-Eurasian Journal of Agricultural and Environmental Science 3 (2): 253-257.

Sahin, F., Kotan, R., Demirci, E., and Miller, S.A. 2000. Domates ve biber bakteriyel leke hastaligi ile biyolojik savasta actigard ve bazi antagonistlerin etkinligi. Ataturk Universitesi Ziraat Fakultesi Dergisi 31: 11-16.

Salantur, A., Ozturk, A., and Akten, S. 2006. Growth and yield response of spring wheat (Triticum aestivum L.) to inoculation with rhizobacteria. Journal of Soil Environment 52(3): 111-118.

Salantur, A., Ozturk, A., Akten, S., Sahin, F., and Donmez, F. 2005. Effect of inoculation with nonindigenous and indigenous rhizobacteria of Erzurum (Turkey) origin on growth and yield of sipring barley. Plant and Soil 275: 147-156.

Saleh Rastin, N. 1998. Biological fertilizers. Journal of Soil and Water Sciences 12: 1-36. (In Persian with English Summary)

Saleh Rastin, N. 2001. Biological fertilizers and their role in sustainable agriculture. Collection of Researches on the Necessi ty of Industria Production of Bio- fertilizers 1-54.

Sampaio, R.A. and Fontes, P.C.R. 2000. Composição quimica e qualidade de frutos do tomateiro em função da adubação potassica. Ciência Agricola, Rio Largo 5(1):65-73.

Shaalan, M.N. 2005. Influence of biofertilizers and chicken manure on growth, yield and seeds quality of (Nigella sativa L.) plants. Egyptian Journal of Agricultural Research 83: 811-828.

Sheng, X.F., Zhao, F., He, L.Y., Qiu, G., and Chen, L. 2008. Isolation and characterization of silicate mineral solubilizing Bacillus globisporus Q12 from the surfacees of weathered feldspar. Canadian Journal of Microbiology 54: 1064-1068.

Sugumaran, P., and Janarthanam, B. 2007. Solubilization of potassium containing minerals by bacteria and their effect on plant growth. World Journal of Agricultural Science 3: 350-355.

Tandon, H.L.S. 1989. Fertilizer management in food crops. Fertilizer development and consultation organization. Journal of New Delhi India, p. 191-198.

Van Othman, W.M., Lio, T.A., Mannetje, L., and Wassink, G.Y. 1991. Low level phosphorus supply affecting nodulation, N2 fixation and growth of cowpea (Vigna unguiculata L. Walp). Plant and Soil 135: 67-74.

Vessy, K. 2003. Plant growth promoting rhizobacteria as biofertilizars. Plant and Soil 255: 571-586.

Wahyudi, A.T., and Astuti, R.I. 2011. Screening of pseudomonas sp. isolated from rhizosphere of soybean plant as plant growth promoter and bio-control agent. American Journal of Agricultural and Biological Sciences 6(1): 134- 141.

Waling, I., W.V. Vark, V.J.G. Houba, and J.J. Vanderlee. 1989. Soil and plant analysis, a series of syllabi. Part 7. Plant analysis procedures. Wageningen Agriculture University, the Netherland. P. 263.

Waluyo, S., Tek, H., Lie, and Mannetje, L. 2004. Effect of phosphate on nodule primordia of soybean (Glycine max Merrill) in acid soils in rhizotron experiments. Indonesian Journal of Agricultural Science 5: 37-44.

Yurtseven, E., Kesmez, G.D., and Unlukara, A. 2005. The effects of water salinity and potassium levels on yield, fruit quality and water consumption of a native central Anatolian tomato species (Lycopersicon esculentum). Agricultural Water Management 78: 128-135.

Zahir, A.Z., Arshad, M., and Frankenberger, W.F. 2004. Plant growth promoting rhizobacteria: applications and perspectives in agriculture. Advances in Agronomy 81: 97-168.

Zaidi, A., and Khan, M.S. 2005. Interactive effect of rhizospheric microorganisms on growth, yield and nutrient uptake of wheat. Journal of Plant Nutrition 28: 2079-2092.

Zeidan, M.S. 2007. Effect of organic manure and phosphorus fertilizers on growth, yield and quality of lentil plants in sandy soil. Agricultural Research and Biological Sciences 3(6): 748-752.

Zekri, M., and Obreza, A. 2003. Plant nutrients for citrus trees, Soil and Water Science Department, Institute of Food and Agricultural Sciences, University of Florida, fact sheet SL 200, January 2003.