The Effects of Manure and Biological and Chemical Fertilizers on Yield and some Morphological Characteristics of Aloe vera (Aloe burbadensis Miller.) in Bushehr Province

Document Type : Scientific - Research

Authors

• Esmaeil Farrokhi ¹
• Ali Reza Koocheki ¹
• Mehdi Nassiri Mahallati ²
• Rahim Khademi ³

¹ Department of Agrotechnology, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran

² Department of Agrotechnology, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran.

³ Faculty of Agriculture and Natural Resources Research Center of Bushehr province, Iran

10.22067/jag.v1i1.35204

Abstract

Introduction
Aloe vera is a permanent, evergreen species with special industrial and pharmaceutical applications. However, information about its nutrition management is scarce. Plants absorb nutrients through complex interactions in the rhizosphere between roots, symbiotic or non-symbiotic microorganisms and soil fauna. Inoculations of soil microorganisms (in the form of biofertilizers) to improve crop production and plant health were mainly undertaken for the group of bacteria known as PGPB (plant growth-promoting bacteria) or PGPR (plant growth-promoting rhizobacteria) and mycorrhizal fungi. To be effective, inoculated organisms must have soil conditions that are suitable for development, which can be improved by applying manure. Compared to the use of inorganic fertilizers, the application of organic manure in agriculture fields results in significant effects on microbial biomass, on the profile of existing species and, consequently, on the enzymes they circulate in the soil and its pool of organic matter. In this way, it interacts with the overall fertility of the environment. Different options of soil fertility management for increasing yield of aloe vera were studied in a field experiment.
Materials and Methods
A split plot experimentwas conducted based on randomized complete block design with three replications during 2012-2013 growing season in Agricultural Research Center of Bushehr province (29º16'0"N 51º31'0"E and 110 m above sea level). Seedlings were planted on May 4, 2012 with a distance of 0.7 m spacing on row with 1 m distance. The distance between Aloe vera rows was 1 m. The drip irrigation method was used for irrigation.  Main plot factor consisted of 0 and 20 ton.ha^-1 sheep manure and five methods of soil fertility management were assigned to subplots including 1) chemical fertilizer (80 kg.ha^-1 super phosphate+200 kg.ha^-1 urea), 2) Mycorrhiza (using soil containing fungi from mosea), 3) biological fertilizer nitrogen (containing Azotobacter spp; Pseudomomas spp; and Azosprillium spp), 4) combination of Mycorrhiza and Nitrogen, and 5) no fertilizer (control). Monthly samplings were taken in 150, 180, 210, 240, 270 and 330 days after planting. Fresh and dry weight of leaves, leaf length, width and thickness and number of leaves per plant were measured. Harvestable leaves were considered as final yield.
Results and Discussion
The results indicated that interaction between manure and chemical or biological fertilizers was significant for all measured traits. Application of sheep manure was led to significantly higher growth and yield of aloe vera with better performance when manure was combined with chemical fertilizers. The highest number of leaves was obtained from integrated use of manure and chemical fertilizers with about 15 leaf per plant. While the lowest number of leaves was observed in control treatment. The interaction between treatments had a significant effect (p≤0.01) on leaf thickness. Plants treated with combination of chemical fertilizer and sheep manure had significant differences with other treatments, except with integrated use of biological fertilizers and manure. The highest fresh and dry weight of plant 3107.2 and 78.1 g plant^-1, respectively and the highest final yield (36.04 tons.ha^-1) was achieved with integrated use of manure and chemical fertilizers. The results also indicated that high aloe vera yields with great quality could be obtained with combined application of manure and biological fertilizers as an alternative for chemical fertilizers.
Conclusion
Producing medicinal plants under low input of chemical fertilizers is of great importance for human health, environment as well as production costs. Results of this experiment showed that application of manure with biofertilizers could be considered as a proper alternative to chemical fertilizers. Nutrition of aloe vera with this integrated management method will results to high yield and quality in addition to improving the physical, chemical and biological properties of soil.
Acknowledgment
We are grateful to Ebrahim Farrokhi and Kohzad Sartavi for their help with the field experiments, collecting field data and providing necessary advices to carry out this work.

Keywords

• Harvestable yield
• Sheep manure
• Mycorrhiza
• Nitrogen

20.1001.1.20087713.1400.13.3.3.6

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References

Adams, S.P, Leitch, I.J., Bennett, M.D., Chase, M.W., and Leitch A.R., 2000. Ribosomal
DNA evolution and phylogeny in Aloe (Asphodelaceae). Journal of Botany 87(11):1578-1583.

Aeini, M., Yousefi Rad, M., and Ehteshami, S.M., 2012. The effects of growth stimulant bacteria on qualitative and quantitative yield of Aloe vera. Annals of Biological Research 3(12): 5669-5673.

Akbari, P., Ghalavand, A., and Modarres Sanavi, S.A.M., 2009. Effects of different nutrition systems (organic, chemical and integrated) and biofertilizer on yield and othergrowth traits of sunflower (Helianthus annuus L.). Journal of sustainable agriculture 19(1): 83-93. (In Persian)

Akbarinia, A., Sefidcon, F., Rezaee, M.B., and Sharifi, A., 2003. Study on the effect of different rates of chemical fertilizer, manure and mixure of them on seed yield and main, compositions of essential oil of ajowan (Trachyspermum copticum). Pajouhesh and Sazandegi 6: 32-41. (In Persian with English Summary)

Allen, M.F., Swenson, W., and Querejeta, J.I., 2003. Egerton-Warburton L.M., Treseder K.K. Ecology of Mycorrhizae: A conceptual framework for complex interactions among plants and fungi. Annual Review of Phytopathology 41:271-303.

Allen, M.F., 1991. The Ecology of the Mycorrhizae. Cambridge University Press. p. 196 ISBN: 0521335531.

Al-Mario Casimir, J., and Morales-Payan, P., 2003. Growth and yield of cilantro fertilized with cow and sheep manures. 1^th International Hourticultural Congress: The Future for Medicinal and Aromatic Plants. Merto Toronto Convention Center. pp. 91.

Arancon, N., Edwards, C.A., Bierman, P., Welch, C., and Metzger, J.D., 2004. Influences of vermicomposts on field strawberries: 1. Effects on growth and yields. Bioresource Technology 93: 145-153.

Atiyeh, R.M., Arancon, N., Edwards, C.A., and Metzger, J.D., 2001. The influence of pig manure on the growth earthworm-processed and productivity of marigolds. Bioresource Technology 81: 103-108.

Baker., 2006. Nitrogen uptake characteristics of corn roots at low N concentration as influenced by plant age. Agronomy Journal 132: 17-19.

Barea, J.M., Pozo, M.J., Azcon, R., and Azcon-Aguilar, C., 2005. Microbial co-operation in the rhizosphere. Journal of Experimental Botany 56: 1761-1778.

Bauer, A., and Black, A.L., 1994. Quantification of the effect of soil organic matter content on soil productivity. Soil Science Society of America Journal 58: 185-193.

Copetta, A., Lingua, G., and Berta, G., 2006. Effects of three AM fungi on growth, distribution of glandular hairs, and essential oil production in Ocimum basilicum L. var. Genovese. Mycorrhiza 16: 485-494.

Dauden, A., and Quilez, D., 2004. Pig slurry vsrsus mineral fertilization on com yield and nitrate leaching in a Mediterranean irrigated environment. European Journal of Agronomy 21: 7-19.

Farrokhi, E., and Nassiri Mahallati, M., 2013. Situation of Aloe vera production in Bushehr province. Eighth Congress of Iranian Horticultural Science from p. 2982-1987. September 4^th to 7^th , Hamadan, Iran. (In Persian)

Ghalavand, A., Mohamadi, K., Agha Alikhani, M., Sohrabi, Y., and Haidari, G., 2012. Effects of different organic and biological fertilizers on yield and yield components of chickpea (Cicer aritenium L.). Agronomy Journal (Pajouhesh and Sazandegi) 94: 41-49. (In Persian)

Gryndler, M., Sudova, R., and Rydlova, J., 2008. Cultivation of high-biomass crops on mine spoil banks: Can microbial inoculation compensate for high doses of organic matter? Bioresource Technology 99: 6391–6399.

Hamman, J., 2008. Composition and applications of Aloe vera leaf Gel. Molecules 13(8): 1599-1616.

Harshavardhan, P.G., Vasundhara, M., Raviraja Shetty, G., Nataraja, A., Sreeramu, B.S., Chandre Gowda, M., and Sreenivasappa, K.N., 2007. Influence of spacing and integrated nutrient management on yield and quality of essential oil in lemon balm (Melissa officinalis L). Biomed 2(3): 288.

Hernández-Cruz, L.R., Rodriguez-García, R., Rodríguez, D.J., and Angulo-Sánchez, J.L., 2002. Aloe vera response to plastic mulch and nitrogen 570-574. In: Janick, J., and Whipkey, A., (Eds.). Trends in new crops and new uses. ASHS Press. 599 p.

Jahan, M., and Nassiri Mahallati, M., 2012. Soil Fertility and Biofertilizers (An Agroecological Approach). Ferdowsi University of Mashhad Press. Mashhad, Iran. No. 603. (In Persian)

Karami, A., and Sepehri, A., 2012. Integrated biological and chemical fertilizers on yield and borage (Borago officinalis L.) oil under water deficit stress. Iranian Journal of Field Crop Science 43(4): 691-699. (In Persian)

Kathuli, P., Nguluu, S.N., Musyoki, R., Omari, F., Matimbii, S.M., and Mutunga, R., 2010. Effects of fertilizer and manure application on growth and adaptability of three common aloe species in semi-arid eastern Kenya. Proceedings of 12^th KARI Biennial Conference held at KARI Hqts. 8-12 Nov. 2010. 1043-1048.

Kazi, L.H., 2007. Effect of different nitrogen and potassium rates on agronomic characters of Aloe indica. Building of Agronomy 35: 58-62.

Khoshbakht, T., Bahadori, f., Khalighi, A., and Ardalan, M.M., 2010. Effect of plant growth promoting bacteria on micro and macro elements and the Aloe vera plant in a greenhouse. Journal of Crop Physiology 8(2): 45-59. (In Persian)

Koocheki, A., and Khajeh Hosseini, M., 2008. Modern Agronomy. Jahad Mashhad University Press, Iran. 712 pages. (In Persian)

Krishna Moorthy, S., and Malliga, P., 2012. Plant characteristics, growth and leaf gel yield of Aloe barbadensis Miller. as affected by cyanopith biofertilizer in pot culture. International Journal of Civil and Structural Engineering 3(2): 884-892.

Lampkin, N., 1990. History of medicine. New York: Facts on file.

Makkizadeh, M., Nasrollahzadeh, S., Zehtab Salmasi, S., Chaichi, M., and Khavazi, K., 2011. The effect of organic, biologic and chemical fertilizers on quantitative and qualitative characteristics of sweet basil (Ocimum basilicum L.). Journal of Agricultural and Sustainable Production. 22(1): 1-12. (In Persian with English Summary)

Manjunatha, H.M., Sreeramu, B.S., Raviraja Shetty, G., Vasundhara, M., and Chandre Gowda, M., 2007. Influence of irrigation regimes and fertility levels on growth of long pepper (Piper longum L.). Biomed 2(3): 272.

Mirza, H., Kamal Uddin, A., Khalequzzaman, K.M., Shamsuzzaman, A.M.M., and Kamrun, N., 2008. Plant characteristics, growth and leaf yield of Aloe vera as affected by organic manure in pot culture. Australian Journal of Crop Science 2(3): 158-163.

Mooleki, S.P., Schoenau, J.J., Chales, J.L., and Wen, G., 2004. Effect of rat, frequency and incorporation of freedlot cattle manure on soil nitrogen availability, crop performance and nitrogen use efficiencyn in east-central Saskachwan. Canadian Journal of Soil Science 84: 199-210.

Morton, J. F., 1961. Folkuses and commercial exploitation of Aloe leaf pulp. Economic Botany 15: 311-319.

Mota-Fernández, S., Álvarez-Solis, J.D., Abud-Archila, M., Dendooven, L., and Gutiérrez-Miceli, F.A., 2011. Effect of arbuscular mycorrhizal fungi and phosphorus concentration on plant growth and phenols in micropropagated Aloe vera L. plantlets. Journal of Medicinal Plants Research 5(27): 6260-6266.

Nejatzadeh-Barandozi, F., Naghavi, M.R., Tahmasebi Enferadi, S., Mousavi, A., Mostofi, Y., and Hassani, M.E., 2012. Genetic diversity of accessions of Iranian Aloe vera based on horticultural traits and RAPD markers. Industrial Crops and Products 37: 347–351.

Omidbaigi, R., 2010. Production and Processing of Medicinal Plants. Astan Quds Razavi Publications (Behnaashr Co.), Iran. Volume foure. 400 pages. (In Persian)

Pouryousef, M., Mazaheri, D., Chaiechi, M.R., Rahimi, A., and Tavakoli, A., 2010. Effect of different soil fertilizing treatments on some of agro morphological traits and mucilage of isabgol (Plantago ovata Forsk). Electronic Journal of Crop Production 3(2): 193-213. (In Persian with English Summary)

Ramachandra, C.T., and Srinivasa, P., 2008. Processing of Aloe Vera gel: a review. American Journal Agricultural and Biological Science 3(2): 502–510.

Rodriguez-Garcia, R., Jasso de Rodriguez, D., and Angulo-Sanchez, J.L., 2000. Comparison between the production of leaves, gel and juice in Aloe vera grown with plastic mulch or natural conditions. In: Annual Meeting Association for the Advancement of Industrial Crops and New Uses Council, 15-17 October, St. Louis, Missouri, p. 35.

Rodriguez-Garcia, R., Jasso de Rodriguez, D., Gil-Marin, J.A., Angulo-Sanchez, J.L., and Lira-Saldivar, R.H., 2007. Growth, stomatal resistance, and transpiration of Aloe vera under different soil water potential. Industrial Crops and Products 25: 123–128.

Saha, R., Palit, S., Ghosh, B.C., and Mittra, B.N., 2005. Performance of Aloe vera as Influenced by Organic and Inorganic Sources of fertilizer supplied through fertigation. Conservation Cultivation and Sustainable Use of MAPs 2: 171-175.

Sharifi Ashour Abadi, E, and Abbas Zadeh, B., 2002. Effect of organic and chemical fertilizers on nitrogen uptake and efficacy of herbal fennel (Foeniculum vulgare Mill). Iranian Journal of Medical and Aromatic Plants 19(3): 313-324. (In Persian with English Summary)

Sharma, A.K., 2002. Biofertilizers for Sustainable Agriculture. Vol. 12, 319-324 pages. Agrobios, India.

Shoghi Kalkhoran, S., Ghalavand, A., Modares Sanavi, S.A.M., 2012. Effects of bio fertilizer and green manure (winter wheat) in combination with integrated nitrogen sources (chemical-farmyard manure) on quantitative and qualitative characteristics of sunflower (Helianthus annuus L.). Environmental Sciences 9(2): 35-52. (In Persian with English Summary)

Sartavi, k., and Gholamian, F., 2004. Medicinal plants of Bushehr province. Iranian Journal of Medicinal and Aromatic Plants Research 20(2): 213-227. (In Persian)

Uyanoz, R., Cetin, U., Zengin, M., and Gur, K., 2002. Effect of different organic wastes on nitro genmineralization and organic carbon contents of soil: International Conference on SustainableLand Use and Management, Canakkale, Turkey 2: 23-28.

Yazdani, D., Rezaei, M.B., Kyanbakht, S., and Khosravani, S., 2006. Overview of various aspects of medicinal Aloe vera (L.) Burm. f. Journal of Medicinal Plants 5(19):1-8. (In Persian with English Summary)

Zhao-Pu, L., Geng-Mao, Z., Ling, L., and Ging-Song, Z., 2006. Nitrogen metabolism of Aloe vera under long-term diluted seawater irrigation. Journal of Applied Horticulture 8(1): 33–36.

Ziaee, A.A., Mesgarpur, B., and Shabestary, A., 2005. Medicinal Plants: Evidence-based: Contraindications and Drug Interactions. Publications Teymurzade. 424 pages. Tehran, Iran. (In Persian with English Summary)