تأثـیر کودهای آلی بر ترکیب و عملکرد اسانس در کشت پایدار گیاه دارویی زوفا (Hyssopus officinalis L.)

نوع مقاله : علمی - پژوهشی

نویسندگان

1 گروه زراعت، دانشکده کشاورزی، واحد رودهن، دانشگاه آزاد اسلامی، رودهن، ایران

2 گروه زراعت، دانشکده کشاورزی، واحد رودهن، دانشگاه آزاد اسلامی، رودهن، ایران.

چکیده

به‌منظور بررسی اثر کمپوست، ورمی­کمپوست و باکتری­های تثبیت‌کننده نیتروژن بر ترکیب اسانس در کشت پایدار گیاه دارویی زوفا، آزمایشی در قالب طرح پایه بلوک­های کامل تصادفی در هشت تیمار و سه تکرار در مزرعه تحقیقاتی شرکت کشاورزی ران در شهرستان فیروزکوه در سال 1391 انجام گرفت. تیمارهای آزمایش شامل 20 تن کمپوست در هکتار، 12 تن ورمی­کمپوست در هکتار، کود زیستی (تلقیح بذر با ازتوباکتر و آزوسپیریلوم)، 10 تن کمپوست + 6 تن ورمی­کمپوست در هکتار، 20 تن کمپوست در هکتار + کود زیستی، 12 تن ورمی­کمپوست در هکتار + کود زیستی، 10 تن کمپوست + 6 تن ورمی­کمپوست + کود زیستی و شاهد (عدم مصرف کود) بودند. صفات مورد مطالعه شامل عملکرد اندام رویشی و درصد، عملکرد و ترکیبات اسانس بودند. استخراج اسانس توسط روش تقطیر با آب و به‌کمک دستگاه کلونجر انجام شد و ترکیبات اسانس به‌وسیله گاز کروماتوگرافی و گاز کروماتوگرافی با طیف‌سنج جرمی تعیین شدند. تجزیه واریانس توسط نرم‌افزار SAS و مقایسه میانگین­ها به‌وسیله آزمون حداقل اختلاف معنی­دار (LSD) در سطح احتمال پنج درصد انجام گرفت. نتایج حاصل از این آزمایش نشان داد که بیشترین عملکرد اندام­های رویشی (6/2601 کیلوگرم در هکتار)، درصد اسانس (200/0 درصد) و عملکرد اسانس (21/5 کیلوگرم در هکتار) در مصرف تلفیقی 12 تن ورمی­کمپوست و کود زیستی (2 لیتر در هکتار) به‌دست آمد. بیشترین درصد سیس­پینوکامفون (56/63%) و درصد بتافلاندرن (71/9%) و کمترین درصد ترانس­پینوکامفون (50/2%) در اسانس در مصرف تلفیقی 10 تن کمپوست، 6 تن ورمی­کمپوست و کود زیستی و بیشترین درصد والریانول (36/5%) و درصد فیتول (19/2%) در اسانس در کاربرد 20 تن کمپوست حاصل گردید. در مجموع بیشترین کمیت اسانس با کاربرد تلفیقی 12 تن ورمی­کمپوست و کود زیستی و بیشترین کیفیت اسانس با مصرف تلفیقی 10 تن کمپوست، 6 تن ورمی­کمپوست و کود زیستی حاصل شد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effects of Organic Fertilizers on Essential Oil Composition and Yield in Sustainable Cultivation of Hyssop (Hyssopus officinalis L.)

نویسندگان [English]

  • Mohammad Taghi Darzi 1
  • Bijan Sadeghi Nekoo 2
  • Mohammad Reza Haj Seyed Hadi 2
1 Department of Agronomy, Faculty of Agriculture, Roudehen Branch, Islamic Azad University, Roudehen, Iran.
2 Department of Agronomy, Faculty of Agriculture, Roudehen Branch, Islamic Azad University, Roudehen, Iran.
چکیده [English]

Introduction
Organic fertilizers such as compost and vermicompost improve soil's physical and chemical properties that enhance beneficial microbe's activity in soil and nutrient availability by plants. Nitrogen-fixing bacteria such as Azotobacter and Azospirillum as biofertilizers also have the ability of nitrogen-fixing and release phytohormones which could stimulate plant growth, absorption of nutrients, and the photosynthesis process. Production of medicinal plants with using from biofertilizers leads to an increase of the quantity and quality of active substances such as essential oil. Hyssop (Hyssopus officinalis L.) is a perennial herb that grows wild and is cultivated in temperate regions of Asia, Europe, and America. The essential oil of hyssop is widely used as a traditional drug in some the parts of Asia and Europe to treat respiratory diseases. Hyssop is also used in food, pharmaceutical, flavor, and cosmetic industries throughout the world. The main constituents of hyssop essential oil have been reported as pinocamphone, pinocarvone, and phellandrene.
 Materials and Methods
In order to study the effects of compost, vermicompost, and nitrogen-fixing bacteria as sustainable nutrient management on the essential oil composition of Hyssopus officinalis, an experiment was conducted based on a randomized complete block design with eight treatments and three replications at the research field of Agriculture Company of Ran in Firouzkooh of Iran during 2012. The treatments were 20 t.ha-1 compost, 12 t.ha-1 vermicompost, biofertilizer (inoculated seeds with Azotobacter chroococcum and Azospirillum lipoferum), 10 t.ha-1 compost  + 6 t.ha-1 vermicompost,  20 t.ha-1 compost + biofertilizer, 12 t.ha-1 vermicompost  + biofertilizer, 10 t.ha-1 compost + 6 t.ha-1 vermicompost + biofertilizer and control (without fertilizer). Studied traits were contained herb yield and percent, yield, and composition of essential oil. The essential oil extraction was done by the hydro-distillation method in Clevenger’s apparatus, and essential oil composition was determined by gas chromatography (GC) and gas chromatography-mass spectrometry (GC/MS). Analysis of variance was conducted by SAS software, and the means were compared by the least significant difference test (LSD) at the 5% probability level.
Results and Discussion
Results showed that the highest herb yield (2601.6 kg.ha-1), essential oil percent (0.20%), and essential oil yield (5.21 kg.ha-1) were obtained in integrated application of 12 t.ha-1 vermicomposts and biofertilizer (nitrogen-fixing bacteria)(2 lit/ha). The maximum cis Pinocamphone percent (63.56%) and beta Phellandrene percentage (9.71%) and the minimum trans Pinocamphone percent (2.50%) in an essential oil by integrated using of 10 t.ha-1 compost, six t.ha-1 vermicomposts, and biofertilizer and the highest Valerianol percentage (5.36%) and Phytol percentage (2.19%) in essential oil with the application of  20 t.ha-1 compost were obtained.        
Integrated application of vermicompost and biofertilizer through the high ability for absorption of mineral nutrients like N, P, and K by plants and then increase of biomass amount has a positive effect on essential oil content. Increased essential oil yield in treatment of the integrated application of vermicompost (12 t.ha-1) and biofertilizer can be owing to the improvement of yield attributes such as; essential oil percentage and herb yield. Some studies have reported that the application of bio and organic fertilizers can increase the content and yield of essential oil in a few medicinal plants such as basil, chamomile, and coriander. Integrated application of bio and organic fertilizers through the improvement of biological activities of soil and nutrient elements absorption such as nitrogen and phosphorus, caused more growth and biomass production, which leads to improvement of the essential oil quality. Several studies have shown a beneficial effect on the essential oil composition of medicinal plants by the integrated application of bio and organic fertilizers.  
Conclusion
Generally, the highest essential oil quantity with the integrated application of 12 t.ha-1 vermicomposts and biofertilizer and the highest essential oil quality with the integrated application of 10 t.ha-1 compost, six t.ha-1 vermicomposts and biofertilizer were obtained.

کلیدواژه‌ها [English]

  • Biofertilizer
  • Cis pinocamphone
  • Compost
  • Vermicompost
Adams, R.P., 2007. Identification of essential oil components by gas chromatography/mass spectrometry, Fourth ed. Allured Publishing Corporation, Illinois.
Akbari, I., and Gholami, A., 2016. Evaluation of mycorrhizal fungi, vermicompost and humic acid on essence yield and root colonization of fennel. Iranian Journal of Field Crops Research 13: 840-853. (In Persian with English Summary)
Akbarinia, A., Ghalavand, A., Sefidkon, F., Rezaee, M.B., and Sharifi Ashoorabadi, E., 2004. Study on the effect of different rates of chemical fertilizer ,manure and mixture of them on seed yield and main, compositions of essential oil of Ajowan (Trachyspermum copticum). Pajouhesh and Sazandegi 61: 32-41. (In Persian with English Summary)
Anwar, M., Patra, D.D., Chand, S., Alpesh, K., Naqvi, A.A., and Khanuja, S.P.S., 2005. Effect of organic manures and inorganic fertilizer on growth, herb and oil yield, nutrient accumulation, and oil quality of French basil. Communications in Soil Science and Plant Analysis 36: 1737-1746.
Arancon, N., Edwards, C.A., Bierman, P., Welch, C., and Metzger, J.D., 2004. Influences of vermicomposts on field strawberries: 1. Effects on growth and yields. Bioresource Technology 93: 145-153.
Asheghi, P., Darzi, M.T., and Haj Seyed Hadi, M.R., 2018. Effects of organic and bio-fertilizers on biomass yield and quantity and quality of essential oil of green basil (Ocimum basilicum L.) in Firouzkuh region. Iranian Journal of Horticultural Science 49: 199-211. (In Persian with English Summary)
Bahadori, F., and Ghorbanian, D., 2018. Evaluation of vermicompost and arbuscular mycorrhizal fungi (Glomus intraradices) efficiency on essential oil and dry herb yield of Thymus vulgaris in the field conditions. Plant Productions Technology 18: 81-91. (In Persian with English Summary)
Darzi, M.T., Haj Seyed Hadi, M.R., and Rejali, F., 2013. Effects of vermicompost and phosphatic biofertilizer application on quantity and quality of essential oil in anise (Pimpinella anisum L.). Iranian Journal of Medicinal and Aromatic Plants 29: 583-594. (In Persian with English Summary)
Darzi, M.T., and Haj Seyed Hadi, M.R., 2014. Response of concentration and composition of essential oil of coriander (Coriandrum sativum L.) to cattle manure and nitrogen fixing bacteria. Ethno-Pharmaceutical Products 1: 35-42.
Darzi, M.T., and Haj Seyed Hadi, M.R., 2017. The effects of manure, vermicompost, nitroxin and bio-superphosphat application on quantity and quality of essential oil of dragonhead. Crops Improvement 19: 543-560. (In Persian with English Summary)
Darzi, M.T., and Miri, H., 2018. Effects of manure and phosphate solubilizing biofertilizer on growth, yield and essential oil quality of dragonhead (Dracocephalum moldavica L.) in Firouzkuh region. Iranian Journal of Field Crop Science 49: 35-45. (In Persian with English Summary)
Darzi, M.T., and Mojtabavi, K., 2018. Effects of vermicompost and nitroxin application on flower yield, yield components and essential oil quality of marigold (Calendula officinalis L.). Iranian Journal of Medicinal and Aromatic Plants 33: 1034-1046. (In Persian with English Summary)
Ebadi M.T., Azizi, M., Sefidkon, F., and Ahmadi, N., 2016. Effects of organic and chemical fertilizers on leaf yield, percent and essential oil components of Lippia citriodora Kunth. Journal of Horticultural Science 30: 293-302. (In Persian with English Summary)
El Ghadban, E.A.E., Shalan, M.N., and Abdel, L.T.A.T., 2006. Influence of biofertilizers on growth, volatile oil yieldand constituents of fennel (Foeniculum vulgare Mill.). Egyptian Journal of Agricultural Research 84: 977-992.
Gholami Ganje, S., Salehi, A., Shahrasbi, S., and Amirinasab, A.M., 2019. Evaluation the effect of vermicompost different levels and mycorrhiza fungi on yield and essential oil production of Anise (Pimpinella anisum L.). Iranian Journal of Horticultural Science 50: 325-335. (In Persian with English Summary)
Gholami Sharafkhaneh, E., Jahan, M., Banayan Avval, M., Koocheki, A.R., and Rezvani Moghaddam, P., 2015. The effect of organic, bio and chemical biofertilizers on some agroecological characteristics, yield and essential oil of Satureja hortensis L. in Mashhad conditions. Journal of Agroecology 7: 179-189. (In Persian with English Summary)
Govahi, M., Ghalavand, A., Nadjafi, F., and Sorooshzadeh, A., 2016. Effects of different soil fertility systems on some physiological characteristics, yield and essential oil of sage (Salvia officinalis L.) under different irrigation regimes. Iranian Journal of Medicinal and Aromatic Plants 32: 333-345. (In Persian with English Summary)
Govahi, M., Ghalavand, A., Nadjafi, F., and Sorooshzadeh, A., 2017. Effects of vermicompst, bio and nitrogen fertilizers on quantitative and qualitative yield of sage (Salvia officinalis L.) under different humidity condition. Journal of Agroecology 9: 445-457. (In Persian with English Summary)
Haj Ali Araghi, M., Darzi, M.T., and Haj Seyed Hadi, M.R., 2019. Integrated application of vermicompost and nitroxin and quantitative and qualitative characteristics of germanchamomile (Matricaria chamomilla L.). Journal of Agriculture Science and Sustainable Production 29: 85-99. (In Persian with English Summary)
Hussein, M.S., El-Sherbeny, S.E., Khalil, M.Y., Naguib, N.Y., and Aly, S.M., 2006. Growth characters and chemical constituents of Dracocephalum moldavica L. plants in relation to compost fertilizer and planting distance. Scientia Horticulturae 108: 322-331.
Kamayestani, N., Rezvani Moghaddam, P., Jahan, M., and Rejali, F., 2015. Effects of separated and integrated application of bio and organic fertilizers on some quantitative and qualitative characteristics of anise (Pimpinella anisum L.). Iranian Journal of Field Crops Research 13: 62-70. (In Persian with English Summary)
Kapoor, R., Giri, B., and Mukerji, K.G., 2004. Improved growth and essential oil yield and quality in Foeniculum vulgare Mill on mycorrhizal inoculation supplemented with P-fertilizer. Bioresource Technology 93: 307-311.
Khalesro, S., and Malekian, M., 2017. Effects of vermicompost and humic acid on morphological traits, yield, essential oil content and component in organic farming of ajwan (Trachyspermum ammi L.). Iranian Journal of Medicinal and Aromatic Plants 32: 968-980. (In Persian with English Summary)
Khalid, K.H.A., Hendawy, S.F., and El Gezawy, E., 2006. Ocimum basilicum L. production under organic farming. Research Journal of Agriculture and Biological Sciecnes 2: 25-32.
Kizil, S., Hasimi, N., Tolan, V., Kilinc, E., and Karatas, H., 2010. Chemical composition, antimicrobial and antioxidant activities of hyssop (Hyssopus officinalis L.) essential oil. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 38: 99-103.
Loomis, W.D., and Corteau, R., 1972. Essential oil biosynthesis. Recently Advance in Phytochemistry 6: 147-185.
Mahfouz, S.A., and Sharaf Eldin, M.A., 2007. Effect of mineral vs. biofertilizer on growth, yield, and essential oil content of fennel (Foeniculum vulgare Mill). International Agrophisics 21: 361-366.
Moghtader, M., 2014. Comparative evaluation of the essential oil composition from the leaves and flowers of Hyssopus officinalis L. Journal of Horticulture and Forestry 6: 1-5.
Mohammadi, H., and Rezaei Chiyaneh, E., 2019. Effect of vermicompost application on seed yield and quality in fababean (Vicia faba L.) and fennel (Foeniculum vulgare L.) intercropping. Iranian Journal of Crop Sciences 21: 139-154. (In Persian with English Summary)
Mohammadpour Vashvaei, R., Galavi, M., Ramroodi, M., and Fakheri, B.A., 2015. Effects of drought stress and biofertilizers inoculation on growth, yield and essential oil compositions of thyme (Thymus vulgaris L.). Journal of Agroecology 7: 237-253. (In Persian with English Summary)
Mojtabavi, K., and Darzi, M.T., 2018. Effects of vermicompost and nitroxin application on flower yield, yield components and essential oil quality of marigold (Calendula officinalis L.). Iranian Journal of Medicinal and Aromatic Plants 33: 1034-1046. (In Persian with English Summary)
Moradi, R., Nassiri Mahallati, M., Rezvani Moghaddam, P., Lakzian, A., and Nejad Ali, A., 2011. The effect of application of organic and biological fertilizers on quantity and quality of essential oil in fennel (Foeniculum vulgare). Journal of Horticultural Science 25: 25-33. (In Persian with English Summary)
Moro, A., Zalacain, A., De Mendoza, J.H., and Carmona, M., 2011. Effects of agronomic practices on volatile composition of Hyssopus officinalis L. essential oils. Molecules 16: 4131-4139.
Naguib, N.Y., 2003. Impact of mineral nitrogen fertilizer and organic compost on growth, herb and chemical composition of german chamomile (Chamomilla recutita L.). Egyptian Journal of Applied Science 18: 301–323.
Omidbaigi, R., 2000. Production and Processing of Medicinal Plants. Volume 3, Behnashr Co. 397 p. (In Persian)
Ormeno, E., and Fernandez, C., 2012. Effect of soil nutrient on production and diversity of volatile terpenoids from plants. Current Bioactive Compounds 8: 71-79.
Pandey, V., Verma, R.S., Chauhan, A., and Tiwari, R., 2014. Compositional variation in the leaf, flower and stem essential oils of hyssop (Hyssopus officinalis L.) from western-himalaya. Journal of Herbal Medicine 4: 89-95.
Rahmanian, M., Esmaielpour, B., Hadian, J., Shahriari, M.H., and Fatemi, H., 2017. The Effect of organic fertilizers on morphological traits, essential oil content and components of basil (Ocimum basilicum L.). Journal of Agriculture Science and Sustainable Production 27: 103-118. (In Persian with English Summary)
Razipour, P., Golchin, A., and Daghestani, M., 2016. Effects of different levels of cow manure and inoculation with nitroxin on growth and performance of Melissa officinalis L. Iranian Journal of Medicinal and Aromatic Plants 32: 807-823. (In Persian with English Summary)
Rezaei Chiyaneh, E., Faridvand, S., Amirnia, R., Mahdavikia, H., and Rahimi, A., 2019. Effect of organic and biofertilizers on yield and some qualitative characteristics of the dragon's head (Lallemantia iberica) in dryfarming conditions. Journal of Agriculture Science and Sustainable Production 28: 25-40. (In Persian with English Summary)
Rezvani Moghaddam, P., Amin Ghafouri, A., Bakhshaei, S., and Jafari, L., 2013. Effects of bio and organic fertilizers on some quantitative traits and essential oil content of summer savory (Satureja hortensis L.). Journal of Agroecology 5: 105-112. (In Persian with English Summary)
Salehi Surmeghi, M.H., 2008. Medicinal Plants and Phytotherapy. Volume 2, Doniaye Taghzieh. 377 p. (In Persian)
Sefidkon, F., 2001. Evaluation of qualitative and quantitative essential oil fennel (Foeniculum vulgare Mill) in different stages of growth. Iranian Journal of Medicinal and Aromatic Plants 7: 85-104. (In Persian with English Summary)
Sharma, A.K., 2002. Biofertilizers for Sustainable Agriculture. Agrobios, India, 407 p.
Soleymany, F., and Pirzad, A.R., 2016. The effect of mycorrhizal fungi on the oxidant enzymes activity in the medicinal herb, hyssop, under water deficit conditions. Iranian Journal of Medicinal and Aromatic Plants 31: 1013-1023. (In Persian with English Summary)Yousefzadeh, S., Modarres-Sanavy, S.A.M., Sefidkon, F., Asgarzadeh, A., Ghalavand, A., Roshdi, M., and Safaralizadeh, A., 2013. Effect of biofertilizer, azocompost and nitrogen on morphologic traits and essential oil content of Dracocephalum moldavica L. in two regions of Iran. Iranian Journal of Medicinal and Aromatic Plants 29: 438-459. (In Persian with English Summary)
Yousefzadeh, S., 2018. Effects of vermicompost levels application on some agromorphological traits, photosynthetic pigments and essential oil content of hyssop (Hyssopus officinalis L.). Journal of Crop Production 11: 131-137. (In Persian with English Summary)
Zheljazkov, V.D., Astatkie, T., and Hristov, A.N., 2012. Lavender and hyssop productivity, oil content, and bioactivity as a function of harvest time and drying. Industrial Crops and Products 36: 222-228.
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