بررسی ویژگی‌های مورفولوژیکی، عملکردی و فیتوشیمیایی گیاه دارویی پریلا ارغوانی (Perilla frutescens var. crispa) در سه مکان مختلف کشت

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه کشاورزی، پژوهشکده گیاهان و مواد اولیه دارویی دانشگاه شهید بهشتی، تهران، ایران.

2 گروه کشاورزی، دانشگاه لرستان، خرم آباد، ایران و پژوهشگر جهاد دانشگاهی واحد استان لرستان، ایران.

3 پژوهشکده گیاهان و مواد اولیه دارویی دانشگاه شهید بهشتی، تهران، ایران.

4 گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران.

چکیده

به‌منظور ارزیابی سازگاری، رشد و برخی خصوصیات کمّی و کیفی گیاه دارویی پریلا ارغوانی (Perilla frutescens var. crispa) در ایران، این گیاه در سه مکان مختلف شامل ساری، رشت و شمال تهران کشت گردید. کشت گیاه دارویی پریلا ارغوانی در قالب طرح کامل تصادفی با سه تکرار در سه منطقه ساری، رشت و شمال تهران در اردیبهشت 1397 انجام شد. گیاهان در شروع گل‌دهی در مهر ماه برداشت شدند. ویژگی‌های مورفولوژیکی و عملکردی مورد مطالعه شامل ارتفاع بوته، طول گل‌آذین، تعداد ساقه، فاصله میان‌گره، قطر ساقه، عرض برگ، طول برگ، وزن تر و خشک برگ، وزن تر و خشک ساقه، وزن تر و خشک گل بودند. صفات فیتوشیمایی مورد مطالعه شامل محتوای فنل و فلاونوئید کل و میزان رزمارینک اسید بودند. نتایج تجریه واریانس نشان داد که اثر مکان‌های مختلف کشت بر همه صفات مورفولوژیکی (به‌جز وزن تر ساقه، وزن تر و خشک بوته) و صفات فیتوشیمیایی در سطح پنج درصد معنی‌دار بود. عملکرد خشک اندام دارویی (برگ و گل) در مکان­های کشت ساری (63/41و 05/32)، رشت (13/26 و71/12) و شمال تهران (99/25 و 47/ 18) گرم در مترمربع به‌دست آمد. محتوای فنل کل و رزمارینیک اسید گیاهان در رشت (به‌ترتیب مقدار027/ 49 و 29/42 میلی‌گرم بر گرم ماده خشک) بیشتر از سایر مناطق کشت بود، در حالی که فلاونوئید کل در منطقه ساری بیشترین مقدار (8/14 میلی‌گرم بر گرم ماده خشک) بود. با در نظر داشتن صفات تولیدی و فیتوشیمایی، رشت و ساری (شمال کشور) مناطق مناسبی برای کشت و تولید گیاه پریلا ارغوانی می‌باشند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Study of Agro-Morphological and Phytochemical Traits of Purple Perilla (Perilla frutescens var. Crispa) Grown in Three Locations

نویسندگان [English]

  • Masume Eidvazaee 1
  • Abuzar Alizadeh 2
  • Samad Nezhad Ebrahimi 3
  • Amir Sahra roo 4
  • Mohammad Hossein Mir Jalili 3
  • Javad Hadian 3
1 Department of Agriculture, Medicinal Plants and Drug Research Institute, Shahid Beheshti University, Tehran, Iran
2 Department of Agriculture, Lorestan University, Khorramabad, Iran and Academic Jihad Researcher of Lorestan Province Unit.
3 Department of Agriculture, Medicinal Plants and Drug Research Institute, Shahid Beheshti University, Tehran, Iran.
4 Department of Agriculture, Medicinal Plants and Drug Research Institute, Shahid Beheshti University, Tehran, Iran
چکیده [English]

Introduction
Medicinal plants are used in all civilizations and cultures are one of the most essential defensive lines to protect human health and fight human diseases in the world. From the distant past to now, the plants have long been considered as a source of renewable medicines.
Perilla (Perilla frutescens var. crispa)is an annual plant native to East Asian, which used as a medicinal plant due to the presence of active metabolite, especially rosmarinic acid. The medicinal organs used in this plant are its leaves and flowering branches. Growth, drug yield, as well as quantity and quality of secondary metabolites of medicinal plants are affected by ecological factors. The present study was conducted to evaluate the agro-morphological and phytochemical traits of Purple Perilla grown in three different locations of Sari, Rasht, and north of Tehran.
Materials and methods:   
The experiment was conducted as a completely randomized design (CRD) with three replications. Purple Perilla  were planted as seedlings in early spring in the trial field with the density of 25×50 cm (inter-plant space × inter-row space) in three locations including Sari, Rasht, and north of Tehran in 2018. Plants were harvested at the flowering stage in late September. Argo-morphological and phytochemical traits including plant height, inflorescence length, number of branches, stem diameter, internode length, leaf length, leaf width, plant fresh weight, stem fresh weight, leaf fresh weight, flowers fresh weight, plant dry weight, leaf dry weight, flower dry weight, total phenolics and flavonoids as well as rosmarinic acid content were measured. The total phenolic contents of extracts of Perilla plants were determined according to the method of Ciocalteus Reagent (FCR) Folinthe aluminum chloride method was used for the determination of the total flavonoid content of the extracts. The rosmarinic acid content was estimated with method HPLC. Data set were subjected to variance analysis using SPSS statistical software (version 19). Also, Duncan’s multiple range test was used to compare treatment means at P<0.05.
Results and Discussion
 Results of variance analysis showed that almost all morphological traits (except stem fresh weight, plant dry weight, and plant fresh weight) and all phytochemical traits were significantly (P<0.05) influenced by locations. Mean comparisons of the effect of different locations for morphological traits showed that the maximum plant height (137.67 cm), leaf length (15.28 cm), fresh weight of leaf (108.3 g), dry weight of leaf (41.63 g) were observed in Sari, while the minimum plant height (87.34 cm), leaf length (10.8 cm), fresh weight of leaf (69.74 g), dry weight of leaf (26.13 g) were detected in north of Tehran. The Rasht had the highest flower fresh weight, flower dry weight, and number of branches with an average of 108.67 g, 25.99 g, and 33, respectively. The lowest flower fresh weight, flower dry weight, and number of branches were observed in the north of Tehran with an average of 47.07, 12.71, and 22 g, respectively. The results revealed that the highest internode length, inflorescence length, and leaf width belonged to the Sari with an average of 9.12, 13.17, and 98.8 mm, respectively, while the Rasht had the lowest internode length, inflorescence length, and leaf width with an average of 6.28, 9.8, and 6.97 mm, respectively. Also, the maximum and minimum stem diameter were observed in the north of Tehran and Sari (8.95 and 7.65 mm), respectively. Mean comparisons of the effect of different locations for phytochemical traits showed that the maximum total phenolic contents and rosmarinic acid produced in the Rasht with an average of 49.03 and 52.20 (mg/ g dry matter), respectively, while the Sari had the maximum flavonoids content (14.8 mg/ g dry matter).
 Conclusion
       Drug yield (leaf and flower fraction) of the plants grown in Sari, Rasht, and north of Tehran was 41.63, 32.05, 26.13 and 12.71, 25.99, 18.47, respectively The highest total phenolics (49.027 mg/ g dry matter) and rosmarinic acid content (46.99 mg/ g dry matter) were observed in Rasht region, while the highest total flavonoids was observed in Sari (14.8 mg/ g dry matter).  In terms of argo-morphological and phytochemical traits, Sari and Rasht were suitable places for the growth of purple perilla.
 

 
 
 

کلیدواژه‌ها [English]

  • Medicinal plant
  • Rosmarinic acid
  • Total phenolic and flavonoids
  • Essential oil
Akbrian, A., Rahimmalek, M., Sabzalian, M., and Saeidi, G., 2017. Assessment of phytochemical, morphological and antioxidant variation of bilehar (Dorema aucheri) populations cultivated in different environmental conditions. JMP 2(62): 120-135. (In Persian with English Summary)
Ciocarlan, N., Sirbu, T., Stefanache, C., Ghendov, V., Necula, R., and Grigoras, V., 2014. Biological and phytochemical research on Perilla frutisence var. purputascens (HAYATA) H.W.LI in Republic of Moldova. Fiziologia şi Biochimia Plantelo. Nr. 2(323)
Cseke, L.J., Kirakosyan, A., Kaufman, P.B., Warber, S.L., Duke, J.A., and Brielmann, H.L., 2006. Natural Products from Plants 2th Ed. CRC Press, Florida, 569 p.
Echeverrigaray, S., Fracaro, F., Dos-Santos, A.C.A., Paroul, N., Wasum, R., and Serafini, L.A., 2003. Essential oil composition of south Brazilian populations of Cunila galioides and its relation with the geographic distribution. Biochemical Systematics and Ecology 31: 467–475. https://doi.org/10.1016/S0305-1978(02)00175-8.
Esmaeili, H., Hadian, J., Mirjalili, M.H., and Rezadoost, H., 2016. Evaluation of some morphological, phytochemical and ecological characteristics of different populations of Satureja rechingeri Jamzad. Journal of Range and Watershed Management 69(1): 1-12. (In Persian with English Summary). https://doi.org/10.22059/jrwm.2016.61729
Feng, L.J., Yu, C.H., Ying, K.J., Hua, J., and Dai, X.Y., 2011. Hypolipidemic and antioxidant effects of total flavonoids of Perilla Frutescens leaves in hyperlipidemia rats induced by high-fat diet. Food Research International 44(1): 404-409.‏ https://doi.org/10.1016/j.foodres.2010.09.035
Gairola, S., Shariff, N., Bhate, A., and Prakash Kola, C., 2010. Influence of climate change on production of secondary chemicals in high altitude medicinal plants. Journal of Medicinal Plant Research 1825-1829. DOI: 10.5897/JMPR10.354.
Ghane, M.R., and Ghajar Sepanlu, M., 2018. Investigation of morphological, yield and medicinal properties of Perilla (Perilla frutscens) under chemical, biological and organic fertilizers in Mashhad Ardehal and Sensen regions of Kashan, Journal of Plant Production 25(2): 99-117. (In Persian with English Summary). https://dx.doi.org/10.22069/jopp.2018.13168.2189
Ghasemi Pirbalouti, A., Hashemi, M., Ghahfarokhi, F.T., 2013. Essential oil and chemical compositions of wild and cultivated Thymus daenensis Celak and Thymus vulgaris L. Industrial Crops and Products 48: 43–48. https://doi.org/10.1016/j.indcrop.2013.04.004
Ghorbani, R., 2003. Organic agriculture and food production: Ecological, environmental, food safety and nutritional quality issues. Sociology, Organic Farming, Climate Change and Soil Science. Springer, Dordrecht p. 77-107.
Guan, Z., Li, S., Lin, Z., Yang, R., Zhao, Y., Liu, J., Yang, S., and Chen, A., 2014. Identification and quantification of phenolic compounds from the seed and pomace of Perilla frutescens using HPLC/PDA and HPLC-ESI/QTOF/MS/MS. Phytochemical Analysis. 25: 508-513. https://doi.org/10.1002/pca.2521
Ha, T.H., Lee, J.H., Lee, M.H., Lee, B.W., Kwon, H.S., and Park, C.H., 2012. Isolation and identification of phenolic compounds from the seeds of Perilla frutescens (L.) and their inhibitory activities against a-glucosidase and aldose reductase. Food Chemistry 135: 1317–1403. https://doi.org/10.1016/j.foodchem.2012.05.104
Hemati, K.H., Omidbeigi, R., and Bashiri Sadr, Z., 2003. Effect of climate and harvest time on the qualitative and qualitative characteristics of flavonoids of citrus varieties. Ph.D. Dissertation Tarbiat Modares University, Tehran, Iran.
Hodisan, T., Socaciu, C., Ropan, I., and Neamtu, G., 1997. Carotenoid composition of Rosa canina fruits determined by thin layer chromatography and high-performance liquid chromatography. Pharmaceutical and Biomedical Analysis 16(3): 521-528. https://doi.org/10.1016/S0731-7085(97)00099-X
Horwath, A.B., Grayer, R.J., Keith-Lucas, D.M., and Simmonds, M.S., 2008. Chemical characterization of wild populations of thymus from different climatic regions in southeast Spain. Biochemical Systematics and Ecology 36: 117–133. https://doi.org/10.1016/j.bse.2007.08.009
Jakola, L., and Hohtola, A., 2010. Effect of latitude on flavonoid biosynthesis in plants. Plant, Cell and Environmental 33(8): 1239-1247. https://doi.org/10.1111/j.1365-3040.2010.02154.x
Lee, J.H., Park, K.H., Lee, M.H., Kim, H.T., Seo, W.D., Kim, J.Y., and Ha, T.J., 2013. Identification, characterization, and quantification of phenolic compounds in the antioxidant activity-containing fraction from the seeds of Korean perilla (Perilla frutescens) cultivars. Food Chemistry 136: 843–852. https://doi.org/10.1016/j.foodchem.2012.08.057
Mazandarani, M., Makari, S., and Bajian, G.R., 2011. Evaluation of phytochemical and antioxidant activity in different parts of Herscleum gorganicum Rech.F. in Golestan province, North of Iran. Iranian Journal Plant Physiology 2(2): 381-388.  https://doi.org 10.30495/IJPP.2012.540771
Meng, L., Lozano, Y., Bombarda, I., Gaydou, E., Li, B., 2006. Anthocyanin and flavonoid production from Perilla frutescens: pilot plant scale processing including cross-flow microfiltration and reverse osmosis. Journal Agricultural Food Chemistry. 54: 4297–4303. https://doi.org/10.1021/jf0604079
Meng, L., Lozano, Y., Bombarda, I., Gaydou, E. M., and Li, B., 2009. Polyphenol extraction from eight Perilla frutescens cultivars. Comptes Rendus Chimie 12(5): 602–611. https://doi.org/10.1016/j.crci.2008.04.011
Mirazadi, Z., Pilehvar, B., Meshkat Alsadat, M.H., and Karamian, R., 2012. Site quality and Essential oil composition of Myrtus Communis L. (case study: Cham moord site in Lorestan province). Journal of Agricultural Biotechnology 3(2): 71-79. (In Persian with English Summary)
Munoz-Bertomeu, J., Arrillaga, I., and Segura, J., 2007. Essential oil variation within and among natural populations of Lavandula latifolia and its relation to their ecological areas. Biochemical Systematics and Ecology 35: 479–488. https://doi.org/10.1016/j.bse.2007.03.006
Nooshkam, A., Mumivand, H., Hadian, J., Alemardan, A., and Morshedloo, M.R., 2017. Drug yield and essential oil and carvacrol contents of two species of Satureja (S. khuzistanica Jamzad and S. rechingeri Jamzad) cultivated in two different locations. Journal of Applied Research on Medicinal and Aromatic Plants 6, 126-130. (In Persian with English Summary). https://doi.org/10.1016/j.jarmap.2017.04.002
Parsafar, S., 2017. Evaluation of heterogeneity, growth and yield of some cultivars and populations of Solidago virgaurea L. M.Sc. Thesis, Shahid Beheshti University, Iran. (In Persian with English Summary)
Russo, A., Formisano, C., Rigano, D., Senatore, F., Delfine, S., Cardile, V., and Bruno, M., 2013. Chemical composition and anticancer activity of essential oils of Mediterranean sage (Salvia officinalis L.) grown in different environmental conditions. Food and Chemical Toxicology 55: 42–47. https://doi.org/10.1016/j.fct.2012.12.036.
Samidha, K., Vrushali, K., and Vijaya, P., 2014. Estimation of phenolic content, flavonoid content, antioxidant and alpha amylase inhibitory activity of marketed polyherbal formulation. Journal of Applied Pharmaceutical Science 4(09): 061-065. http://dx.doi.org/10.7324/JAPS.2014.40911.
Sharma, R., Samant, S., Sharma, P., and Devi, S., 2012. Evaluation of antioxidant activity of Withania somnifera leaves growing in natural habitats of North-West Himalaya, India. Journal of Medicinal plants Research 6: 657-661. https://doi.org/10.5897/JMPR11.257.
Shi Jun, M., and Ju Kyong, L., 2017. Morphological variation of two cultivated types of perilla crop from different areas of China. Horticultural Science and Technology 35(4): 510-522.
Srivastava, A.R., Banerjee, A., Jacob, S., and Dunning, J., 2007. Should patients undergoing coronary artery bypass grafting with mild to moderate ischaemic mitral regurgitation also undergo mitral valve repair or replacement?. Interactive Cardiovascular and Thoracic Surgery 6(4): 538-546. https://doi.org/10.1510/icvts.2007.157891.
Telci, I., Kacar, O., Bayram, E., Arabac, O., Demirtas, I., Yilmaz, G., and Göksu, E., 2011. The effect of ecological conditions on yield and quality traits of selected peppermint (Mentha piperita L.) clones. Industrial Crops and Products 34: 1193–1197. https://doi.org/10.1016/j.indcrop.2011.04.010.
Valizadeh, J., Bagheri, A., and Mirjalili, M.H., 2015. Phytochemical investigation of Withania coagulans (Stocks) dunal in natural habits of Iran. Iranian Journal of Medicinal and Aromatics Plants 31: 406-417.
Wojdylo, A., Oszmianski, J., and Czemerys, R., 2007. Antioxidant activity and phenolic compound in 32 selected herbs. Food Chemistry 105(3): 940-949. https://doi.org/10.1016/j.foodchem.2007.04.038.
Yavari, A.R., Nazeri, V., Sefidkon, F., and Hassani, M.E., 2010. Evaluation of some ecological factors, morphological traits and essential oil productivity of Thymus migricus Klokov and Desj.-Shost. Iranian Journal of Medicinal and Aromatic Plants 26(2): 227-238. (In Persian with English Summary). http://dx.doi.org/10.22092/ijmapr.2015.11933
Yazdani, D., Rezazadeh, S., and Shahnazi, C., 2002. A review of the Papaver somniferum plant.  Journal of Medicinal Plants 5: 1-12. (In Persian with English Summary)
Zargari, A., 1992. Medicinal Plants, volume 1, Tehran University Press, Iran. 486. (In Persian)
Zhang, Z.H., VanCoillie, F.M., De Clercq, E., Ou, X., and De Wulf, R., 2013. Mountain vegetation change quantification using surface landscape metrics in Lancang watershed, China. Ecological Indicators 31: 49-58. https://doi.org/10.1016/j.ecolind.2012.11.013
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