اثر تنظیم‌کننده‌های زیستی بر صفات مورفولوژیک، فیزیولوژیک و ترکیبات اسانس Ammi visnaga (L.)

نوع مقاله : علمی - پژوهشی

نویسندگان

1 گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه زنجان، ایران

2 گروه زراعت، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران

3 گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران

چکیده

به­منظور بررسی تأثیر تنظیم­کننده­های زیستی بر صفات مورفولوژیک، فیزیولوژیک و ترکیبات اسانس گیاه داروییAmmi visnaga (L.)  آزمایشی در قالب طرح بلوک­های کامل تصادفی در دانشگاه زابل در سال زراعی 94-1393 انجام گرفت. تیمارهای استفاده شده در این پژوهش شامل اسید سالیسیلیک، اسید سینامیک و اسید بنزوئیک با سه سطح 5، 10 و 20 میلی­گرم بر لیتر و اسید آمینه­های فنیل آلانین و تیروزین با سه سطح 50، 100 و 200 میلی­گرم بر لیتر بودند. نتایج نشان داد که تنظیم­کننده­های زیستی باعث افزایش میزان صفات مورد بررسی شدند. همچنین تیمار اسید بنزوئیک 20 میلی­گرم بر لیتر بیشترین تأثیر را به نسبت سایر تیمارها داشت؛ به طوری­که باعث افزایش معنی­داری در رشد رویشی (16/46 درصد)، محتوای آب نسبی (17/60 درصد)، رنگیزه­های فتوسنتزی (کلروفیل a، کلروفیل b، کارتنوئید، کلروفیل  a+ کلروفیل b و (کلروفیل  a+ کلروفیل b)/ کارتنوئید به­ترتیب 63/77، 6/60، 67/66، 32/73 و 12/19 درصد)، هیدرات­های کربن (43/53 درصد)، عملکرد میوه (1/60 درصد) و درصد و عملکرد اسانس (78/72 و 28/32 درصد) به نسبت شاهد شد. نتــایج حاصل از ایــن تحقیــق بیان داشت کــه کاربرد تنظیم­کننده­های زیستی بـا هـدف کـاهش مصرف کودهای شـیمیایی، در افزایش عملکرد کمـی و کیفـی گیـاه دارویـی Ammi visnaga و همچنـین پایداری تولیــد و حفــظ محـیط­زیـست تــأثیر مثبتــی داشته است.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of Bio-Regulators on Morphological and Physiological Traits and Essential Oil of Ammi visnaga (L.) Lam

نویسندگان [English]

  • Diako Rasouli 1
  • Roghayeh Mohammadpour Vashvaei 2
  • Barat Ali Fakheri 3
1 Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, Iran
2 Department of Agronomy, Faculty of Agriculture, University of Zabol, Zabol, Iran
3 Department of Plant Breeding and Biotechnology , Faculty of Agriculture, Zabol University, Zabol, Iran
چکیده [English]

Introduction[1]
Ammi visnaga (L.) Lam is a herbaceous medicinal plant and belongs to Umbelliferae family. It is native to the Mediterranean region. A. visnagais well known as a source of essential oil and is especially cultivated for it therapeutic properties (diaphoretic, carminative, antispasmodic, antiseptic, tonic,) being used in traditional medicine systems in many countries. Essential oil of A. visnaga is known for its proprieties against coronary diseases and bronchial asthma. Phenolic compounds considered as a kind of bio-regulators which are synthesized in the environmental conditions in plant cells. These compounds are involved in various processes of plant growth and reproduction as well as a defense mechanism against biotic and abiotic stresses. Amino acids as bio-regulators have been identified as an alternative to chemical fertilizer to increase soil fertility and crop production in sustainable farming. Therefore, the main objective of the present field experiment was to investigate the effects of bio-regulators on morphological and physiological traits and essential oil of A. visnaga.
 
Materials and Methods
A factorial experiment was performed based on a randomized complete block design (RCBD) at Agricultural Research Institute of Zabol University in 2014-15. The experiment was conducted in pots with a height of 20 cm and a diameter of 33 cm. The treatments used in this study consisted phenolic compounds (salicylic acid, trans-cinnamic acid and benzoic acid with three levels of 5, 10 and 20 mg l-1) and amino acids (phenylalanine and tyrosine with three levels of 50, 100 and 200 mg l-1). Distilled water was used as control. All treatments were applied by foliar application and spraying was done 30 days after planting. Measurement was performed at maturity stage (210 days after planting) and fruiting (180 days after planting). The measured traits include vegetative growth characteristics (plant height, branch number, umbel number, fresh weight of herb and dry weight of herb), relative water content (RWC), photosynthetic pigments (Chl a, Chl b, carotinoids, Chl a+Chl b and (Chl a+Chl b)/carotinoids), carbohydrate (total carbohydrates, soluble carbohydrate, insoluble carbohydrate), Fruit yield, essential oil content and yield. For identifying the essential oil components, essential oil fraction was collected and subjected to GC/MS (Gas Chromatography-Mass spectrometry) analysis. Analysis of variance by using SAS software and mean comparisons by Duncan’s multiple range test (at the 5% probability level) was done.
 
Results and Discussion
The results indicated that bio-regulators significantly affected on all of the traits.  In addition, benzoic acid 20 mg l-1 had the greatest impact compared to other treatments so that, vegetative growth characteristics 46.2 percent, RWC 60.2 percent, photosynthetic pigments include Chl a 77.6, Chl b 60.6, carotinoids 66. 7, Chla+Chl b 73.3 and Chl a+Chl b/carotinoids 19.1percent were increased compared to control treatment. After extraction type and amount of volatile compounds were determined in the aerial part of A. visnaga with GC-MS. Dominant compounds of essential oil in this plant were included 2, 2-dimethylbutanoic acid, isobutyl isobutyrate, thymol and croweacin. In this study, all treatments on the green tissues of A. visnaga were increased the essential oil content. The results of this study demonstrated that, the use of bio-regulators, with aimed at reducing the use of chemical fertilizers, had a positive effect to increase the quality and quantity of A. visnaga and also, sustainable production and environmental protection.
 
Conclusion
The results of this study demonstrated that, the use of bio-regulators, with aimed at reducing the use of chemical fertilizers, had a positive effect to increase the quality and quantity of A. visnaga and also, sustainable production and environmental protection. Considering the importance of the production of medicinal plants in farming systems, bio-regulators such as phenolic compounds seem to be a viable alternative to chemical fertilizers in the production of these plants.
 

کلیدواژه‌ها [English]

  • Amino acids
  • Bio-regulators
  • Essential oil
  • Phenolic compounds
Adams, R.P. 2001. Identification of Essential Oil Components by Gas Chromatography/ uadrupole Mass Spectroscopy. Allured: Carol Stream. USA.
Adisakwattana, S., Sompong, W., Meeprom, A., Ngamukote, S., and Yibchok-Anun, S. 2012. Cinnamic acid and its derivatives inhibit fructose-mediated protein glycation. International Journal of Molecular Sciences 13: 1778-1789.
Astarai, A., and Koocheki, A. 1997. The Use of Biological Fertilizers in Sustainable Agriculture. Mashhad ID Press, Mashhad, Iran. (In Persian)
Belde, M., Matteis, A., Sprengle, B., Albrecht, B., and Hurle, H. 2000. Long- term development of yield affecting weeds after the change from conventional to integrated and organic farming. In: proceeding 20 German Conference on weed Biology and Weed Control 17: 291-301.
Bown, D. 1995. Encyclopaedia of Herbs and their Uses. Dorling Kindersley, London, ISBN 0-7513-020-31.
Charles, D.J., Joly, R.J., and Simon, J.E. 1990. Effect of osmotic stress on the essential oil content and composition of peppermint. Photochemistry 29(9): 2837–2840.
Ghasemzadeh, A., and Jaafar, H. 2012. Effect of salicylic acid application on biochemical changes in ginger (Zingiber officinale Roscoe). Journal of Medicinal Plants Research 6: 790–795.
Chevalier, A. 1996. The Encycclopedia of Medicinal Plants. Dorling Kindersley, London. ISBN 9-780751-303148.
Cohen, S., and Kennedy, J. 2010. Plant metabolism and the environment: Implications for managing phenolics. Food Science and Nutrition 50: 620–643.
Erisman, J.W. 2004. The Nanjing declaration on management of reactive nitrogen. Bioscience 54: 4286-4287.
Garde-Cerdan, T., Lopez, R., Portu, J., Gonzalez-Arenzana, L., Lopez-Alfaro, I., and Santamaria, P. 2014. Study of the effects of proline, phenylalanine, and urea foliar application to Tempranillo vineyards on grape amino acid content. Comparison with commercial nitrogen fertilizers. Food Chemistry 163: 136-141.
Hamidi, A., Ghalavand, A., Dehghan, M., Malakuti, M.J., Asgharzade, A., and Chokan, R. 2005. The effect of application of plant growth promoting rhizobacteria on the yield of fodder maize (Zea mays L.). Pajouhesh and Sazandegi 70: 16-22. (In Persian with English Summary)
Ibrahim, M., and Jaafar, H. 2011. Involvement of carbohydrate, protein and phenylanine ammonialyase in up-regulation of secondary metabolites in Labisia pumila under various CO2 and N2 levels. Molecules 16: 4172–4190.
Jamshidi, M., Ahmadi, H.R., Rezazadeh, S.h., Fathi, F., and Mazanderani, M. 2010. Study on phenolicd and anioxidant activity of some selected plant of Mazandaran province. Journal of Medical Planats 9(34): 177-183. (In Persian with English Summary)
Jiang, Y., and Huang, N. 2001. Drought and heat stress injury to two cool-season turfgrasses in relation to antioxidant metabolism and lipid peroxidation. Crop Science 41: 436-442.
Kang, C., and Wang, C.H. 2003. Salicylic acid changes activities of H2O2 metabolizing enzymes and increases the chilling tolerance of banana seedlings. Environment and Experimental Botany 9-15.
Khadhri, A., El Mokni, R., Mguis, K., Ouerfelli, I., and Eduarda, M.M.A. 2011. Variability of two essential oils of Ammi visnaga (L) Lam. a traditional Tunisian medicinal plant. Journal of Medicinal Plants Research 5(20): 5079–5082.
Lichtenthaler, H.K. 1987. Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. Methods in Enzymology 148: 350-382.
Lrigoyen, J.J., and Emerich, D.W. 1992. Water stress induced changes in concentration of praline and total soluble sugars in modulates alfalfa (Medicago satire) plants. Physiologic Planetarium 84: 55-60.
Myung-Min, H., Trick, H.N., and Rajasheka, E.B. 2009. Secondary metabolism and antioxidant are involved in environmental adaptation and stress tolerance in lettuce. Journal of Plant Physiology 166: 180-191.
Nagarja, G., Gowda J., and Farooqi, A. 1999. Effect of growth regulators on growth and flowering of Tuberose cv. Single. Karantaka Journal of Agriculture Science 12: 234-238.
Nardi, S., Pizzeghello, D., Muscolo, A., and Vianello, A. 2002. Physiological effects of humic substances on higher plants. Soil Biology and Biochemistry 34: 1527-1536.
Nosengo, N. 2003. Fertilized to death. Nature 425: 894.895.
Ozturk, A., Unlukara, A., Ipek, A., and Gurbuz, B. 2004. Effects of Salt Stress and Water Deficit on Plant Growth and Essential oil Content of Lemon Balm (Melissa officialis L.). Pakistan Journal of Botany 36(4): 787-792.
Perez, M.G.F., Rocha-Guzman, N.E., Mercado-Silva, E., Loarca-Piña, G., and Reynoso-Camacho, R. 2014. Effect of chemical elicitors on peppermint (Mentha piperita) plants and their impact on the metabolite profile and antioxidant capacity of resulting infusions. Food Chemistry 156: 273-278.
Pessarakli, M. 1999. Handbook of Plant and Crop Stress. Marcel Dekker Inc.
Preeti, H., and Gogoi, S. 1997. Effects of preplant chemical treatment of bulbs on growth and flowering of Polianthes tuberosa cv. Single. Annuals Biology 13: 145-149.
Rezvani Moghaddam, P., Raoofi, M.R., Rashed Mohassel, M.H., and Moradi, R. 2009. Evaluation of sowing patterns and weed control on mung bean (Vigna radiate L. Wilczek)- black cumin (Nigella sativa L.) intercropping system. Journal of Agroecology 1(1): 65-79. (In Persian with English Summary)
Rice-Evans, C.A., Miller, N.J., and Paganga, G. 1997. Antioxidant properties of phenolic compounds. Trends Plant Science 2: 152-159.
Rose, J., and Hulburd, J. 1992. The Aromatherapy Book Applications and Inhalations. North Atlantic Books, Health & Fitness 375 pp.
Sajjadi Niaki, H., Darzi, M.T., and Haj Seyed Hadi, M.R. 2016. Effects of vermicompst and nitroxin biofertilizer on quantity and quality of essential oil of dragonhead (Dracocephalum moldavica L.). Journal of Agroecology 8(2): 241-250. (In Persian with English Summary)
Satrani, B., Farah, A., Fechtal, M., Talbi, M., and Bouamri, M.L. 2004. Chemical composition and antimicrobial and antifungal activities of the essential oil of Ammi visnaga (L.) Lam from Marocco. Acta Botanica Gallica 151 (1): 65–71.
Taguchi, G., Yazawa, T., Hayashida, N., and Okazaki, M. 2001. Molecular cloning and heterologous expression of novel glucosyltransferases from tobacco cultured cells that have broad substrate specificity and are induced by salicylic acid and auxin. European Journal of Biochemistry 268(14): 4086-4094.
Uphof, J.C.T. 1959. Dictionary of Economic Plants. Science 890 pp.
Wu, S.C., Caob, Z.H., Lib, Z.G., Cheunga, K.C., and Wong, M.H. 2005. Effects of biofertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth: A greenhouse trial. Geoderma 125: 155–166.
Yang, R.X., Gao, Z.G., Liu, X., Yao, Y., Cheng, Y., Huang, J., and McDermott, M.I. 2015. Effects of phenolic compounds of muskmelon root exudates on growth and pathogenic gene expression of Fusarium oxysporum f. sp. melonis. Allelopathy Journal 35(2): 175-186.
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