Effect of barley (Hordeum vulgare L.) and Persian clover (Trifolium respinatum L.) intercropping on forage quality

Document Type : Scientific - Research

Authors

• Ramin Nazarian ¹
• Ali Reza Koocheki ¹
• Mahdi Nassiri Mahallati ¹
• Parviz Rezvani Moghaddam ²

¹ Ferdowsi Mashhad

² Ferdowsi mashhad

10.22067/jag.v11i2.45216

Abstract

Introduction
Forage quality is limited in many forage production systems in developing countries, specifically in arid and semiarid conditions. Mixing Persian clover as a forage legume with cereals such as barley has been proposed as a way of increasing forage quantity and quality. Barley (Hordeum vulgare L.) is an annual cereal which can grow in marginal area, suppress weed pressure and provide high forage yield but protein content of the forage is low. Intercropping cereals and legumes have been suggested to increase forage nutrient .stated that barley intercropped with Berseem clover (Trifolium alexandrinum L.) compared with oat or triticale (Tritico×secale rimpaui Wittm.) produced higher protein in forage production. In addition, Lithourgidis et al. (2006) has been reported that vetch (Vicia sativa L.) intercropped with oat produced higher total protein compared with a sole stand of vetch. Carr et al. (2004) demonstrated that barley forage compared with oat (Avena sativa L.) had higher dry matter digestibility (DMD), lower acid detergent fiber (ADF), and higher crude protein (CP). In current study we compared a wide range of cropping rate and pattern to determine the best forage quality in mixture of barley and Persian clover in a low-input system.
 
Materials and methods
In order to find the optimum cropping rate and pattern in a mixture of barley and Persian clover an experiment was conducted in a split plot layout based on randomized complete block design with three replications at Research Farm of College of Agriculture, Ferdowsi University of Mashhad in 2013-14. The pattern of sowing considered in five levels (row intercropping 1:1, sowing one row barley one row clover (M1), row intercropping 2:2, sowing two row barley two row clover (M2), strip intercropping 3:3, sowing three row barley three row clover (M3), strip intercropping 4:4, sowing four row barley four row clover (M4) and mixed cropping barley and clover (M5)) which allocated as main plots and the cropping rate in five levels (additive mixture of barley %100: %50 clover (R5), additive mixture of barley %100: %25 clover (R4), replacement mixture of barley %50: %50 clover (R3), with pure barley (R2)and pure clover (R1)) proposed as subplots. Barley (Watan local variety originated from Herat, Afghanistan) and Persian clover (Trifolium respinatum L.) seed rates were considered 120 kg.ha-1 and 40 kg.ha-1, respectively. Sowing date was on October 23. Barley was harvested in heading stage and clover in completed flowering stage. Two random samples (0.5-1 kg) were chose in each plot to determine forage fresh yield and quality characteristics. Samples were dried at 75ºC for 72 h, then milled and determined percentage of forage dry matter. The forage quality characteristics examined were dry matter digestibility (DMD), organic matter digestibility (OMD), digestive value (D-value), crude protein (CP), protein yield (PY), neutral detergent fiber (NDF) and acid detergent fiber (ADF). The pepsin-cellulase In vitro two step method was used for determine DMD, OMD and D-value. Crud protein; NDF and ADF contents were determine based on Nelson et al. (1973) and  Goering and Van Soest (1970) methods, respectively. Analysis of variance (ANOVA) and lest significant different test (LSD) were performed using SAS version 9.3 (SAS Institute Inc., Cary, NC, USA).
Results and Discussion
Results showed that the effect of cropping rate and pattern on DMD, OMD, D-value and protein yield (PY) were significant (p<0.01).  The highest levels of DMD, OMD and D-value were obtained in M1 sowing pattern and R1 pure clover, respectively .The effect of sowing pattern on CP, NDF and ADF contents were not significant. The highest CP content was shown in pure clover (16.3 %) and the highest PY were obtained in pure barley (1962.2 kg.ha-1) and mixed cropping (1584.4 kg.ha-1). So producing high DMD, OMD, D-value and CP in clover and PY in barley alone is not sufficient but must also consider the stability of the system that it is implemented in mixed cultures.

Keywords

• Dry matter digestibility (DMD)
• Organic matter digestibility (OMD)
• Digestive value (D-value)

20.1001.1.20087713.1398.11.2.11.8

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References

Anil, L., Park, R.H., Phipps, R.H., and Miller, F.A. 1998. Temperate intercropping of cereals of cereals for forage: a review of the potential for growth and utilization with particular reference to the UK. Grass Forage Science 53: 301–317.

Broderick, G.A., Walgenbach, R.P., and Malgnan, S. 2001. Production of lactating dairy cows fed alfalfa or red clover at equal dry matter or crude protein contents in the diet. Journal of Dairy Science 84: 1728–1737.

Broderick, G.A., Brito, A.F., and Olmos Colmenero, J.J. 2007. Effects of feeding formate-treated alfalfa silage or red clover silage on the production of lactating dairy cows. Journal of Dairy Science 1378–1391.

Banik, P., Midya, A., Sarkar, B.K., and Gose, S.S. 2006. Wheat and chickpea intercropping system in an additive series experiment: advantages and weed smothering. European Journal of Agronomy 24: 325- 332.

Banik, P., Sasmal, T., Ghosal, P.K., and Bagchi, D.K. 2000. Evaluation of mustard (Brassica campestris var Toria) and legume intercropping under 1:1 and 2:1 row replacement series systems. Journal of Agronomy and Crop Science. 185: 9–14.

Buxton, D. R., and Brasche, M. R. 1991. Digestibility of structural carbohydrates in cool-season grass and legume forages. Crop Science 31:1338–1345.

Buxton, D. R and Russell, J. R. 1988. Lignin constituents and cell-wall digestibility of grass and legume stems. Crop Science 28: 553–558.

Brundage, A.L. 1969. Nutritive value of oat and pea components of a forage mixture harvested sequentially. Journal of Dairy Science 53: 793–796.

Caballero, R., Goicoechea, E.L. and Hernaiz, P.J. 1995. Forage yields and quality of common vetch and oat sown at varying seeding ratios and seeding rates of common vetch. Field Crops Research 41: 135–140.

Carr, P.M., Martin, G.B., Caton, J.S. and Poland, W.W. 1998. Forage and nitrogen yield of barley–pea and oat–pea intercrops. Agronomy Journal 90: 79–84.

Chapko, L.B. 1991. Oat, oat–pea, barley, and barley–pea for forage yield, forage quality, and alfalfa establishment. Journal of Production. Agriculture 4: 486–491.

Chen, C., Westcott, M., Neill, K., Wichman, D., and Knox, M. 2004. Row configuration and nitrogen application for barley–pea intercropping in Montana. Agronomy Journal 96: 1730–1738.

Clements, R.O., and Donaldson, G. 1997. Clover and cereal: low input bi-cropping. Farming Conserve 3: 12–14.

Dhima, K.V., Lithourgidis, A.S., Vasilakoglou, I.B. and Dordas, C.A. 2007. Competition indices of common vetch and cereal intercrops in two seeding ratio. Field Crops Reserches 100: 249-256.

Dordas, C.A., and Lithourgidis, A.S. 2011. Growth, yield and nitrogen performance of faba bean intercrops with oat and triticale at varying seeding ratios. Grass Forage Science 66: 569–577.

Exner, D.N., and Cruse, R.M. 1993. Inter seeded forage legume potential as winter ground cover, nitrogen source, and competition. Journal of Production Agriculture 6: 226-231.

Engels, F.M., and Jung, H.G. 1998. Alfalfa stem tissues: Cell-wall development and lignification. Annals of Botany 82: 561-568.

Goering, H.K., and Van Soest, P.J. 1970. Forage fiber, analyses (apparatus, reagents, procedures, and some applications). Agriculture Hand Book 379, USDA. P. 1-20.

Herbert, S.J., Putnam, D.H., Poos-Floyd, M.L., Vargas, A., and Creighton, J.F. 1984. Forage yield of intercropped corn and soybean in various planting patterns. Agronomy Journal 76: 507–510.

Javanmard, A., Nasab, A.D.M., Javanshir, A., Moghaddam M., and Janmohammadi, H. 2009. Forage yield and quality in intercropping of maize with different legumes as double-cropped. Journal of Food Agriculture Environment. 7: 163-166.

Jeyabal, A., and Kuppuswamy, G. 2001. Recycling of organic wastes for the production of vermin compost and its response in rice-legume cropping system and soil fertility. European Journal of Agronomy 15: 153-170.

Jones, L., and Clements, R.O. 1993. Development of a low-input system for growing wheat (Triticum vulgare) in a permanent understory of white clover (Trifolium repens). Annual Apply Biology 123: 109–119.

Jung, H.G., and Deetz, D.A. 1993. Cell wall lignification and degradability. In: Jung H.G, Buxton D.R, Hatfield R.D. and Ralph, J. Forage cell wall structure and digestibility. Madison, WI, USA: American Society of Agronomy 315–346.

Lithourgidis, A.S., Vasilakoglou, I.B., Dhima, K.V., Dordas, C.A., and Yiakoulaki, M.D. 2006. Forage yield and quality of common vetch mixtures with oat and triticale in two seeding ratios. Field Crops Reserches 99: 106–113.

Lithourgidis, A.S., and Dordas, C.A. 2010. Forage yield, growth rate and nitrogen uptake of wheat, barley and rye–faba bean intercrops in three seeding ratios. Crop Science 50: 2148–2158.

Mohsenabadi, G.R., Jahansooz, M.R., Chaichi, M.R., Mashhadi, H.R., Liaghat, A.M., and Savaghebi, G.R. 2008. Evaluation of barley vetch intercrop at different nitrogen rates. Journal of Agriculture Science Technology 10: 23–31.

Nelson, D.W., and Sommers, L.E. 1973. Determination of total nitrogen in plant material. Agronomy Journal 65: 109-112

Ross, S.M., King, J.R., O’Donovan, J.T., and Spaner, D. 2004a. Forage potential of intercropping berseem clover with barley, oat, or triticale. Agronomy Journal 96: 1013–1020.

Saedi nejad, A., Rezvani moqaddam, P., Khazaie, H. and Nasirie mahallaty, M., 2011. Effect of organic matter, biological and chemical fertilizer application on sorghum protein content and digestibility in Spidfed variety. Journal of Iran agronomy research. 9 (4): 623 - 630

Sadeghpour, A., and Jahanzad, E. 2012. Seed yield and yield components of intercropped barley (Hordeum vulgare L.) and annual medic (Medicago scutellata L.). Australian Journal of Agriculture Engineering 3: 47–50.

Sadeghpour. A., Jahanzada, E., Esmaeili, A., Hosseini, M.B., and Hashemi, M. 2013. Forage yield, quality and economic benefit of intercropped barley and annual medic in semi-arid conditions: Additive series. Field Crops Research 148: 43–48.

Sistach, M. 1990. Intercropping of forage sorghum, maize and soybean during ten establishments of different grasses in a vertisol soil. Cuban Journal of Agriculture Science 24: 123 – 129.

Strydhorst, S.M., King, J.R., Lopetinksy, K.J., and Harker, K.N. 2008. Forage potential of intercropping barley with faba bean, lupin, or field pea. Agronomy Journal 100: 182–190.

Thorsted, M.D., Koefoed, N., and Olesen, J.E. 2002. Intercropping of oats (Avena sativa L.) with different white clover (Trifolium repens L.) cultivars. Effects on biomass development and oat yield. Journal of Agriculture Science Cambridge 138: 261–267.

Vasilakoglou, I. and Dhima, K., 2008. Forage yield and competition indices of berseem clover intercropped with barley. Agronomy Journal. 100, 1749–1756.

Wiersma, D.W., Smith, R.R., Mlynarek, M.J., Rand, R.E., Sharpee, D.K., and Undersander, D.J. 1998. Harvest management effects on red clover forage yield, quality, and persistence. Journal of Production Agriculture 11: 309–313.