تأثیر کود گاوی و منابع مختلف تأمین‌کننده نیتروژن بر عملکرد و کارایی مصرف نیتروژن در گیاه گلرنگ (Carthamus tinctorius L.)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 رشته اگرواکولوژی، دانشکده کشاورزی، دانشگاه جیرفت، ایران.

2 گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه جیرفت، ایران

3 گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه جیرفت، ایران

4 گروه علوم خاک، دانشکده کشاورزی، دانشگاه جیرفت، ایران

چکیده

به‌منظور ارزیابی کود گاوی و منابع تأمین‌کننده نیتروژن بر جذب و کارایی مصرف نیتروژن در گیاه گلرنگ (Carthamus tinctorius L.)، آزمایشی به‌صورت فاکتوریل در قالب طرح بلوک‌های کامل تصادفی در سه تکرار در سال زراعی 98-1397 در مزرعه‌ تحقیقاتی دانشکده‌ کشاورزی دانشگاه جیرفت اجرا شد. تیمارها شامل منابع تأمین‌کننده  نیتروژن در شش سطح (کود اوره، اوره با پوشش گوگرد، نیترات آمونیوم، نیتروکسین، نانو نیتروژن و شاهد) و کود گاوی در دو سطح (صفر و 10 تن در هکتار) در نظر گرفته شد. نتایج نشان داد، تیمار کود گاوی و منابع مختلف کود نیتروژن بر عملکرد دانه، درصد نیتروژن دانه، درصد نیتروژن زیست‌توده و درصد نیتروژن کل بوته، محتوی نیتروژن اندام‌های مختلف گیاه، کارایی مصرف نیتروژن، کارایی فیزیولوژیک، کارایی جذب، بهره‌وری نیتروژن و شاخص برداشت نیتروژن گلرنگ معنی‌دار بود. بیشترین درصد نیتروژن دانه (46/3)، زیست‌توده (05/1)، عملکرد دانه ( 284 گرم در مترمربع)، محتوی نیتروژن دانه (8/1138 گرم در مترمربع) و محتوی نیتروژن زیست‌توده (5/752 گرم در مترمربع) در تیمار کود گاوی + کود نیتروکسین به‌دست آمد. همچنین، بیشترین کارایی مصرف نیتروژن (3/11 گرم بر گرم) و جذب نیتروژن (91/12 گرم بر گرم) در تیمار کود گاوی + کود نانو نیتروژن مشاهده شد که با تیمار کود گاوی + کود نیتروکسین تفاوت معنی‌داری نداشت. در بین منابع مختلف، نیتروژن مصرف کود گاوی و کودهای زیستی نسبت به کودهای شیمیایی شاخص بهره‌وری و کارایی مصرف نیتروژن بیشتری داشتند، از این‌رو بر اساس نتایج این پژوهش، جهت دستیابی به حداکثر عملکرد و کارایی مصرف نیتروژن و متعاقب آن کاهش آلودگی‌های زیست‌محیطی می‌توان از کود گاوی و کودهای نیتروکسین و نانو نیتروژن جهت کشت گلرنگ در منطقه جیرفت استفاده کرد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Evaluation of Cow Manure and Different Nitrogen Sources on Yield and Nitrogen Use Efficiency in Safflower (Carthamus tinctorius L.)

نویسندگان [English]

  • Roya Saeedi 1
  • Azam Seyyedi 2
  • Bahareh Parsa Motlagh 3
  • Hossein Shekofteh 4
1 Faculty of Agriculture, University of Jiroft, Jiroft, Iran University of Jiroft, Jiroft, Iran.
2 Department of Horticultural Science, Faculty of Agriculture, University of Jiroft, Jiroft, Iran
3 Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Jiroft, Jiroft, Iran.
4 Department of Soil Science, Faculty of Agriculture, University of Jiroft, Jiroft, Iran
چکیده [English]

Introduction
One of the major factors restricting the crop production is nitrogen. Nitrogen has an important role in achieving maximum yield and it improves the yield and quality of all crops (Ullah et al., 2010). In the arid and semi-arid regions of Iran, the deficiency of organic matter in the soil as the natural resource required by the plant and the moisture tension, are the major factors of absorbing nitrogen and after moisture tension, nitrogen tension is the major limiting factor of crop production (Sadri, 2017). Nitroxin fertilizer contains nitrogen fixing bacteria that fix the air nitrogen, balance the high-consumption and micronutrient elements, synthesis and secretion of plant growth stimulants and as a result, protection of plant against pathogens, biological and non-biological stresses.
One way to examine the efficiency of the fertilizers, especially nitrogen, is studying nitrogen use efficiency. This indicator shows the increase in yield by each unit increase in the input (Delbert and Ulter, 1989). One way to manage different nutritional resources is to evaluate the nitrogen use efficiency. This study aims to evaluate the function and indicators of nitrogen efficiency in manure treatments and different resources of nitrogen in safflower.
 
Material and Methods
This experiment was conducted as randomized complete block design with three replications in the research farm of faculty of agriculture university of Jiroft in 2018-2019 year. The first factor included different sources of nitrogen fertilizer in six levels of urea fertilizer, urea with sulfur coating, ammonium nitrate, nitroxin, nano nitrogen and control, the second factor included animal manure in two levels of consumption and non-consumption was considered. The intra row and inter row distance was 30 and 10 cm respectively with 2×3 m2 plot size. Drip irrigation was used and during the different plant growth stages, no chemical pesticide and herbicide was used nitrogen of seed, leaf and shoot was carried out using Kjeldhal method. Data were analyzed by SAS software version 9.4. Mean values were compared according to Duncan test at P < 0.05.
 
Results and Discussion
The results showed that manure and difference resource of nitrogen has significant effect on the seed yield, seed nitrogen percentage, biomass, plant nitrogen content, the efficiency of use, physiological, absorption, productivity and nitrogen harvest index in safflower. The highest nitrogen in seed (3.46%), biomass (1.05%), seed yield (284 g.m-2), seed nitrogen content (1138.8 g.m-2), and biomass nitrogen content (752.5 g.m-2) obtained in manure + nitroxin treatment. the results indicated the positive and significant effect of manure and biological fertilizer on the improvement of yield and nitrogen efficiency. The interaction of manure and different nitrogen fertilizers on seed yield was significant. The highest and lowest seed yield obtained by manure + nitroxin treatment (284 g.m-2) and not using manure and control treatment (68.3 g.m-2) respectively.
In safflower, using nitrogen fertilizers can increase seed yield by affecting the branches of the plant (Weiss, 2000). The researchers reported that the use of nitrogen, compared to control (not using nitrogen) increased safflower seed yield.
According to the results, manure treatment compared to not using manure treatment showed 24.3% increase in seed nitrogen. Nitroxin and nano nitrogen fertilizers have the highest seed nitrogen percentage (3.46 and 3.21, respectively) and the lowest nitrogen was in control treatment (1.9%). Manure + nitroxin treatment had the highest nitrogen in biomass and plant. In safflower, the use of manure increased the absorbed nitrogen percentage compared to control treatment (Ghanbari et al., 2016). In this regard, the researchers observed the increase in absorption efficiency and use of nitrogen in saffron through using organic fertilizers compared to chemical fertilizers. The results of this research showed that using biological fertilizers, especially nitroxin, has positive effect in increasing seed number in plant, seed yield, days to maturing, nitrogen percent, absorbed phosphorous by plant and nitrogen physiological efficiency (Arab-Niasar et al., 2019).
 
Conclusion
The results of this experiment showed that using manure and biological fertilizer improves nitrogen efficiency compared to not using manure condition. It seems that the combined use of manure with chemical fertilizers reduces the loss and washing nutritional elements, especially nitrogen. The use of organic fertilizers to improve soil structure, maintain water and access to required elements by plants should be considered as a priority. Regarding the highest yield in manure + nitroxin treatment, it is suggested to use nitrogen fertilizers with biological origin.

کلیدواژه‌ها [English]

  • Biofertilizer
  • Nitrogen harvest index
  • Nitrogen percentage
  • Nitrogen productivity
  1. Adediran, J.A., Taiwo, L.B., Akande, M.O., Sobulo, R.A., & Idowu, O.J. (2004). Application of organic and inorganic fertilizer for sustainable maize and cowpea yields in Nigeria. Journal of Plant Nutrition, 27, 1163-1181. https://doi.org/10.1081/PLN-120038542
  2. Agricultural Iran Statistics. (2019). https://www.maj.ir/Dorsapax/userfiles/Sub65/Amarnamehj3-1398-site.pdf.
  3. Akbari, H., & Modares Sanavy, S.A.M. (2019). Effects of Nutritional Management on Yield, Nitrogen Use Efficiency, Soil Organic Carbon and Nitrogen in Canola-Wheat Crop Rotation. Journal of Agroecology, 10(4), 1245-1257. https:// 22067/JAG.V10I4.63624.
  4. Arab-Niasar, L., Mirzakhani, M., & Nozad Namin, K. (2019). Evaluation of nitrogen efficiency and grain yield of white bean under combined application of organic and biological fertilizers. Journal of Agricultural Science and Sustainable Production, 29(3), 1-11. (In Persian with English Summary) https://sid.ir/paper/180593/fa
  5. Asadi, G.A., Momen, A., Nurzadeh Namaghi, M., & Khorramdel, S. (2014). Effects of organic and chemical fertilizer rates on nitrogen efficiency indices of isabgol (Plantago Ovata). Journal of Agroecoology, 5(4), 373-382. (In Persian with English Summary) https://sid.ir/paper/211303/fa
  6. Bayat Varkeshi, M., Hamid Zareabyaneh, H., & Mahdavi, S. (2017). Effect of nitrogen nanofertilizers and urea on yield and nitrogen efficiency in potatoes. Journal of Agroecology, 7(1), 81-95. (In Persian with English Summary) https://sid.ir/paper/254784/fa
  7. Burelle, N., Kloepper, J.W., & Reddy, M.S. (2006). Plant growth promoting rhizobacteria as transplant amendments and their effects on indigenous rhizosphere microorganisms. Applied Soil Ecology, 31, 91-100. https://doi.org/10.1016/j.apsoil.2005.03.007
  8. Chandra, K. (2005). Organic manures. Regional Centre of Organic Farming, 34, 6-46. https://rvskvv.net/images/Organic-Manures_20.04.2020.pdf
  9. Damodaram, T., & Hegde D.M. (2002). Oilseeds situation: a satisical compendium. Directorate of Oilseeds Research, Rajendranagar, Hyderabad India, 486 p.https://icar-iior.org.in/oilseeds-compendium
  10. Deibert, E.J., & Ulter, R.A. (1989). Sunflower growth and nutrient uptake: Response of tillage system, hybrid maturity and weed control method. Soil Science Journal, 53, 133-138. https://doi.org/10.2136/sssaj1989.03615995005300010025x
  11. Delogu, G., Cattivelli, L., Pecchioni, N., De Falcis, D., Maggiore, T., & Stanca, A.M. (1998). Uptake and agronomic efficiency of nitrogen in winter barley and winter wheat. European Journal of Agronomy, 9, 11–20. https://doi.org/10.1016/S1161-0301(98)00019-7
  12. Dordas, C.A., & Sioulas, C. (2008). Safflower yield, chlorophyll content, photosynthesis, and water use efficiency response to nitrogen fertilization under rain conditions. Field Crop Research, 27(1), 75-85. https://doi.org/10.1016/j.indcrop.2007.07.020
  13. Emongor, V.E., Oagile, O., & Kedikanetswe, B. (2013). Effects of plant population on growth, development and oil yield of safflower. Journal of Agricultural Science and Technology, 3, 321-333. http://:17660/ActaHortic.2015.1077.3
  14. FAO (Food and Agriculture Organization). (2018). FAOSTAT. http://www.fao.org/faostat /en/#data/QC
  15. Fathi, A., Farnia, A., & Maleki, A. (2013). Effects of nitrogen and phosphate biofertilizers on yield and yield components of corn AS71 in Dareh-shahr climate. Journal of Crop Ecophysiology, 7-1(25), 105-114. (In Persian with English Summary) https://sid.ir/paper/182741/fa
  16. Fathi, M., Mohebbi, M., & Koocheki, A. (2016). Introducing Prunus cerasus gum exudates: Chemical structure, molecular weight, and rheological properties. Food Hydrocolloids, 61, 946-955. https://doi.org/10.1016/j.foodhyd.2016.07.004
  17. Ghanbari, M., Mirzakhani, M., Faridhashmi, S. A. (2016). Physiological efficiency response of safflower nitrogen to the consumption of animal and chemical fertilizers in Kashan region. Journal of Plant Environmental Physiology, 11(41): 53-64. (In Persian).1001.1.76712423.1395.11.41.5.8
  18. Guarda, G., Padovan, S., & Delogu, G. (2004). Grain yield, nitrogen-use efficiency and baking quality of old and modern Italian bread-wheat cultivars grown at different nitrogen levels. European Journal of Agronomy, 21, 181–192. https://doi.org/10.1016/j.eja.2003.08.001
  19. Hajghani, M., & Galavand, A. (2015). The response of safflower seed quality characteristics to organic and chemical fertilization. Biological Agriculture & Horticulture,32(2),1-9. https:// 1080/01448765.2015.1094674
  20. Hazeri Niri, H., Tobeh, A., Gholipouri, A., Mostafaei, H., & Jamaati-E-Somarin, S. (2010). The effect of nitrogen and phosphorous rates on fertilizer use efficiency in lentil. World Applied Sciences Journal, 9(9), 1043-1046. http://www.idosi.org/.../13.pdf
  21. Hirel, B., Martin, A., Terce-Laforque, T., Gonzalez-Moro, M.B., & Estavillo, J.M. (2005). Physiology of maize I: A comprehensive and integrated view of nitrogen metabolism in a C4 Physiologia Plantarum, 124, 167–177. https://doi.org/10.1111/j.1399-3054.2005.00510.x
  22. Hussain, M., Cheema, S.A., Abbas, R.Q., Ashraf, M.F., Shahzad, M., Farooq, M., & Jabran, K. (2018). Choice of nitrogen fertilizer affects grain yield and agronomic nitrogen use efficiency of wheat cultivars. Journal of Plant Nutrition, 41, 2330-2343. https://doi.org/10.1080/01904167.2018.1509998
  23. Izan, T., Javanmard, A., Shekari, F., & Abbasi, A. (2020). Evaluation of yield, yield components and some physiological traits of sunflower with integrative application of biological, chemical, and organic fertilizers under different irrigation levels. Journal of Agricultural Science and Sustainable Production, 30(3), 87-111. https://1001.1.24764310.1399.30.3.6.1
  24. Kamayestani, N., Rezvani Moghaddam, P., Jahan, M., & Rejali, F. (2015). Effects of single and combined application of organic and biological fertilizers on quantitative and qualitative yield of anise (Pimpinella anisum). Iranian Journal of Field Crops Research, 13(1), 62-70. (In Persian with English Summary). https://22067/JAG.V10I2.32837
  25. Karami Chame, S., Khalil-Tahmasbi, B., ShahMahmoodi, P., Abdollahi, A., Fathi, A., Seyed Mousavi, S.J., & Bahamin, S. (2016). Effects of salinity stress, salicylic acid and pseudomonas on the physiological characteristics and yield of seed beans (Phaseolus vulgaris). Scientia Iranica, 14(2), 234-238. https://15192/PSCP.SA.2016.14.2.234238
  26. Koocheki, A.,  Nasiri Mahallati, M.,  Moradi, R., &  Alizadeh, Y. (2015). Evaluation of yield and nitrogen use efficiency of maize and cotton intercropping under different nitrogen levels. Field Crops Research, 13(34), 1-13. (In Persian with English Summary). https:// 22067/GSC.V13I1.48310
  27. Limon-Ortega, A., Govaerts, B., & Sayre, K.D. (2008). Straw management, crop rotation, and nitrogen source effect on wheat grain yield and nitrogen use efficiency. European Journal of Agronomy, 29, 21–28. https://doi.org/10.1016/j.eja.2008.01.008
  28. MAJ (Ministry of Agriculture Jihad). (2017). Agriculture Statistical Year Book, First volume, crops. 2015-2016. http://www.maj.ir.
  29. Malakooti, M.J., & Baba Akbari, M. (2005). The need to increase the efficiency of nitrogen fertilizers in the Iran country. Part I. Practical definitions and examples. Technical Journal 425, pp 34. Sena Publications, Tehran, Iran. (In Persian). https://press-swri.areeo.ac.ir/book_2226.html
  30. Mirzakhani, M., Ghanbari Kashan, M., & Hashemi, S.A.F. (2016). Response of nitrogen physiological efficiency of safflower to animal and chemical fertilizers in Kashan region. Journal of Plant Environmental Physiology, 11(41), 53-64. (In Persian with English Summary) https://1001.1.76712423.1395.11.41.5.8
  31. Moghimi, F., & Yousefi Rad, M. (2013). The effects of nitroxin (Azotobacter) usage on yield and yield components of safflower (cv.Goldasht) in the presence of EDTA. Journal of Plant Ecophysiology, 5(13), 39-47. (In Persian). https://sid.ir/paper/188406/fa
  32. Mohammadian, M., Astaraie, A., Lakzian, A., Emami, H., & Kavousi, M. (2018). Effect of nitrogen supplying sources on nitrogen use efficiency in rice (Shiroudi cultivar). Iranian Journal of Crop Sciences, 21(1), 82-95. (In Persian with English Summary) https://sid.ir/paper/385333/fa
  33. Motesharezadeh, B., Valizadeh-Rad, K., Dadrasnia, A., & Amir-Mokri, H. (2017). Trend of fertilizer application during the last three decades (Case study: America, Australia, Iran and Malaysia). Journal of Plant Nutrition, 40(4), 532-542. https://doi.org/10.1080/01904167.2016.1250909
  34. Nasirzade, S., Fallah, S., Kiani, S.H., & Mohammadkhani, A., (2015). Effect of differnt levels of cow manure and urea on quantitative and qulitative characteristics of isabgol (Plantago Ovata Forssk.). Iranian Journal of Medicinal and Aromatic Plants, 31(1), 41-51. (In Persian with English Summary) https://doi.org/10.22092/ijmapr.2015.12609
  35. Pathak, R.R., Ahmad, A., Lochab, S., & Raghuram, N. (2008). Molecular physiology of plant Nitrogen use efficiency and biotechnological option for its enhancement. Journal of Current Science, 94: 1394-1403. http://www.jstor.org/stable/24100496
  36. Ozturk, A., Caglar, O., & Sahin, F., (2003). Yield response of wheat and barley to inoculation of plant growth promoting rhizobacteria at various levels of nitrogen fertilization. Journal of Plant Nutrition and Soil Science, 166(2), 262-266. https://doi.org/10.1002/jpln.200390038
  37. Parsa, S., Kafi, M., & Nassiri, M. (2009). Effects of salinity and nitrogen levels on nitrogen content of wheat cultivars (Triticum aestivum). Scientific information Database, 7(2), 347-355. (In Persian with English Summary) https://sid.ir/paper/119162/fa.
  38. Pirasteh Anosheh, H., Emam, Y., & Jamali Ramin, F. (2010). Comparative effect of biofertilizers with chemical fertilizers on sunflower (Helianthus annuus) growth, yield and oil percentage in different drought stress levels. Journal of Agroecology, 2(3), 492-501. (In Persian with English Summary) https://sid.ir/paper/474441/fa
  39. Qureshi, A., D.K. Singh & Dwivedi, S. (2018). Nano-fertilizers: A Novel Way for Enhancing Nutrient Use Efficiency and Crop Productivity. International Journal of Current Microbiology and Applied Sciences, 7(2), 3325- 3335. https://doi.org/10.20546/ijcmas.2018.702.398
  40. Rabiei, M., & Tousi Kahel, P. (2011). Effects of nitrogen and potassium fertilizer levels on nitrogen use efficiency and yield of rapeseed (Brassica napus ) as a second crop after rice in Guilan region. Iranian Journal of Field Crop Science, 42(3), 605-615(In Persian with English Summary) 20.1001.1.20084811.1390.42.3.18.8
  41. Rezvani Moghaddam, P.,  Seyedi, S.M., &  Azad, M. (2014). Effects of organic, chemical and biological sources of nitrogen on nitrogen use efficiency in black seed (Nigella sativa). Medicinal and Aromatic Plants, 30(2), 260-274. (In Persian with English Summary) https://doi.org/10.22092/ijmapr.2014.6196
  42. Salvagiotti, F., Castellarin J.M., Miralles, D.J., & Pedrol, H.M. (2009). Sulfur fertilization
    improves nitrogen use efficiency in wheat by increasing nitrogen uptake. Field Crops
    Research
    . 113: 170–177. https://doi.org/10.1016/j.fcr.2009.05.003
  43. Scott, N., Chen, H., & Cui, H. (2018). Nanotechnology Applications and Implications of Agrochemicals toward Sustainable Agriculture and Food Systems. Journal of Agricultural and Food Chemistry, 66(26), 6451-6456. https:// 1021/acs.jafc.8b00964
  44. Sedri, M.H., Golchin, A., Mirkhani, R., Fieziasl, V., & Sioseh-mardeh, A. (2017). Effect of nitrogen application management on nitrogen use efficiency in rainfed wheat using 15N Isotope. Iranian Journal of Soil Research, 31(1), 1-17. (In Persian with English Summary) https://22092/IJSR.2017.109931
  45. Seyyedi, S.M., Khajeh-Hosseini, M., Rezvani Moghaddam, P., & Shahandeh, H. (2015). Relation between the increasing soluble phosphorus and nitrogen uptake and its effects on phosphorus harvest index of black seed. Iranian Journal of Field Crop Science, 46, 25–36. (In Persian with English Summary). https:// 22059/IJFCS.2015.54043
  46. Sureshbabu, K., Amaresan, N., & Kumar, K. (2016). Amazing Multiple Function Properties of Plant Growth Promoting Rhizobacteria in the Rhizosphere Soil. International Journal of Current Microbiology and Applied Sciences, 5(2), 661-683. https:// 20546/ijcmas.2016.502.074.
  47. Sumbul, A.,  Ansari, R.A., Rizvi, R., & Mahmood, I. (2020). Azotobacter: A potential bio fertilizer for soil and plant health management. Saudi Journal of Biological Science, 27(12), 3634–3640. https://doi.org/10.1016/j.sjbs.2020.08.004
  48. Tahmasbpour, B., Younessi-Hamzekhanlu, M., Mahdavisafa, D., & Sabzi Nojadeh, M. (2017). Grain yield performance of Carthamus tinctorius cultivars under water deficient condition. Journal of Biological and Environmental Sciences, 235-243. https://innspub.net/grain-yield-performance-of-carthamus-tinctorius-l-cultivars-under-water-deficient-condition/
  49. Tavakoli Kakhki, H., Nassiri Mahallati, M., Koocheki, A., Jahan, M., & Beheshti, S.A.R. (2020). Evaluation of Nitrogen Balance in Wheat (Triticum aestivum) Cropping Systems of Iran. Journal of Agroecology, 12(3), 523-540. https:// 20.1001.1.20087713.1399.12.3.10.6
  50. VtJnvnl,qrssMr, K., Snrcnr, N. K., PrlroN, W. L., & No ANornsoN, C. H. (1975). Effects of plant population and row spacing on sunflower agronomy. Canadian Journal of Plant Science, 55, 491-499. https://doi.org/10.4141/cjps75-075
  51. Warman, P.R., & Termeer, W.C. (2005). Evaluation of sewage sludge, septic waste and sludge compost applications to corn and forage: Ca, Mg, S, Fe, Mn, Cu, Zn and B content of crops and soils. Bioresource Technology, 96(9), 1029-1038. https://doi.org/10.1016/j.biortech.2004.09.014
  52. Weih, M., Asplund, L., & Bergkvist, G. (2011). Assessment of nutrient use in annual and perennial crops: A functional concept for analyzing nitrogen use efficiency. Plant and Soil, 339(1-2), 513-520.
    https://doi.org/10.1007/s11104-010-0599-4
  53. Weiss, E.A. (2000). Oilseed Crops. Blackwell Science Ltd, Second Edition, 364 pp. https://doi.org/10.1017/S0014479701331058
  54. Wu, L., Yuan, S., Huang, L., Sun, F., Zhu, G., Li, G., Fahad, S., Peng, S., & Wang, F. (2016). Physiological mechanisms underlying the high-grain yield and high-nitrogen use efficiency of elite rice varieties under a low rate of nitrogen application in China. Frontiers in Plant Science, 7, 1-12. https://3389/fpls.2016.01024
  55. Yang, Y.C., Zhang, M., Zheng, L., Cheng, D.D., Liu, M., & Geng, Y.Q. (2011). Controlled
    release urea improved nitrogen use efficiency, yield, and quality of wheat. Agronomy
    Journal
    , 103(2): 479-485.  https://doi.org/10.2134/agronj2010.0343

 

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