Development of a Critical Nitrogen Dilution Curve Based on Leaf Dry Matter for Two Spring Rapeseed (Brassica napus L.) Cultivars

Siadat, Seyed Ataollah; Derakhshan, Abolfazl

doi:10.22067/jag.v11i4.72921

Development of a Critical Nitrogen Dilution Curve Based on Leaf Dry Matter for Two Spring Rapeseed (Brassica napus L.) Cultivars

Document Type : Scientific - Research

Authors

Department of Plant Production and Genetics, Agriculture faculty, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.

10.22067/jag.v11i4.72921

Abstract

Introduction
Supplementing crop growth with nitrogen (N) can significantly increase yields. However, the excessive use of N in intensive cropping systems has led to lower N use efficiency, an increase in greenhouse gas emissions as well as water pollution. The critical N concentration (Nc) is the minimum N required for maximal growth and can be used as a tool for accurate N management during the growing season. Leaf dry matter (LDM) is an important indicator of crop growth potential and a measure of light-energy utilization, and yield formation in rapeseed. LDM increases as more N fertilizer is applied, although differences in LDM are small under high fertilization levels. Therefore, construction of a Nc curve based on LDM during the vegetative growth of spring rapeseed would be a worthwhile objective. Our aims in the present study were (i) to construct a leaf Nc curve, (ii) to compare this curve with published data, and (iii) to explore its potential for estimating spring rapeseed leaf N status.
Materials and Methods
A field experiment with seven levels of N fertilizer including 0, 50, 100, 150, 200, 250 and 300 kg.ha-1 was performed, and LDM and leaf N concentration (LNC) of two spring rapeseed cultivars were measured during the growing season. The procedure used for constructing the leaf Nc curve was first proposed by Justes et al. (1994). The N nutrition index (NNI) on each sampling date was calculated by dividing LNC by leaf Nc concentration. The accumulated N deficit (Nand) in leaves for each sampling date was determined by subtracting the N accumulation in leaves under the Nc condition from actual N accumulation in leaves under different N rates.
Results and Discussion
N application rate exhibited a significant effect on LDM during the vegetative growth of rapeseed. The LDM increased gradually with increasing N utilization. LDM ranged from 0.06-3.83 ton.ha-1 for Hyola 401 cv. and from 0.06-3.56 ton.ha-1 for Dalgan cv. LNC generally increased with increasing N application. LNC remained relatively stable during the early growth but decreased gradually as cultivars reached the flowering stage. LNC values ranged from 2.44-6.2% for Hyola 401 cv. and from 3.14-6.1% for Dalgan cv. The leaf Nc concentrations in Hyola 401 and Dalgan cultivars declined with increasing LDM and the curve for these cultivars was fitted according to the equation Nc=5.08LDM-0.06. The similar trends of decline in leaf N dilution have been previously reported in winter wheat, maize and rice, when N dilution was estimated on the whole plant or specific plant organs basis. NNI values began to decrease with the decrease in the quantity of applied N. NNI values varied from 0.72-1.14 in the Dalgan cv. and from 0.53-1.15 in the Hyola 401 cv. The NNI values were greater than one for non-N limiting treatments and less than one for N-limiting treatments. These results confirm that NNI can provide accurate and quantitative insight into the N nutrition status of rapeseed. The Nand values ranged between -11.61-107.09 kg N ha-1 in the Dalgan cv. and between -24.22-129.64 for the Hyola 401 cv. depending on the N dosage. Nand decreased with increased N rates, while intensified gradually over growth progress of rapeseed and reached its peak at flowering stage for N-limiting treatments. These results confirmed the usefulness of Nand for assessing N nutrition in spring rapeseed. There was a significant positive correlation between the changes in N application rates (ΔN) and the changes in NNI (ΔNNI), and between ΔN and the changes in Nand (ΔNand). Generally, the Nc dilution curve, NNI, and Nand derived from it well recognized nutrition status of two cultivars under N-limiting and non-N limiting conditions, and can be used to as a reliable indicator of the crop N status during the growing season.
Conclusion
The N concentration in the canopy leaves decreased with advancing maturity, while a higher N application rate generally exhibited a higher leaf N concentration. The present Nc dilution curve based on LDM provides an insight into N nutrition status in spring rapeseed plant and can serve as a novel tool to improve N fertilization management in rapeseed.

Materials and Methods: A field experiment with seven levels of N fertilizer including 0, 50, 100, 150, 200, 250 and 300 kg.ha-1 was performed, and LDM and leaf N concentration (LNC) of two spring rapeseed cultivars were measured during the growing season. The procedure used for constructing the leaf Nc curve was first proposed by Justes et al. (1994). The N nutrition index (NNI) on each sampling date was calculated by dividing LNC by leaf Nc concentration. The accumulated N deficit (Nand) in leaves for each sampling date was determined by subtracting the N accumulation in leaves under the Nc condition from actual N accumulation in leaves under different N rates.
Results and Discussion: N application rate exhibited a significant effect on LDM during the vegetative growth of rapeseed. The LDM increased gradually with increasing N utilization. LDM ranged from 0.06-3.83 ton.ha-1 for Hyola 401 cv. and from 0.06-3.56 ton.ha-1 for Dalgan cv. LNC generally increased with increasing N application. LNC remained relatively stable during the early growth but decreased gradually as cultivars reached the flowering stage. LNC values ranged from 2.44-6.2% for Hyola 401 cv. and from 3.14-6.1% for Dalgan cv. The leaf Nc concentrations in Hyola 401 and Dalgan cultivars declined with increasing LDM and the curve for these cultivars was fitted according to the equation Nc=5.08LDM-0.06. The similar trends of decline in leaf N dilution have been previously reported in winter wheat, maize and rice, when N dilution was estimated on the whole plant or specific plant organs basis. NNI values began to decrease with the decrease in the quantity of applied N. NNI values varied from 0.72-1.14 in the Dalgan cv. and from 0.53-1.15 in the Hyola 401 cv. The NNI values were greater than one for non-N limiting treatments and less than one for N-limiting treatments. These results confirm that NNI can provide accurate and quantitative insight into the N nutrition status of rapeseed. The Nand values ranged between -11.61-107.09 kg N ha-1 in the Dalgan cv. and between -24.22-129.64 for the Hyola 401 cv. depending on the N dosage. Nand decreased with increased N rates, while intensified gradually over growth progress of rapeseed and reached its peak at flowering stage for N-limiting treatments. These results confirmed the usefulness of Nand for assessing N nutrition in spring rapeseed. There was a significant positive correlation between the changes in N application rates (ΔN) and the changes in NNI (ΔNNI), and between ΔN and the changes in Nand (ΔNand). Generally, the Nc dilution curve, NNI, and Nand derived from it well recognized nutrition status of two cultivars under N-limiting and non-N limiting conditions, and can be used to as a reliable indicator of the crop N status during the growing season.
Conclusions: The N concentration in the canopy leaves decreased with advancing maturity, while a higher N application rate generally exhibited a higher leaf N concentration. The present Nc dilution curve based on LDM provides an insight into N nutrition status in spring rapeseed plant and can serve as a novel tool to improve N fertilization management in rapeseed.

Keywords

20.1001.1.20087713.1398.11.4.10.1

References

Ata-Ul-Karim, S.T., Xia, Y., Liu, X.J., Cao, W.X., and Zhu, Y., 2013. Development of critical nitrogen dilution curve of Japonica rice in Yangtze River Reaches. Field Crops Research 149: 149–158.

Ata-Ul-Karim, S.T., Cao, Q., Zhu, Y., Tang, L., Rehmani, M.I.A., and Cao, W., 2016. Nondestructive assessment of plant nitrogen parameters using leaf chlorophyll measurements in rice. Frontiers in Plant Science 7: 1829.

Ata-Ul-Karim, S.T., Zhu, Y., Lu, X.J., Cao, Q., Tian, Y.C., and Cao, W., 2017a. Estimation of nitrogen fertilizer requirement for rice crop using critical nitrogen dilution curve. Field Crops Research 201: 32–40.

Ata-Ul-Karim, S.T., Liu, X., Lu, Z., Zheng, H., Cao, W., and Zhu, Y., 2017b. Comparison of different critical nitrogen dilution curves for nitrogen diagnosis in rice. Scientific Reports 7: 42679.

Bremner, J.M., and Mulvancy, C.S., 1982. Nitrogen-total. In: A.L. Page (Ed.). Methods of Soil Analysis, Part 2. American Society of Agronomy, Madison, WI. p. 595–624.

Colnenne, C., Meynard, J.M., Reau, R., Justes, E., and Merrien, A., 1998. Determination of a critical nitrogen dilution curve for winter oilseed rape. Annals of Botany 81: 311–317.

Fitzgerald, G., Rodriguez, D., and O’Leary, G., 2010. Measuring and predicting canopy nitrogen nutrition in wheat using a spectral index-the canopy chlorophyll content index (CCCI). Field Crops Research 116: 318–324.

Gayler, S., Wang, E., Priesack, E., Schaaf, T., and Maidl, F.X., 2002. Modeling biomass growth, N uptake and physiological development of potato crop. Geoderma 105: 367–383.

Greenwood, D.J., Lemaire, G., Gosse, G., Cruz, P., Draycott, A., and Neeteson, J.J., 1990. Decline in percentage N of C3 and C4 crops with increasing plant mass. Annals of Botany 66: 425–436.

Justes, E., Mary, B., and Machet, J.M., 1994. Determination of a critical nitrogen dilution curve for winter wheat crops. Annals of Botany 74: 397–407.

Lemaire, G., and Gastal, F., 1997. Nitrogen uptake and distribution in plant canopie. In G. Lemaire (Ed.). Diagnosis of the Nitrogen Status in Crops. Springer-Verlag Publishers, Heidelberg. p. 3–43.

Lemaire, G., van Oosterom, E., Sheehy, J., Jeuffroy, M.H., Massignam, A., and Rossato, L., 2007. Is crop demand more closely related to dry matter accumulation or leaf area expansion during vegetative growth? Field Crops Research 100: 91–106.

Miranzadeh, M.B., Heidari, M., Dehghan, S., and Hasanzadeh, M., 2010. An overview of nitrate in drinking water and its health effect with emphasis on its carcinogenic risk in human. Health System Research 6: 1057–1071. (In Persian with English Summary)

Rico-Garcia, E., Hernandez, F., Soto-Zarazua, G., and Herrera-Ruiz, G., 2009. Two new methods for the estimation of leaf area using digital photography. International Journal of Agriculture and Biology 11: 397–400.

Schlemmer, M., Gitelson, A., Schepers, J., Ferguson, R., Peng, Y., Shanahan, J., and Rundquist, D., 2013. Remote estimation of nitrogen and chlorophyll contents in maize at leaf and canopy levels. International Journal of Applied Earth Observation and Geoinformation 25: 47–54.

Sinclair, T.R., and Horie, T., 1989. Leaf nitrogen, photosynthesis and crop radiation use efficiency: a review. Crop Science 29: 90–98.

Weymann, W., Sieling, K., and Kage, H., 2016. Organ-specific approaches describing crop growth of winter oilseed rape under optimal and N-limited conditions. European Journal of Agronomy 82: 71–79.

Xu, G.H., Fang, X.R., and Miller, A.J., 2012. Plant nitrogen assimilation and use efficiency. Annual Review of Plant Biology 63: 153–182.

Yao, X., Ata-Ul-Karim, S.T., Zhu, Y., Tian, Y., Liu, X., and Cao, W., 2014a. Development of critical nitrogen dilution curve in rice based on leaf dry matter. European Journal of Agronomy 55: 20–28.

Yao, X., Zhao, B., Tian, Y.C., Liu, X.J., Ni, J., Cao, W.X., and Zhu, Y., 2014b. Using leaf dry matter to quantify the critical nitrogen dilution curve for winter wheat cultivated in eastern China. Field Crops Research 159: 33–42.

Yue, S.C., Meng, Q.F., Zhao, R.F., Li, F., Chen, X.P., Zhang, F.S., and Cui, Z.L., 2012. Critical nitrogen dilution curve for optimizing nitrogen management of winter wheat production in the North China Plain. Agronomy Journal 104: 523–529.

Zhao, B., Ata-Ul-Karim, S.T., Liu, Z., Ning, D., Xiao, J., Liu, Z., Qin, A., Nan, J., and Duan, A., 2017. Development of a critical nitrogen dilution curve based on leaf dry matter for summer maize. Field Crops Research 208: 60–68.

Zhao, B., Liu, Z.D., Ata-Ul-Karim, S.T., Xiao, J.F., Liu, Z.G., and Qi, A.Z., 2016. Rapid and nondestructive estimation of the nitrogen nutrition index in winter barley using chlorophyll measurements. Field Crops Research 185: 59–68.

Zhao, S., Qiu, S., Cao, C., Zheng, C., Zhou, W., and He, P., 2014. Responses of soil properties, microbial community and crop yields to various rates of nitrogen fertilization in a wheat-maize cropping system in north-central China. Agriculture, Ecosystems and Environment 194: 29–37.

Zhao, B., 2014. Determining of a critical dilution curve for plant nitrogen concentration in winter barley. Field Crops Research 160: 224–234.

Ziadi, N., Belanger, G., Claessens, A., Lefebvre, L., Cambouris, A.N., Tremblay, N., Nolin, M.C., and Parent, L.É., 2010. Determination of a critical nitrogen dilution curve for spring wheat. Agronomy Journal 102: 241–250.

Ziadi, N., Brassard, M., Belanger, G., Claessens, A., Tremblay, N., Cambouris, A., Nolin, M., and Parent, L. E., 2008. Chlorophyll measurements and nitrogen nutrition index for the evaluation of corn nitrogen status. Agronomy Journal 100: 271–273.