تأثیر قارچ تریکودرما Trichoderma longibrachiatum و محلول‌پاشی کیتوزان بر ویژگی‌های مورفوفیزیولوژیک و عملکرد ریحان (Ocimum basilicum L.) در شرایط کم‌آبیاری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشگاه علوم کشاورزی و منابع طبیعی ساری، ایران

2 گروه زراعت، پژوهشکده ژنتیک و زیست‌فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران.

3 گروه زراعت، دانشکده علوم زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ایران

4 دانشگاه صنعتی شاهرود، ایران

چکیده

کم­آبی از مهم‌ترین عوامل تنش­زای محیطی است که تولید محصولات کشاورزی را به‌ویژه در مناطق خشک و نیمه‌خشک تحت تأثیر قرار داده و باعث کاهش عملکرد می­شود. بهره­گیری از ریزجانداران افزاینده رشد گیاه و استفاده از پلیمرهای زیستی مانند کیتوزان یکی از راهکارهای بهبود تحمل گیاه در شرایط کم­آبیاری پیشنهاد شده است. در همین راستا، پژوهشی به‌منظور بررسی تأثیر قارچTrichoderma longibrachiatum  و محلول­پاشی کیتوزان بر ویژگی‌های مورفوفیزیولوژیک و عملکرد ریحان (Ocimum basilicum L.) در شرایط کم­آبیاری، به­صورت کرت­های خرد شده- فاکتوریل در قالب طرح بلوک­های کامل تصادفی با سه تکرار اجرا شد. عامل­های آزمایش شامل کم­آبیاری در سه سطح (دور آبیاری دو روز به‌عنوان آبیاری نرمال و سه و چهار روز به‌عنوان شرایط کم­آبیاری) به‌عنوان عامل اصلی و کیتوزان در سه سطح (0، 2/0 و 4/0 گرم در لیتر) و قارچ T. longibrachiatum   در دو سطح شاهد و پیش­تیمار با قارچ تریکودرما به‌صورت فاکتوریل در کرت­های فرعی قرار گرفتند. نتایج نشان داد که با افزایش دور آبیاری از دو به چهار روز، صفات مورفولوژیک ریحان، مانند طول ریشه افزایش و طول ساقه، وزن خشک برگ، ریشه، ساقه و عملکرد ماده خشک کاهش یافت. همچنین، صفات فیزیولوژیک ریحان مانند کاروتنوئید و عدد کلروفیل­متر افزایش و مقدار کلروفیل a، b و کلروفیل کل کاهش یافت. در مقایسه، کاربرد سطح 2/0 کیتوزان در شرایط تلقیح با قارچ سبب افزایش محتوای کلروفیل b به‌میزان 68 درصد شد. همچنین، بالاترین درصد و عملکرد اسانس در هر دو شرایط آبیاری نرمال و کم­آبیاری با کاربرد قارچ تریکودرما و کیتوزان حاصل شد. در مجموع، یافته­ها بیانگر اثر مثبت کاربرد همزمان قارچ تریکودرما و کیتوزان در بهبود رﺷﺪ روﯾﺸﯽ و افزایش تحمل به تنش کم­آبیاری در ﮔﯿﺎه رﯾﺤﺎن ﺑﻮد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of Trichoderma longibrachiatum and Chitosan Spraying on Morphophysiological Characteristics and Yield of Sweet Basil (Ocimum basilicum L.) under Deficit Irrigation Conditions

نویسندگان [English]

  • Mahtab Miri 1
  • Hemmatollah Pirdashti 2
  • Arastoo Abbasian 3
  • Zahra Nouri Akandi 4
  • Mehranoosh Emamian Tabarestani 1
1 Sari Agricultural Sciences and Natural Resources University, Sari, Iran.
2 Professor, Genetics and Agricultural Biotechnology Institute of Tabarestan, Department of Agronomy, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.
3 Department of Agronomy, Faculty of Crop Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
4 Shahroud University of Technology, shahroud, Iran
چکیده [English]

Introduction
 Drought is one of the most important environmental stressors that adversely affects agricultural products, especially in arid and semi-arid regions. Using Trichoderma fungus along with biopolymers such as chitosan is one of the ways to reduce drought stress. Trichoderma fungus as plant growth-promoting fungus is the most common fungal and soil-modifying species that are able to directly with plant roots in the rhizosphere and improve growth as well as biological control of living stresses such as pathogenic fungi and non-living stresses such as drought, salinity and heavy metals. On the other hand, one of the effective ways to protect the plant in conditions of low irrigation is the use of anti-transpirants, including the biostimulant of chitosan, which markedly limits transpiration from the plant surface. The anti-transipirants action of chitosan can be attributed to the involvement of chitosan in the abscisic acid pathways, which closes the stomata and thus reduces transpiration. Chitosan is readily soluble in water and organic acids. Therefore, it can be used in various methods such as mixing with soil, foliar spraying and impregnation with seeds in agriculture.
Material and Methods
 This research was conducted in a split factorial arrangement based on randomized complete block design. The main plot factor was irrigation interval in three levels (two days as normal irrigation and three and four days as deficit irrigation conditions) and sub-plots were inoculated with T. longibrachiatum at two levels (inoculation and uninoculated control) and chitosan at three levels (0, 0.2 and 0.4 g/L). Each experimental plot consisted of three planting lines two and a half meters long and one meter wide .T. longibrachiatum was obtained from Tabarestan Agricultural Genetics and Biotechnology Research Institute. The first irrigation was done simultaneously with planting basil. Up to one month after sowing the seeds (six to eight leaf stage of plants), the plots were irrigated evenly with tubes and from this stage onwards, irrigation treatments were applied. Pesticides and herbicides were not used during the experiment and weed control was done manually. Chitosan was prepared from Sarina Teb store and prepared in three levels of zero, 0.2 and 0.4 g/l and sprayed in three stages: vegetative, before flowering and 50% flowering.
Results and Discussion
 The results showed that with increasing the irrigation period from two to four days, the morphological traits of basil, such as root length and stem length, leaf dry weight, root, stem and dry matter yield decreased. Also, physiological traits of basil such as carotenoids and chlorophyll meter increased while chlorophyll a, b and total chlorophyll decreased. Application of 0.2 g/L of chitosan inoculated plants increased chlorophyll b content by 68%. The highest percentage and yield of essential oil in both normal and irrigation deficit conditions were obtained when plants inoculated with Trichoderma and foliary sprayed by chitosan. The highest percentage and yield of essential oil were observed with an average of 0.88 and 42.87% in normal irrigation conditions, application of Trichoderma and zero level of chitosan, respectively. According to the results, increasing the irrigation cycle along with chitosan application and fungal inoculation increased the percentage and yield of essential oil. However, by increasing the irrigation cycle, chitosan alone decreased the percentage and yield of essential oil and only in the three-day irrigation cycle, it increased the percentage of essential oil compared to the control.
Conclusion
 Overall, the findings showed the positive effect of concomitant use of Trichoderma fungus and chitosan on improving the growth of sweet basil and increasing drought resistance.
Acknowledgements
Thanks and appreciation from the financial support provided by the Department of Agronomy and Plant Breeding Engineering, Sari Agricultural Sciences and the Natural Resources University of Sari, Iran.
 

کلیدواژه‌ها [English]

  • Essential oils
  • sweet basil
  • dry weight
  • photosynthetic pigments
  • plant growthpromoting microorganisms
  1. Abdel-Mawgoud, A.M.R., Tantawy, A.S., El-Nemr, M.A., & Sassine, Y.N. (2010). Growth and yield responses of strawberry plants to chitosan application. European Journal of Scientific Research39(1), 170-177.
  2. Abdel-Monaim, M.F. (2013). Improvement of biocontrol of damping-off and root rot/wilt of faba bean by salicylic acid and hydrogen peroxide. Mycobiology41(1), 47-55. https://doi.org/10.5941/MYCO.2013.41.1.47.
  3. Adams, R.P. (2017). Identification of essential oil components by gas chromatography/mass spectrometry. 5 online ed. Gruver, TX USA: Texensis Publishing.
  4. Agarwal, S., Sairam, R.K., Srivastava, G.C., & Meena, R.C. (2005). Changes in antioxidant enzymes activity and oxidative stress by abscisic acid and salicylic acid in wheat genotypes. Biologia Plantarum49, 541-550. https://doi.org/10.1007/s10535-005-0048-z
  5. Aghlmand, S., Poor Ismailpour, B., Jalilvand, P., Heidari, H.R., & Tavakoli Hassankloo, N. (2017). The effect of salicylic acid and paclobutrazol on growth and physiological traits of basil under water stress. Journal of Plant Process and Function, 6(19), 35-46. (In Persian with English Summary)
  6. Anith, K.N., Faseela, K.M., Archana, P.A., & Prathapan, K.D. (2011). Compatibility of Piriformospora indica and Trichoderma harzianum as dual inoculants in black pepper (Piper nigrum). Symbiosis55, 11-17. https://doi.org/10.1007/s13199-011-0143-1.
  7. Arriagada, C., Aranda, E., Sampedro, I., Garcia-Romera, I., & Ocampo, J.A. (2009). Contribution of the saprobic fungi Trametes versicolor and Trichoderma harzianum and the arbuscular mycorrhizal fungi Glomus deserticola and claroideum to arsenic tolerance of Eucalyptus globulusBioresource Technology100(24), 6250-6257. https://doi.org/10.1016/j.biortech.2009.07.010.
  8. Bae, H., Sicher, R.C., Kim, M.S., Kim, S.H., Strem, M.D., Melnick, R.L., & Bailey, B.A. (2009). The beneficial endophyte Trichoderma hamatum isolate DIS 219b promotes growth and delays the onset of the drought response in Theobroma cacao. Journal of Experimental Botany60(11), 3279-3295. https://doi.org/10.1093/jxb/erp165.
  9. Bazazi, N., Khodam Bashi, M., & Mohammadi, S. (2013). The effect of drought stress on morphological characteristics and yield components of fenugreek. Production and Processing of Agricultural and Horticultural Products, 11, 3-23. (In Persian with English Summary) https://doi.org/10.22092/ijmapr.2019.123365.2400.
  10. Boonlertnirun, S., Boonraung, C., & Suvanasara, R. (2008). Application of chitosan in rice production. Journal of Metals, Materials and Minerals18(2).
  11. Chibu, H., Shibayama, H., & Arima, S. (2002). Effects of chitosan application on the shoot growth of rice and soybean. Japanese Journal of Crop Science71(2), 206-211. https://doi.org/10.1626/jcs.71.206.
  12. Chmielewski, A.G., Migdal, W., Swietoslawski, J., Jakubaszek, U., & Tarnowski, T. (2007). Chemical-radiation degradation of natural oligoamino-polysaccharides for agricultural application. Radiation Physics and Chemistry76(11-12), 1840-1842. https://doi.org/10.1016/j.radphyschem.2007.04.013.
  13. Clarke, J.M., & McCaig, T.N. (1993). Breeding for efficient root systems. In Plant breeding: principles and prospects(pp. 485-499). Dordrecht: Springer Netherlands. https://doi.org/10.1007/978-94-011-1524-7_29.
  14. Doni, F., Isahak, A., Che Mohd Zain, C.R., & Wan Yusoff, W.M. (2014). Physiological and growth response of rice plants (Oryza sativa) to Trichoderma spp. inoculants. Amb Express4, 1-7. https://doi.org/10.1186/s13568-014-0045-8.
  15. Dutta, P.K., Dutta, J., & Tripathi, V.S. (2004). Chitin and chitosan: Chemistry, properties and applications.
  16. Dzung, N.A., Khanh, V.T.P., & Dzung, T.T. (2011). Research on impact of chitosan oligomers on biophysical characteristics, growth, development and drought resistance of coffee. Carbohydrate Polymers84(2), 751-755. https://doi.org/10.1016/j.carbpol.2010.07.066.
  17. El-Tantawy, E.M. (2009). Behavior of tomato plants as affected by spraying with chitosan and aminofort as natural stimulator substances under application of soil organic amendments. Pakistan Journal of Biological Sciences: PJBS12(17), 1164-1173. https://doi.org/10.3923/pjbs.2009.1164.1173.
  18. Emami Bistgani, Z., Siadat, S.A., Bakhshandeh, A., Pirbalouti, A.G., & Hashemi, M. (2017). Interactive effects of drought stress and chitosan application on physiological characteristics and essential oil yield of Thymus daenensisThe Crop Journal5(5), 407-415. https://doi.org/10.1016/j.cj.2017.04.003.
  19. Farooq, M., Wahid, A., Kobayashi, N.S.M.A., Fujita, D.B.S.M.A., & Basra, S.M.A. (2009). Plant drought stress: effects, mechanisms and management. Sustainable Agriculture, 153-188. https://doi.org/10.1007/978-90-481-2666-8_12.
  20. Gerami, M., Majidian, P., Ghorbanpour, A., & Alipour, Z. (2020). Stevia rebaudiana Bertoni responses to salt stress and chitosan elicitor. Physiology and Molecular Biology of Plants26(5), 965-974. https://doi.org/10.1007/s12298-020-00788-0.
  21. Ghoname, A.A., El-Nemr, M.A., Abdel-Mawgoud, A.M.R., & El-Tohamy, W.A. (2010). Enhancement of sweet pepper crop growth and production by application of biological, organic and nutritional solutions. Research Journal of Agriculture and Biological Sciences6(3), 349-355.
  22. Gupta, A.K., & Kaur, N. (2005). Sugar signalling and gene expression in relation to carbohydrate metabolism under abiotic stresses in plants. Journal of Biosciences30, 761-776. https://doi.org/10.1007/BF02703574.
  23. Hashem, A., Abd_Allah, E.F., Alqarawi, A.A., Al Huqail, A.A., & Egamberdieva, D. (2014). Alleviation of abiotic salt stress in Ochradenus baccatus (Del.) by Trichoderma hamatum (Bonord.) Bainier. Journal of Plant Interactions9(1), 857-868. https://doi.org/10.1080/17429145.2014.983568.
  24. Heng, Y., Frette, X.C., Christensen, L.P., & Grevsen, K. (2012). Chitosan oligosaccharides promote the content of polyphenols in Greek oregano (Origanum vulgare hirtum). Journal of Agricultural and Food Chemistry60(1), 136-143. https://doi.org/10.1021/jf204376j.
  25. Hidalgo, L., Argelles, W., Peniche, C., Pino, M., & Terry, E. (1996). Effect of chitosan in seed treatment of tomato. Revista de Protection Vegeta, 11(1), 37-39.
  26. Hirano, S. (1996). Chitin biotechnology applications. Biotechnology Annual Review2, 237-258. https://doi.org/10.1016/S1387-2656(08)70012-7.
  27. Hussaini Begum, M., Taheri, G.H., Vaezi Kakhaki, M.R., & Tlaty, M. (2013). Foliar application of chitosan on growth and morphological characteristics of marigold (Calendula officinalis). National Conference of Passive Defense in the Agricultural Sector, Ahvaz, Iran, 6 September 2013. (In Persian with English Summary).
  28. Iriti, M., & Faoro, F. (2009). Chitosan as a MAMP, searching for a PRR. Plant Signaling & Behavior4(1), 66-68. https://doi.org/10.4161/psb.4.1.7408.
  29. John, R.P., Tyagi, R.D., Prevost, D., Brar, S.K., Pouleur, S., & Surampalli, R.Y. (2010). Mycoparasitic Trichoderma viride as a biocontrol agent against Fusarium oxysporum sp. adzuki and Pythium arrhenomanes and as a growth promoter of soybean. Crop Protection29(12), 1452-1459. https://doi.org/10.1016/j.cropro.2010.08.004.
  30. Kaewchai, S., Soytong, K., & Hyde, K.D. (2009). Mycofungicides and fungal biofertilizers. Fungal Diversity, 38, 25-50.
  31. Kalantar Ahmadi, S.A., Ebadi, A., Jahanbakhsh, S., Daneshian, J., & Siadat, S.A. (2014). Effects of water stress and nitrogen on changes of some amino acids and pigments in canola. Bulletin of Environment, Pharmacology and Life Sciences, 4, 114-122. (In Persian with English Summary).
  32. Kapoor, R., Giri, B., & Mukerji, K.G. (2004). Improved growth and essential oil yield and quality in Foeniculum vulgare mill on mycorrhizal inoculation supplemented with P-fertilizer. Bioresource Technology93(3), 307-311. https://doi.org/10.1016/j.biortech.2003.10.028.
  33. Khajeh, H., & Naderi, S. (2014). The effect of chitosan on some antioxidant enzyme activity and biochemical traits of Melissa. Journal of Crop Science in Dry Areas, 1, 100-116. (In Persian with English Summary).
  34. Khalvandi, M., Amerian, M., Pirdashti, H., & Baradaran Firouzabadi, M., & Gholami, A. (2017). Effect of Piriformospora indica on the quantity of essential oil and some physiological traits of peppermint in salinity stress. Plant Biology, 9(32), 2-19. (In Persian with English Summary)
  35. Kiani, S. P., Maury, P., Sarrafi, A., & Grieu, P. (2008). QTL analysis of chlorophyll fluorescence parameters in sunflower (Helianthus annuus) under well-watered and water-stressed conditions. Plant Science175(4), 565-573. http://dx.doi.org/10.1016/j.plantsci.2008.06.002.
  36. Labra, M., Miele, M., Ledda, B., Grassi, F., Mazzei, M., & Sala, F. (2004). Morphological characterization, essential oil composition and DNA genotyping of Ocimum basilicum cultivars. Plant Science167(4), 725-731. https://doi.org/10.1016/j.plantsci.2004.04.026.
  37. Larcher, W. (2001). Physiological plant ecology. Springer verlage Berlin Heidelberg NewYork, Germany. 513 pp.
  38. Lashkari, F. (2013). The effect of polymer and superabsorbent, potassium and animal manure on quantitative and qualitative characteristics of Carla medicinal plant in different irrigation cycles. Master Thesis in Agriculture, Faculty of Agriculture, Zabol University, Iran. (In Persian with English Summary)
  39. Lawlor, D.W., & Cornic, G. (2002). Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants. Plant, cell & environment25(2), 275-294. https://doi.org/10.1046/j.0016-8025.2001.00814.x.
  40. Levitt, J. (1980). Responses of Plants to Environmental Stress, Volume 1: Chilling, Freezing, and High Temperature Stresses. Academic Press.497 pp.
  41. Limpanavech, P., Chaiyasuta, S., Vongpromek, R., Pichyangkura, R., Khunwasi, C., Chadchawan, S., & Bangyeekhun, T. (2008). Chitosan effects on floral production, gene expression, and anatomical changes in the Dendrobium orchid. Scientia Horticulturae116(1), 65-72. https://doi.org/10.1016/j.scienta.2007.10.034.
  42. Mahdavi,, Modares Sani, A.M., AghaAlikhani, M., & Sharifi, M. (2010). The effect of chitosan and water stress on morphological characteristics and root characteristics of safflower (Carthamus tinctorius L.). The 11th Congress of Agricultural Sciences and Plant Breeding, Tehran, Iran, 24 July 2010. (In Persian with English Summary)
  43. Malekpour, F., Salimi, A., & Ghasemi Pirblouti, A. (2015). The effect of chitosan biostimulant on physiological traits of purple basil under dehydration stress. Journal of Plant Ecophysiology 8(27), 71-56. (In Persian with English Summary).
  44. Mrkovacki, N., Marinković, J., Cacic, N., & Bjelic, D. (2010). Microbial abundance in rhizosphere of sugarbeet in dependance of fertilization and inoculation with azotobacter chroococcum. Research Journal of Agricultural Science42(3), 260-264.
  45. Mohammadi Kashka, F., Pirdashti, H., Yaghoubian, Y., & Bahari Sarvai, S. H. (2016). Coexistence of Trichoderma virens and Piriformospora indica with bacteria. Enterobacter sp on vegetative growth and photosynthetic pigments of pepper plant (Capsicum annuum). Scientific Journal of Plant Ecophysiology, 8(26),: 121-133. (In Persian with English Summary)
  46. Mohammadi, A., Ebrahimzadeh, H., Hadian, J., & Mir Masoumi, M. (2015.) Analysis of the effect of drought stress on some physiological and biochemical parameters of Lippia citriodora Journal of Plant Research, 28(3), 617-628. (In Persian with English Summary).
  47. Mondal, M.M., Malek, M.A., Puteh, A.B., Ismail, M.R., Ashrafuzzaman, M., & Naher, L. (2012). Effect of foliar application of chitosan on growth and yield in okra. Australian Journal of Crop Science6(5), 918-921.
  48. Morello, J.R., Romero, M.P., Ramo, T., & Motilva, M.J. (2005). Evaluation of L-phenylalanine ammonia-lyase activity and phenolic profile in olive drupe (Olea europaea) from fruit setting period to harvesting time. Plant Science168(1), 65-72. https://doi.org/10.1016/j.plantsci.2004.07.013.
  49. Navabpour, S., Ramezanpour, S.S., & Mazandarani, A. (2015). Evaluation of changes in enzymatic and non-enzymatic defense systems of soybean cultivars in response to drought stress during reproductive development. Bi-Quarterly Journal of Plant Production Technology, 7(2), 39-54. (In Persian with English Summary)
  50. Nouri Akandi, Z., Makarian, H., Pirdashti, H., Amerian, M.R., Firoozabadi Brothers, M., & Tajik Ghanbari, M.A. (2020). The effect of some symbiotic fungi and iron nanoparticles on morphological and physiological parameters of Portulaca oleracea under cadmium stress. Scientific Journal of Garden Nutrition, 3(1), 1-22. (In Persian with English Summary). https://doi.org/10.22070/hpn.2020.5242.1075.
  51. Porra, R.J. (2002). The chequered history of the development and use of simultaneous equations for the accurate determination of chlorophylls a and b. Photosynthesis Research73, 149-156. https://doi.org/10.1023/A:1020470224740.
  52. Ramos-Garcia, M., Ortega-Centeno, S., Hernández-Lauzardo, A.N., Alia-Tejacal, I., Bosquez-Molina, E., & Bautista-Baños, S. (2009). Response of gladiolus (Gladiolus spp) plants after exposure corms to chitosan and hot water treatments. Scientia Horticulturae121(4), 480-484. https://doi.org/10.1016/j.scienta.2009.03.002.
  53. Sajjadi, S.E. (2006). Analysis of the essential oils of two cultivated basils (Ocimum basilicum) from Iran. DARU Journal of Pharmaceutical Sciences14(3), 128-130.
  54. Salehi, S., & Rezayatmand, Z. (2017). The effect of foliar application of chitosan on yield and essential oil of savory (Saturejaisophylla) under salt stress. Journal of Medicinal Herbs,8(2), 101-108. https://doi.org/10.18869/JHD.2017.101.
  55. Salimi, G.H., Faizian, M., & Ali Asgharzad, N. (2020). The effect of mycorrhiza inoculation on nutrient uptake and essential oil components of Dracocephalum moldavica under drought stress. Scientific Journal of Crop Ecophysiology, 14(3): 325-344. (In Persian with English Summary)
  56. Sallam, N.M., Abo-Elyousr, K.A.M., & Hassan, M.A.E. (2008). Evaluation of Trichoderma species as biocontrol agents for damping-off and wilt diseases of Phaseolus vulgaris and efficacy of suggested formula. Egypt. J. Phytopathol36(1-2), 81-93.
  57. Sharma, P., Patel, A.N., Saini, M.K., & Deep, S. (2012). Field demonstration of Trichoderma harzianum as a plant growth promoter in wheat (Triticum aestivum L). Journal of Agricultural Science4(8), 65. https://doi.org/10.5539/jas.v4n8p65.
  58. Sheikha, S.A., & Al-Malki, F.M. (2011). Growth & chlorophyll responses of bean plants to the chitosan applications. European Journal of Scientific Research50(1), 124-134.
  59. Shukla, N., Awasthi, R.P., Rawat, L., & Kumar, J. (2012). Biochemical and physiological responses of rice (Oryza sativa) as influenced by Trichoderma harzianum under drought stress. Plant Physiology and Biochemistry54, 78-88. https://doi.org/10.1016/j.plaphy.2012.02.001.
  60. Sodaizadeh, H., Shamsai, M., Tajmalian, M., Mir Mohammadi Meybodi, S.A.M., & Hakimzadeh, M.A. (2016). The effect of drought stress on some morphological and physiological traits of savory (Satureja hortensis). Plant Process and Function, 5(15), 1-12. (In Persian with English Summary)
  61. Taghavi Ghasemkheili, F., Pirdashti, H., Tajik Qanbari, M.A., & Bahmaniar, M.A. (2014). The effect of Trichoderma harzianum and cadmium on barley tolerance and yield index (Hordeum vulgare). Journal of Crop Ecophysiology, 8(4), 465-482. (In Persian with English Summary)
  62. Taghinasab Darzi, M. (2012). The effect of some isolates of Trichoderma On increasing the growth of cucumber seedlings. Science and Technology of Greenhouse Cultivation, 1(3), 63-70. (In Persian with English Summary)
  63. Uthairatanakij, A., Teixeira da Silva, J.A., & Obsuwan, K. (2007). Chitosan for improving orchid production and quality. Orchid Science and Biotechnology1(1), 1-5.
  64. Vafaei Rostami, S., Abbasi, R., Pirdashti, H., & Qajar Spanloo, M. (2019). The effect of Piriformospora indica and Trichoderma harzianum on morphological traits and yield of peppermint essential oil (Mentha piperita) at different levels of phosphorus and irrigation. Journal of Agricultural Knowledge and Sustainable Production, 29(4), 37-50. (In Persian with English Summary).
  65. Vinale, F., Sivasithamparam, K., Ghisalberti, E.L., Marra, R., Woo, S.L., & Lorito, M. (2008). Trichoderma–plant–pathogen interactions. Soil Biology and Biochemistry40(1), 1-10. https://doi.org/10.1016/j.soilbio.2007.07.002.
  66. Vurayai, R., Emongor, V., & Moseki, B. (2011). Effect of water stress imposed at different growth and development stages on morphological traits and yield of Bambara Groundnuts (Vigna subterranea Verdc). American Journal of Plant Physiology6(1), 17-27.
  67. Waseem, S., Majeed, H., Naveed, I., Kayani, W.K., Ahmed, H., Hussain, S., & Kamal, A. (2010). Pharmacognostical study of the medicinal plant Calendula officinalis (family Compositae). International Journal of Cell & Molecular Biology1(2), 108-116.
  68. Willmer, C.M., & Pricker, M. (1996). Stomata. (2nd Edn), Chapman and Hall, London, 375 pp.
  69. Yagubian, Y., Pirdashti, H., Mohammadi GolTappeh, A., Faiziasl, V., & Esfandiari, A. (2012). Evaluation of dryland wheat (Triticum aestivum) Cultivar Azar 2 to coexistence with arbuscular and mycorrhizal fungi at different levels of drought stress. Journal of Agricultural Ecology, 4(1), 63-73. (In Persian with English Summary) https://doi.org/10.22067/jag.v4i1.14960.
  70. Yang, F., Hu, J., Li, J., Wu, X., & Qian, Y. (2009). Chitosan enhances leaf membrane stability and antioxidant enzyme activities in apple seedlings under drought stress. Plant Growth Regulation58, 131-136. https://doi.org/10.1007/s10725-009-9361-4
  71. Zeng, D., & Luo, X. (2012). Physiological effects of chitosan coating on wheat growth and activities of protectiveenzyme with drought tolerance. Journal of Soil Science, 2(3), 282-288. http://dx.doi.org/10.4236/ojss.2012.23034.
  72. Zhili, J., Yong, L., Juanjuan, L., Xu, X., Li, H., Lu, D., & Jingying, W. (2012). Effects of exogenous chitosan on physiological characteristics of potato seedlings under drought stress and rehydration. Potato Research, 55, 293-301. https://doi.org/10.1007/s11540-012-9223-8.
  73. Ziaei, M., Sharifi, M., Naqdi Badi, H., Academic, J., & Ghorbani Nahuji, M. (2014). A review of the medicinal plant basil (Ocimum basilicum) with emphasis on the main secondary compounds and its agronomic and medicinal properties. Quarterly Journal of Medicinal Plants, 13(4): 26-41. (In Persian with English Summary).

 

 

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